Multiphoton fabrication of chemically responsive protein hydrogels for microactuation

Kaehr, Bryan; Shear, Jason B.

doi:10.1073/pnas.0709571105

Cited by 184 publications

(186 citation statements)

References 44 publications

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“…As another alternative, a spatial light modulator (SLM) can be employed to control the light amplitude and phase to spatially shape the laser beam for multi-focal patterning or single-shot pattern projection 34,39,47,57,58,60,64,65 . Light modulation is achieved either by a digital mirror device (DMD) composed of an array of individually rotatable micro-mirrors, or a liquid crystal display (LCD) composed of an array of individual liquid crystal pixels electrically modulating the light.…”

Section: Fabrication Conditions Lasers and Direct Writing Systemsmentioning

confidence: 99%

“…These structures can be nested on the substrate surfaces 33,34 or embedded into transparent devices such as microfluidic channels [35][36][37] . In addition to 3D fabrication and superior resolution, another advantage of the multi-photon cross-linking of proteins as 3D printing materials is the potential to retain the protein functions after fabrication 35,38,39 . These functions include not only the diverse intrinsic properties of native proteins such as charge transfer, molecule modification and cell-cell communication, but also engineered functions of synthetic proteins.…”

Section: Introductionmentioning

confidence: 99%

“…These functions include not only the diverse intrinsic properties of native proteins such as charge transfer, molecule modification and cell-cell communication, but also engineered functions of synthetic proteins. Consequently, the resulting protein structures have been applied to fabricate functional microdevices, including pH-sensitive actuators 39,40 and optical microcomponents 41,42 . The integration of proteinaceous microstructures into commercial polymers 43 and protein-polymer hybrids 44 has also been found to increase mechanical strength.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Serien¹,

Sugioka²

2018

Opto-Electronic Advances

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Proteins are a class of biomaterials having a vast array of functions, including the catalysis of metabolic reactions, DNA replication, stimuli response and transportation of molecules. Recent progress in laser-based fabrication technologies has enabled the formation of three-dimensional (3D) proteinaceous micro-and nano-structures by femtosecond laser cross-linking, which has expanded the possible applications of proteins. This article reviews the current knowledge and recent advancements in the femtosecond laser cross-linking of proteins. An overview of previous studies related to fabrication using a variety of proteins and detailed discussions of the associated mechanisms are provided. In addition, advances and applications utilizing specific protein functions are introduced. This review thus provides a valuable summary of the 3D micro-and nano-fabrication of proteins for biological and medical applications.

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Section: Fabrication Conditions Lasers and Direct Writing Systemsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Serien¹,

Sugioka²

2018

Opto-Electronic Advances

View full text Add to dashboard Cite

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“…With current technology and materials, creating nanostructured materials in complex shapes requires either multiple steps (e.g. alignment followed by assembly), or dual development of materials and manufacturing (for example, alignment of fibre constructs during additive manufacture) [138,140,143].…”

Section: Resultsmentioning

confidence: 99%

“…These gels turn quasiliquid when exposed to selected pH levels which remove one network, but can recover their pre-programmed shapes using the secondary duplex DNA network [138,139]. Microscale DNA-responsive hydrogel devices have been developed that are capable of bending in response to their chemical environment, and even selectively detecting, trapping and releasing bacterial cells [140].…”

Section: Chemicalmentioning

confidence: 99%

Morphing in nature and beyond: a review of natural and synthetic shape-changing materials and mechanisms

2016

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Shape-changing materials open an entirely new solution space for a wide range of disciplines: from architecture that responds to the environment and medical devices that unpack inside the body, to passive sensors and novel robotic actuators. While synthetic shape-changing materials are still in their infancy, studies of biological morphing materials have revealed key paradigms and features which underlie efficient natural shape-change. Here, we review some of these insights and how they have been, or may be, translated to artificial solutions. We focus on soft matter due to its prevalence in nature, compatibility with users and potential for novel design. Initially, we review examples of natural shape-changing materials-skeletal muscle, tendons and plant tissues-and compare with synthetic examples with similar methods of operation. Stimuli to motion are outlined in general principle, with examples of their use and potential in manufactured systems. Anisotropy is identified as a crucial element in directing shape-change to fulfil designed tasks, and some manufacturing routes to its achievement are highlighted. We conclude with potential directions for future work, including the simultaneous development of materials and manufacturing techniques and the hierarchical combination of effects at multiple length scales.

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Preparation of Hydrogel Substrates with Tunable Mechanical Properties

2010

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The modulus of elasticity of the extracellular matrix (ECM), often referred to in a biological context as “stiffness,” naturally varies within the body, e.g., hard bones and soft tissue. Moreover, it has been found to have a profound effect on the behavior of anchorage‐dependent cells. The fabrication of matrix substrates with a defined modulus of elasticity can be a useful technique to study the interactions of cells with their biophysical microenvironment. Matrix substrates composed of polyacrylamide hydrogels have an easily quantifiable elasticity that can be changed by adjusting the relative concentrations of its monomer, acrylamide, and cross‐linker, bis‐acrylamide. In this unit, we detail a protocol for the fabrication of statically compliant and radial‐gradient polyacrylamide hydrogels, as well as the functionalization of these hydrogels with ECM proteins for cell culture. Included as well are suggestions to optimize this protocol to the choice of cell type or stiffness with a table of relative bis‐acrylamide and acrylamide concentrations and expected elasticity after polymerization. Curr. Protoc. Cell Biol. 47:10.16.1‐10.16.16. © 2010 by John Wiley & Sons, Inc.

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Multiphoton fabrication of chemically responsive protein hydrogels for microactuation

Cited by 184 publications

References 44 publications

Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Morphing in nature and beyond: a review of natural and synthetic shape-changing materials and mechanisms

Preparation of Hydrogel Substrates with Tunable Mechanical Properties

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