This paper describes the development of a cloth-based CL strategy that can quantify H 2 O 2 or glucose in a low-cost, easy-to-use, rapid and sensitive manner. Wax screen-printing is applied to fabricate the ultralow-cost cloth-based devices, and thus the as-prepared sensors have a very low cost (about $0.01 per assay). Moreover, an inexpensive CCD camera is used to image the light from the gravity/capillary flow-triggered enzyme-catalysed oxidation of luminol by H 2 O 2 . Under optimized conditions, the developed CL platform can fulfill the determination of H 2 O 2 , with a linear range of 0.01-10 mM and a detection limit of 9.07 mM. Similarly, glucose can be detected in the range of 0.01-10 mM, with a detection limit of 9.74 mM. Additionally, the RSDs of these two assays are 3.36%, 3.66% and 2.64% for 0.01, 0.1 and 5 mM H 2 O 2 , and 3.01%, 2.80% and 3.68% for 0.1, 1 and 5 mM glucose, respectively.Moreover, the devices can maintain $95% of their initial response for three weeks, and tolerate a series of interfering species at reasonable concentrations. Therefore, the proposed platform has an acceptable sensitivity, linear range, reproducibility and stability. Finally, this platform is successfully applied to the detection of H 2 O 2 in milk and glucose in human serum.