Multiphoton lithography, processing and fabrication of photonic structures

Fourkas, John T.

doi:10.1533/9780857096227.2.137

Cited by 3 publications

(1 citation statement)

References 81 publications

(100 reference statements)

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“…Much of the previous and current activity employing MPE fabrication has been in nonbiological applications in polymeric systems [23,24]. The research has been directed at making devices for several applications including 3D memory, photonic crystals, and miniature electronics [25][26][27][28][29]. By comparison, biological applications have been less explored (especially in terms of de novo fabrication with proteins), although they are on the increase.…”

Section: Introductionmentioning

confidence: 99%

Image-inspired 3D multiphoton excited fabrication of extracellular matrix structures by modulated raster scanning

Ajeti¹,

Lien²,

Chen³

et al. 2013

Opt. Express

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Multiphoton excited photochemistry is a powerful 3D fabrication tool that produces sub-micron feature sizes. Here we exploit the freeform nature of the process to create models of the extracellular matrix (ECM) of several tissues, where the design blueprint is derived directly from high resolution optical microscopy images (e.g. fluorescence and Second Harmonic Generation). To achieve this goal, we implemented a new form of instrument control, termed modulated raster scanning, where rapid laser shuttering (10 MHz) is used to directly map the greyscale image data to the resulting protein concentration in the fabricated scaffold. Fidelity in terms of area coverage and relative concentration relative to the image data is ~95%. We compare the results to an STL approach, and find the new scheme provides significantly improved performance. We suggest the method will enable a variety of cell-matrix studies in cancer biology and also provide insight into generating scaffolds for tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Image-inspired 3D multiphoton excited fabrication of extracellular matrix structures by modulated raster scanning

Ajeti¹,

Lien²,

Chen³

et al. 2013

Opt. Express

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Comprehensive Review on Design and Manufacturing of Bio-scaffolds for Bone Reconstruction

Ravoor

Thangavel

Elsen

2021

ACS Appl. Bio Mater.

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Bio-scaffolds are synthetic entities widely employed in bone and soft-tissue regeneration applications. These bio-scaffolds are applied to the defect site to provide support and favor cell attachment and growth, thereby enhancing the regeneration of the defective site. The progressive research in bio-scaffold fabrication has led to identification of biocompatible and mechanically stable materials. The difficulties in obtaining grafts and expenditure incurred in the transplantation procedures have also been overcome by the implantation of bio-scaffolds. Drugs, cells, growth factors, and biomolecules can be embedded with bio-scaffolds to provide localized treatments. The right choice of materials and fabrication approaches can help in developing bio-scaffolds with required properties. This review mostly focuses on the available materials and bio-scaffold techniques for bone and soft-tissue regeneration application. The first part of this review gives insight into the various classes of biomaterials involved in bio-scaffold fabrication followed by design and simulation techniques. The latter discusses the various additive, subtractive, hybrid, and other improved techniques involved in the development of bio-scaffolds for bone regeneration applications. Techniques involving multimaterial printing and multidimensional printing have also been briefly discussed.

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