Multiphase flow modeling in centrifugal partition chromatography

Adelmann, S.; Schwienheer, C.; Schembecker, Gerhard

doi:10.1016/j.chroma.2011.01.063

Cited by 22 publications

(18 citation statements)

References 11 publications

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“…It is noteworthy that the stationary phase retention is column-specic (type, design and size). For example, the EtOAc-rich phase shows good retention in centrifugal partition chromatographic columns 49 and hence should not be excluded as a potential solvent for extracting astaxanthin from algal broth.…”

Section: Solvent Selectionmentioning

confidence: 99%

Direct extraction of astaxanthin from the microalgae Haematococcus pluvialis using liquid–liquid chromatography

Bauer

Minceva

2019

RSC Adv.

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Section: Solvent Selectionmentioning

confidence: 99%

Direct extraction of astaxanthin from the microalgae Haematococcus pluvialis using liquid–liquid chromatography

Bauer

Minceva

2019

RSC Adv.

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“…Schembecker at Dortmund (Germany) also used flow visualization to study flow patterns in a transparent disk CPC working with various solvent systems and comparing different cell designs. This group pointed out the impact of phase viscosity on stationary phase retention [10][11][12]. While suggesting improvements on cell design and a preferential selection of solvent systems with low interfacial tension, their advice on operating parameters is limited to the use of maximal rotation speed.…”

Section: Introductionmentioning

confidence: 99%

Effect of operating parameters on a centrifugal partition chromatography separation

Fumat

Berthod

Faure

2016

Journal of Chromatography A

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Centrifugal partition chromatography (CPC) is the branch of countercurrent chromatography (CCC) that works with single axis hydrostatic columns with rotary seals. The hydrodynamic of the liquid stationary phase-liquid mobile phase equilibrium in the CPC chambers has been studied theoretically and with specially designed CPC columns. In this work, we selected a simple analytical separation (no loading study) of three test solutes, coccine red, coumarin and carvone, with a commonly used heptane/ethyl acetate/methanol/water 1:1:1:1v/v biphasic liquid system and two different rotors: a commercially available 30-mL CPC instrument and a 80-mL prototype rotor designed for productivity. We fully studied this separation in many possible practical operating conditions of the two rotors, aiming at a generic column characterization. The rotor rotation was varied between 1000 and 2800rpm, the aqueous mobile phase flow rate was varied between 1 and 22mL/min with the 30-mL rotor and 10 and 55mL/min with the 80-mL rotor, the upper limits being mechanical constraints and some liquid stationary phase remaining in the rotor. The variations of Sf, the volume ratio of stationary phase in the rotor, were studied versus mobile phase flow rate and rotor rotation speed. A maximum mobile phase linear velocity was found to depend on the centrifugal field for the 30-mL rotor. This maximum velocity was not observed with the 80-mL rotor. Studying the changes in coumarin and carvone peak efficiencies, it is established that the number of cells required to make one theoretical plate, i.e. one chromatographic exchange, is minimized at maximal rotation speed and, to a lesser extent, at high mobile phase flow rate (or linear velocity). Considering the throughput, there is evidence of an optimal flow rate depending on the rotor rotation that is not necessarily the highest possible.

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“…Such estimation of specific interfacial area was not yet available as only the volumetric mass transfer coefficient, k L a, had been studied in the literature. In the volumetric mass transfer coefficient, the specific interfacial area, a, is not uncoupled from the mass transfer coefficient, k L [8,23] while only the value of the specific interfacial area is of importance for operation of interface catalyzed enzymatic reactions because it is directly related to the amount of effective biocatalyst in the system. Fig.…”

Section: Resultsmentioning

confidence: 99%

“…As a comparison Adelmann et al evaluated the interfacial area of a CPC device in various solvent systems [23]. They reported a value of the specific interfacial area, referred to the total CPR cell volume, between one and two thousands of m 2 .…”

Section: Determination Of Specific Interfacial Area In Continuous Opementioning

confidence: 99%

Lipase esterification in the Centrifugal Partition Reactor: Modelling and determination of the specific interfacial area

Nioi

Destrac

Condoret

2019

Biochemical Engineering Journal

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Multiphase flow modeling in centrifugal partition chromatography

Cited by 22 publications

References 11 publications

Direct extraction of astaxanthin from the microalgae Haematococcus pluvialis using liquid–liquid chromatography

Direct extraction of astaxanthin from the microalgae Haematococcus pluvialis using liquid–liquid chromatography

Effect of operating parameters on a centrifugal partition chromatography separation

Lipase esterification in the Centrifugal Partition Reactor: Modelling and determination of the specific interfacial area

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