Multiparametric quantitative phase imaging for real-time, single cell, drug screening in breast cancer

Polanco, Edward R.; Moustafa, Tarek E.; Butterfield, Andrew; Scherer, Sandra D.; Cortes-Sanchez, Emilio; Bodily, Tyler; Welm, Bryan E.; Bernard, Philip S.; Zangle, Thomas A.

doi:10.1101/2021.11.26.467625

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…To assess whether Aqp1 expression and the ensuing increase in water diffusivity is a safe approach for imaging cells, we compared Aqp1-expressing cells to those expressing GFP in terms of dry mass, volume, and sphericity (viz. roundedness), key morphological descriptors that are often altered by adverse changes in cell physiology [40][41][42][43][44][45][46] .…”

Section: Aqp1 Expression Does Not Alter Cell Mass Volume Sphericity A...mentioning

confidence: 99%

Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types

Miller,

Chowdhury,

Hanson

et al. 2023

Preprint

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Aquaporin-1 (Aqp1), a water channel, has garnered significant interest for cell-based medicine and in vivo synthetic biology due to its ability to be genetically encoded to produce magnetic resonance signals by increasing the rate of water diffusion in cells. However, concerns regarding the effects of Aqp1 overexpression and increased membrane diffusivity on cell physiology have limited its widespread use as a deep-tissue reporter. In this study, we present evidence that Aqp1 generates strong diffusion-based magnetic resonance signals without adversely affecting cell viability or morphology in diverse cell lines derived from mice and humans. Our findings indicate that Aqp1 overexpression does not induce ER stress, which is frequently associated with heterologous expression of membrane proteins. Furthermore, we observed that Aqp1 expression had no detrimental effects on native biological activities, such as phagocytosis, immune response, insulin secretion, and tumor cell migration in the analyzed cell lines. These findings should serve to alleviate any lingering safety concerns regarding the utilization of Aqp1 as a genetic reporter and should foster its broader application as a noninvasive reporter for in vivo studies.

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Section: Aqp1 Expression Does Not Alter Cell Mass Volume Sphericity A...mentioning

confidence: 99%

Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types

Miller,

Chowdhury,

Hanson

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Quantitative Phase Imaging: Recent Advances and Expanding Potential in Biomedicine

et al. 2022

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Quantitative phase imaging (QPI) is a label-free, wide-field microscopy approach with significant opportunities for biomedical applications. QPI uses the natural phase shift of light as it passes through a transparent object, such as a mammalian cell, to quantify biomass distribution and spatial and temporal changes in biomass. Reported in cell studies more than 60 years ago, ongoing advances in QPI hardware and software are leading to numerous applications in biology, with a dramatic expansion in utility over the past two decades. Today, investigations of cell size, morphology, behavior, cellular viscoelasticity, drug efficacy, biomass accumulation and turnover, and transport mechanics are supporting studies of development, physiology, neural activity, cancer, and additional physiological processes and diseases. Here, we review the field of QPI in biology starting with underlying principles, followed by a discussion of technical approaches currently available or being developed, and end with an examination of the breadth of applications in use or under development. We comment on strengths and shortcomings for the deployment of QPI in key biomedical contexts and conclude with emerging challenges and opportunities based on combining QPI with other methodologies that expand the scope and utility of QPI even further.

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Multiparametric quantitative phase imaging for real-time, single cell, drug screening in breast cancer

Cited by 2 publications

References 40 publications

Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types

Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types

Quantitative Phase Imaging: Recent Advances and Expanding Potential in Biomedicine

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