Multiphasic uptake of D-glucose by an oligotrophic marine bacterium

Nissen, Hilde; Nissen, P.; Azam, F.

doi:10.3354/meps016155

Cited by 72 publications

(50 citation statements)

References 18 publications

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“…The multiphase uptake kinetics in oligotrophic marine bacteria was not shown to be attributable to a single transporter (17,18). However, it is tempting to speculate, as Muñoz-Marín et al…”

Section: Multiphasic Uptake Kinetics For Glucosementioning

confidence: 81%

“…Interestingly, the Pro1404-encoded sugar transporter exhibited multiphasic uptake kinetics, i.e., having low-affinity and high-affinity phases. This has not been observed before for sugar transport in cyanobacteria but has been observed in other oligotrophic marine bacteria (17,18) characterized the uptake kinetics for Prochlorococcus SS120 and found that it also exhibited multiphasic kinetics. Furthermore, they estimated the MichaelisMenton affinity constants and found that the high-affinity K s value (123 nM) and the low-affinity K s (0.9 μM) were the same for uptake in cultures of Prochlorococcus SS120 and for the Pro1404-encoded transporter in the recombinant S. elongatus PCC7942, adding further support to the role of Pro1404 gene in glucose uptake.…”

Section: Multiphasic Uptake Kinetics For Glucosementioning

confidence: 83%

“…(1) do, that having a multiphasic transporter in oligotrophic marine microbes makes sense in an environment that has low concentrations of dissolved organic material with occasional, localized spikes (18,22), so that the cell can rapidly adapt and not spend energy making new proteins. One might expect this strategy to be widespread in the minimal genome of Prochlorococcus (23).…”

Section: Multiphasic Uptake Kinetics For Glucosementioning

confidence: 99%

See 2 more Smart Citations

More mixotrophy in the marine microbial mix

Moore

2013

Proc. Natl. Acad. Sci. U.S.A.

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Section: Multiphasic Uptake Kinetics For Glucosementioning

confidence: 81%

Section: Multiphasic Uptake Kinetics For Glucosementioning

confidence: 83%

Section: Multiphasic Uptake Kinetics For Glucosementioning

confidence: 99%

See 1 more Smart Citation

More mixotrophy in the marine microbial mix

Moore

2013

Proc. Natl. Acad. Sci. U.S.A.

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“…The existence of a multiphasic uptake system for glucose has been shown for a bacterium isolated from oligotrophic seawater (Nissen et al 1984). The isolate was able to take up glucose at concentrations varying from 10 -9 to 10 -3 mol l -l .…”

Section: Dms Production In Response To Dmsp D Pulses: the Linear Respmentioning

confidence: 92%

Dynamics of dimethylsulfide production from dissolved dimethylsulfoniopropionate in the Labrador Sea

Schultes¹,

Levasseur²,

Michaud³

et al. 2000

Mar. Ecol. Prog. Ser.

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) throughout the study area. Causal links established with path analysis indicate that this potential seemed to be controlled by water temperature and chlorophyll a concentrations. 202: 27-40, 2000 of sulfur (Lovelock et al. 1972, Andreae 1990, Bates et al. 1992. KEY WORDS: Dimethylsulfide (DMS) · Dimethylsulfoniopropionate (DMSP) · Production rates · Low temperature · Bacteria · Substrate utilization Resale or republication not permitted without written consent of the publisherMar Ecol Prog SerThe main precursor of DMS is dimethylsulfoniopropionate (DMSP, [CH 3 ] 2 S + CH 2 CH 2 COO -), an osmolyte produced by certain groups of macro-and microalgae, similar in structure to the betaines (Vairavamurthy et al. 1985, Blunden & Gordon 1986, see Kirst 1996and Malin & Kirst 1997 for a review). Among phytoplankton, dinoflagellates and prymnesiophytes are known to possess high intracellular DMSP (Keller et al. 1989). Release of DMSP into the water during the exponential growth phase of the algae, i.e. exudation, is considered to be small (Laroche et al. 1999). The formation of an important pool of dissolved DMSP (DMSP d ) in marine surface waters is associated with cell senescence at the end of a bloom (Turner et al. 1988, Matrai & Keller 1993 especially since autolysis of phytoplankton seems to be an important process in the natural environment (Van Boekel et al. 1992, Brussaard et al. 1995. Liberation of DMSP d can furthermore be concomitant with DMS production via cell lysis due to zooplankton grazing (Dacey & Wakeham 1986, Leck et al. 1990) and viral infections (Malin et al. 1994, Bratbak et al. 1995, Hill et al. 1998. In open ocean regions, DMSP d concentrations as high as 200 nmol l -1 have been observed (Malin et al. 1993). In the water column, DMSP d is subject to rapid turnover. A part of the DMSP d undergoes enzymatic cleavage forming DMS and acrylic acid in equimolar amounts (Challenger & Simpson 1948, Cantoni & Anderson 1956). Activity of the enzymes (DMSPlyases) catalyzing the cleavage of DMSP has been observed and characterized in certain algae, for example Phaeocystis sp. or Emiliania huxleyi (Stefels & van Boekel 1993, Stefels & Dijkhuizen 1996, Steinke et al. 1998, and in bacteria from various aquatic environments (de Souza & Yoch 1995a, Taylor & Visscher 1996 and references therein). Bacterial cleavage of DMSP d to DMS and acrylic acid seems to be motivated by the use of the latter as a carbon source (Dacey & Blough 1987, Ledyard 1993. By incubating whole surface water communities with additions of DMSP d , it has been shown that enzymatic conversion of DMSP d to DMS is concentration and temperature dependent (Kiene & Service 1991), and follows Michaelis Menten type kinetics (Ledyard 1993). In natural waters, the kinetic parameters K m and V max of DMSP d consumption and DMS production show as yet unexplained variability that could be due to differences in microbial communities on seasonal or spatial scales (Ledyard 1993, Ledyard & Dacey 1996a.Bacterial demethylation of DMSP d to 3-methylmercaptopr...

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“…D'autre part, au niveau de la cellule bactérienne, le processus de limitation nutritive implique des changements séquentiels d'activités mé-taboliques, physiologiques et ultrastructuraux. De tels changements peuvent conduire à une réduction de la taille avec la production de minicellules (Morita 1985), à un changement dans les valeurs des constantes d'affinité (Km) et de vitesse maximale (Vmax) d'utilisation des substrats (Nissen et al 1984, Talbot & Bianchi 1997 et enfin, à des changements d'état physiologique de la croissance vers la mortalité, la latence ou la survie (Fig. 3).…”

Section: Introductionunclassified

Nouvelles approches d'étude des réseaux microbiens

Bianchi

1998

Ann. Limnol. - Int. J. Lim.

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Multiphasic uptake of D-glucose by an oligotrophic marine bacterium

Cited by 72 publications

References 18 publications

More mixotrophy in the marine microbial mix

More mixotrophy in the marine microbial mix

Dynamics of dimethylsulfide production from dissolved dimethylsulfoniopropionate in the Labrador Sea

Nouvelles approches d'étude des réseaux microbiens

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