Multiphoton imaging reveals that nanosecond pulsed electric fields collapse tumor and normal vascular perfusion in human glioblastoma xenografts

Abstract

Despite the biomedical advances of the last century, many cancers including glioblastoma are still resistant to existing therapies leaving patients with poor prognoses. Nanosecond pulsed electric fields (nsPEF) are a promising technology for the treatment of cancer that have thus far been evaluated in vitro and in superficial malignancies. In this paper, we develop a tumor organoid model of glioblastoma and apply intravital multiphoton microscopy to assess their response to nsPEFs. We demonstrate for the first time that a single 10 ns, high voltage electric pulse (35–45 kV/cm), collapses the perfusion of neovasculature, and also alters the diameter of capillaries and larger vessels in normal tissue. These results contribute to the fundamental understanding of nsPEF effects in complex tissue environments, and confirm the potential of nsPEFs to disrupt the microenvironment of solid tumors such as glioblastoma.

Activation of Anti-tumor Immune Response by Ablation of HCC with Nanosecond Pulsed Electric Field

“…Beside HCC, nsPEF has been applied to multiple solid tumors. For example, nsPEF has been shown to enhance the anti-tumor effects of the mTOR inhibitor everolimus against melanoma, 22 to collapse vascular perfusion in glioblastoma, 23 to inhibit proliferation in osteosarcoma, 24 and to serve as breast cancer therapy 25 and against salivary adenoid cystic carcinoma. 26 …”

Chorioallantoic Membrane Models of Various Avian Species: Differences and Applications

“…Bardet et al tested on glioblastoma U87-MG tumour xenografts grown on quail CAM the method for cancer treatment using nanosecond pulsed electric fields [121].…”

Multiphoton microscopy for pre-clinical evaluation of flow-diverter stents for treating aneurysms

“…The nuclei of the samples (rabbit and pig) were stained with DAPI (Sigma-Aldrich) in Live Cell Imaging Solution (Molecular Probes®) for 5 min and then washed twice. A customised multiphoton microscope (BX61WI/ FV1200MPE, Olympus) was coupled with a linear polarisation femtosecond Ti:sapphire pulsed laser (Chameleon Ultra II, Coherent) (7). 3D multichannel stacks (500 × 500 μm) were collected at 2-μm intervals with 512 × 512 pixels and a 12.5-μs/pixel dwell time at 810 nm using FluoView FV1200 software (Olympus).…”

“…Over the last decade, several research groups have provided technical guides for in vivo imaging of vascular compartments using multiphoton microscopy of several organs [5][6][7]. Moreover, recent advances in photonic tools have provided novel results relative to classical histological approaches for assessing tissue architecture due to the ability to image deeper areas and render three-dimensional (3D) views of tissue.…”