Multiphase optofluidics on an electro-microfluidic platform powered by electrowetting and dielectrophoresis

Fan, Shih-Kang; Wang, Fu Min

doi:10.1039/c4lc00317a

Cited by 20 publications

(12 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2A). Considering that bead-based immunoassays take advantage of a large reaction surface area, a small sample quantity, and the simultaneous determination of multiple pathogens2829, we further expanded the platform to conduct simultaneous and quantitative monitoring of the growth of co-cultured bacteria. Pathogen-specific antibodies and fluorescent particles were combined to probe different pathogens (Fig.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform

Chung

Wang

Chuang

2017

Sci Rep

View full text Add to dashboard Cite

Successful treatments against bacterial infections depend on antimicrobial susceptibility testing (AST). However, conventional AST requires more than 24 h to obtain an outcome, thereby contributing to high patient mortality. An antibiotic therapy based on experiences is therefore necessary for saving lives and escalating the emergence of multidrug-resistant pathogens. Accordingly, a fast and effective drug screen is necessary for the appropriate administration of antibiotics. The mixed pathogenic nature of infectious diseases emphasizes the need to develop an assay system for polymicrobial infections. On this basis, we present a novel technique for simultaneous and quantitative monitoring of co-cultured microorganisms by coupling optical diffusometry with bead-based immunoassays. This simple integration simultaneously achieves a rapid AST analysis for two pathogens. Triple color particles were simultaneously recorded and subsequently analyzed by functionalizing different fluorescent color particles with dissimilar pathogen-specific antibodies. Results suggested that the effect of the antibiotic, gentamicin, on co-cultured Pseudomonas aeruginosa and Staphylococcus aureus was effectively distinguished by the proposed technique. This study revealed a multiplexed and time-saving (within 2 h) platform with a small sample volume (~0.5 μL) and a low initial bacterial count (50 CFU per droplet, ~105 CFU/mL) for continuously monitoring the growth of co-cultured microorganisms. This technique provides insights into timely therapies against polymicrobial diseases in the near future.

show abstract

Section: Resultsmentioning

confidence: 99%

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform

Chung

Wang

Chuang

2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…DMF can provide an effective way to improve the traditional DNA extraction because of its great flexibility and small quantity of reagent consumption. Moreover, the DMF can eliminate a requirement of complicated fluidic actuation components; this approach attracts much attention as a promising Lab-on-a-Chip platform that is suitable for chemical or biomedical applications, such as genetic testing or even NGS (Jebrail et al 2012;Kim et al 2013;Shamsi et al 2014;Fan and Wang 2014;Jebrail and Wheeler 2010;Hua et al 2010;Shen et al 2014;Hsieh et al 2014). Electrowetting-on-dielectric (EWOD) is a technique that adapts the DMF technique for the manipulation of micro droplets; it utilizes basically the change in the contact angle of a droplet placed over a dielectric substrate; EWOD with magnetic beads (M.B.)…”

Section: Introductionmentioning

confidence: 99%

A medical innovation: a new and improved method of DNA extraction with electrowetting-on-dielectric of genetic testing in-vitro fertilization (IVF)

Chiang

Huang

Lin

et al. 2020

Microfluid Nanofluid

View full text Add to dashboard Cite

The current technique for the detection of fetus genotype by in-vitro fertilization (IVF) is quite expensive and is unsuitable/ inconvenient for all patients and even affects the embryonic growth cycle. We thus propose a new method to extract small amount of cell-free DNA (cf-DNA) from embryo culture medium. We introduce a new format for DNA extraction depending on digital microfluidics (DMF), electrowetting-on-dielectric (EWOD) and magnetic forces to separate, suspend DNA-coated magnetic particles. Nano-droplets (of nano-liter size) are electrostatically controlled on an array of insulated electrodes by EWOD system. By applying voltage to the various electrodes, multiple nano-droplets can be manipulated precisely in the same device. This feature enables one to extract DNA at ultra-small concentrations (fg/μL to pg/μL) from an embryo culture medium with an EWOD system. We applied our EWOD platform to extract a small amount of cf-DNA from a clinical embryo culture medium of mouse. In the future, we will distinguish the DNA in the real embryo buffer according to the length of the telomere. This feature would be valuable for the selection of an embryo before an implantation when the gene information can be recognized.

show abstract

“…Microfluidic devices offer a wide variety of methods for handling small amounts of liquids in a controlled way. The so-called electromicrofluidic (EMF) method, operating by changing the interfacial properties of the driven liquids using electric forces, e.g., electrowetting-on-dielectric (EWOD) and dielectrophoresis (DEP), is one of the most popular and successful procedures to generate and move droplets on an array of electrostatically actuated electrodes [14][15][16][17][18][19]. EMF offers precise and programmable liquid positioning and is more reliable than other microfluidics procedures.…”

Section: Introductionmentioning

confidence: 99%

Optofluidic liquid sensing on electromicrofluidic devices

Oliva-Ramirez

Wang

Rico

et al. 2020

Mater. Res. Express

Self Cite

View full text Add to dashboard Cite

Electromicrofluidic (EMF) devices are used to handle and move tiny amounts of liquids by electrical actuation, including electrowetting-on-dielectric (EWOD) and dielectrophoresis (DEP). Monitoring the liquid characteristics in one of these devices requires suitable sensing transducers incorporated within the microfluidic structure. In the present work, we describe the incorporation of an optofluidic photonic transducer in an EMF device to monitor the refractive index of a liquid during its manipulation. The incorporated transducer consists of a responsive porous Bragg Microcavity (BM) deposited via physical vapor oblique angle deposition. Besides reporting the manufacturing procedure of the sensing-EMF device combining liquid handling and monitoring, the performance of the BM is verified by infiltrating several liquids dripped on its surface and comparing the responses with those of liquid droplets electrically moved from the delivery part of the chip to the BM location. This study proved that modified EMF devices can incorporate photonic structures to analyze very low liquid volumes (∼0.2 μL) during its handling.

show abstract

Multiphase optofluidics on an electro-microfluidic platform powered by electrowetting and dielectrophoresis

Abstract: Optofluidics on an electro-microfluidic platform by exploiting materials in the four fundamental phases – solid, liquid, gas, and plasma-phases – is reviewed.

Cited by 20 publications

References 49 publications

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform

A medical innovation: a new and improved method of DNA extraction with electrowetting-on-dielectric of genetic testing in-vitro fertilization (IVF)

Optofluidic liquid sensing on electromicrofluidic devices

Contact Info

Product

Resources

About