Multiphoton Excitation Fluorescence Microscopy and Spectroscopic Multianalytical Approach for Characterization of Historical Glass Grisailles

Oujja, M.; Agua, Fernando; Sanz, Mikel; Morales-Martín, Daniel; Garcı́a-Heras, Manuel; Villegas, M.; Castillejo, Marta

doi:10.1016/j.talanta.2021.122314

Cited by 13 publications

(21 citation statements)

References 48 publications

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“…For MPEF studies, we used a home-made nonlinear optical microscope, described in detail elsewhere [37,38,44,45], based on a mode-locked Ti:Sapphire femtosecond laser source. Briefly, the femtosecond laser emitting at 800 nm with an average power of 640 mW, pulse duration of 75 fs and a repetition rate of 80 MHz, was modulated by a chopper at a frequency of 130 Hz.…”

Section: Methodsmentioning

confidence: 99%

“…The thickness value of the glass alteration layer was obtained by recording MPEF z-scan profiles in six different points of the sample surface. The full width at half maximum (FWHM) resulting from a Lorentzian function fitting of the profiles was then averaged and corrected by the apparent depth correction factor [37,38]…”

Section: Methodsmentioning

confidence: 99%

“…laser-induced breakdown spectroscopy (LIBS) and laser-induced fluorescence (LIF) are complementary techniques for the characterization of historical glasses. LIBS is micro-invasive and capable to determine the chemical elemental composition of historical glasses and alteration layers with stratigraphic capability [33][34][35][36][37]. On the other hand, LIF provides non-invasive superficial analytical information and facilitates the detection of trace elements and/or chromophores responsible of glass coloration [33,38].…”

Section: Introductionmentioning

confidence: 99%

“…In the last years, cutting edge nonlinear optical microscopies (NLOM), using femtosecond lasers as light excitation sources, have been implemented in the field of cultural heritage as advanced tools for non-invasive 3D structural and chemical analysis [37,38,[42][43][44][45][46][47][48]. As compared to traditional microscopy, NLOM offers several advantages associated with the intrinsic high-resolution three-dimensional sectioning capability.…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, MPEF provides 3D information with micrometric axial and lateral resolution on the chemical and structural composition of the sample [38,[42][43][44][45][46][47]49]. In the case of historical glasses, MPEF has been successfully applied for non-invasive characterization of surface decorations (grisailles) on historical stained glass windows [37].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of medieval-like glass alteration layers by laser spectroscopy and nonlinear optical microscopy

et al. 2021

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Historical glass-based objects undergo, since the time of their manufacture, different degradation phenomena that are related to their composition and to the environment to which they were exposed. Three-dimensional (3D) structural and chemical characterization of the degradation layers is important to select the most adequate conservation strategies for glass objects. Optical microscopy (OM) is the most frequently used non-destructive method to examine the surface of historical glasses; however, the 3D structural assessment of alteration layers requires applying the destructive modality of this technique to conduct a cross-sectional study. In this work, a different approach for structural and compositional characterization of alteration layers on model medieval-like glasses is presented, based on the combination of the laser spectroscopies of laser-induced breakdown spectroscopy (LIBS), laser-induced fluorescence (LIF) and FT-Raman, and the emerging, cutting edge technique of nonlinear optical microscopy (NLOM) in the modality of multiphoton excitation fluorescence (MPEF). The results obtained through this multi-analytical photonic approach were compared with those retrieved by examination of the surface and cross sections of the samples by OM and scanning electron microscopy–energy-dispersive X-ray spectroscopy (SEM–EDS). While the combination of LIBS, LIF and FT-Raman served to assess the composition of the various alteration layers, the use of MPEF microscopy allowed the non-destructive determination of the thicknesses of these layers, showing for both thickness and composition a good agreement with the OM and SEM–EDS results. Thus, the proposed approach, which avoids sample preparation, illustrates the capability of non-destructive, or micro-destructive in the case of LIBS, laser spectroscopies and microscopies for the in situ study of glass objects of historic or/and artistic value. Graphic Abstract

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of medieval-like glass alteration layers by laser spectroscopy and nonlinear optical microscopy

et al. 2021

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Multi‐color two‐photon scanning structured illumination microscopy imaging of live cells

Wang

Chen

et al. 2023

Journal of Biophotonics

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Multi‐color two‐photon microscopy imaging of live cells is essential in biology. However, the limited diffraction resolution of conventional two‐photon microscopy restricts its application to subcellular organelle imaging. Recently, we developed a laser scanning two‐photon non‐linear structured illumination microscope (2P‐NLSIM), whose resolution improved three‐fold. However, its ability to image polychromatic live cells under low excitation power has not been verified. Here, to improve the reconstruction super‐resolution image quality under low excitation power, we increased the image modulation depth by multiplying the raw images with the reference fringe patterns in the reconstruction process. Simultaneously, we optimized the 2P‐NLSIM system to image live cells, including the excitation power, imaging speed, and field of view. The proposed system could provide a new imaging tool for live cells.

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Emerging photonic technologies for cultural heritage studies: the examples of non-linear optical microscopy and photoacoustic imaging

et al. 2022

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The availability of non-invasive technologies, which can be used separately or in combination for obtaining chemical composition data and structural information of Cultural Heritage (CH) materials, is of prime importance for improving the understanding the environmental or ageing impact on monuments and artefacts and defining optimal strategies for their conservation. This paper overviews and assesses the potential of two emerging photonic technologies, the Non-linear Optical Microscopy (NLOM) and Photoacoustic (PA) imaging modalities, for a variety of diagnostic applications in preservation science. These techniques, which are well-established in biomedical research, during the last few years have been also investigated as non-invasive tools for the in-depth, high-resolution analysis of various CH objects, including paintings, documents and murals. We discuss on the applicability of these diagnostic optical methods to obtain precise stratigraphic information in artefacts, evaluating additionally the presence and the extent of potential morphological or chemical changes in several CH materials due to ageing. Furthermore, we demonstrate that the contrast complementarity of NLOM and PA imaging provides invaluable insights into the structural integrity of an artwork, which can be subsequently utilized for the early and accurate detection of depth degradation effects.

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Multiphoton Excitation Fluorescence Microscopy and Spectroscopic Multianalytical Approach for Characterization of Historical Glass Grisailles

Cited by 13 publications

References 48 publications

Characterization of medieval-like glass alteration layers by laser spectroscopy and nonlinear optical microscopy

Characterization of medieval-like glass alteration layers by laser spectroscopy and nonlinear optical microscopy

Multi‐color two‐photon scanning structured illumination microscopy imaging of live cells

Emerging photonic technologies for cultural heritage studies: the examples of non-linear optical microscopy and photoacoustic imaging

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