Abstract:Multiphoton microscopy allows measurement of network activity as well as the manipulation of cell type specific or functionally identified neuronal subpopulations with optogenetic holographic stimulation. When neurons co-express an activity reporter (e.g. calcium or voltage-sensitive indicators) and an (excitatory or inhibitory) opsin, such all optical interrogation approaches in vivo allows to draw causal links between function of cell-type specific microcircuits and behaviour. However, the net effects of nea… Show more
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