Multiorgan Metabolomics and Lipidomics Provide New Insights Into Fat Infiltration in the Liver, Muscle Wasting, and Liver–Muscle Crosstalk Following Burn Injury

Mert, Safak; Bulutoglu, Beyza; Chu, Christopher; Dylewski, Maggie L.; Lin, Florence; Yu, Yong‐Ming; Yarmush, Martin L.; Sheridan, Robert L.; Uygun, Korkut

doi:10.1093/jbcr/iraa145

Cited by 8 publications

(10 citation statements)

References 158 publications

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“…Conversely ALT, BUN, SCr and UA were positively raised with increasing severity of SII. Elevated ALT in the early phase of injury can be due to shock or hypovolemia, resulting in ischemia and hypoxia in the liver, and leading to liver damage ( 46 ). Reduced effective circulating blood volume due to various causes after burns leads to reduced renal blood flow and decreased glomerular filtration rate, resulting in increased BUN, SCr and UA stasis ( 47 ).…”

Section: Discussionmentioning

confidence: 99%

Clinical characteristics and predictors of burn complicated with smoke inhalation injury: A retrospective analysis

et al. 2022

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Fire smoke enters the human lungs through the respiratory tract. The damage to the respiratory tract and lung tissue is known as smoke inhalation injury (SII). Fire smoke can irritate airway epithelium cells, weaken endothelial cell adhesion and lyse alveolar type II epithelia cells, leading to emphysema, decreased lung function, pneumonia and risk of acute lung injury/acute respiratory distress syndrome (ARDS). The purpose of the present study was to analyze the clinical characteristics of patients with SII and the risk factors affecting their prognosis. A total of 103 patients with SII admitted between January 2016 to December 2021 to the Burns Unit of the Characteristic Medical Center of Chinese People's Armed Police Force and 983 Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army were selected for the present study. The demographics and clinical features between different severities of SII were analyzed. Univariate/multivariate logistic regression was used to analyze the potential predictors for severity, ARDS and mortality of patients with SII. Receiver operating characteristic (ROC) curves were used to screen independent risk factors and identify their prediction accuracy. It was concluded that total body surface area (TBSA), III burn area (of total %TBSA), cases of respiratory infections, ARDS morbidity, mortality, acute physiology and chronic health evaluation II, lung injury prediction score, lactic acid, white blood cells (WBC), alanine transaminase, blood urea nitrogen, serum creatinine and uric acid were indicators that were raised with increasing severity of SII. However red blood cells, hemoglobin, platelet count, total protein, albumin, and albumin/globulin were decreased with the increasing severity of SII (P<0.05). WBC >20.91 (10 9 /l) was a reliable indicator for severe SII. Lactic acid >9.60 (mmol/l) demonstrated a high degree of accuracy in predicting ARDS development in patients with SII. Hemoglobin <83.00 (g/l) showed a high degree of accuracy in predicting mortality. In summary, the highlighted assessment parameters could be used to contribute to devising improved treatment plans to preempt worsening conditions (such as shock, ARDS, multiple organ dysfunction syndrome and death).

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Section: Discussionmentioning

confidence: 99%

Clinical characteristics and predictors of burn complicated with smoke inhalation injury: A retrospective analysis

et al. 2022

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“…This includes observations of increased levels of free fatty acids (FFAs) in the plasma hypothesized to result from triacylglyceride (TAG) breakdown in adipose and muscle, , as well as acyl-carnitines in muscle tissue itself . In addition, accumulation of TAGs and lipoapoptotic-inducing palmitic and stearic lipids have been demonstrated to promote hepatic steatosis in the liver postburn injury. Conversely a significant decline in FFAs, monoacylglycerides (MAGs), lysophosphatidylglycerols (LPGs) and lysophosphatidylethanolamines (LPEs) has been noted in burn-affected skin …”

Section: Introductionmentioning

confidence: 99%

Nonsevere Burn Induces a Prolonged Systemic Metabolic Phenotype Indicative of a Persistent Inflammatory Response Postinjury

Ryan,

Raby,

Whiley

et al. 2023

J. Proteome Res.

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Globally, burns are a significant cause of injury that can cause substantial acute trauma as well as lead to increased incidence of chronic comorbidity and disease. To date, research has primarily focused on the systemic response to severe injury, with little in the literature reported on the impact of nonsevere injuries (<15% total burn surface area; TBSA). To elucidate the metabolic consequences of a nonsevere burn injury, longitudinal plasma was collected from adults (n = 35) who presented at hospital with a nonsevere burn injury at admission, and at 6 week follow up. A cross-sectional baseline sample was also collected from nonburn control participants (n = 14). Samples underwent multiplatform metabolic phenotyping using 1 H nuclear magnetic resonance spectroscopy and liquid chromatographymass spectrometry to quantify 112 lipoprotein and glycoprotein signatures and 852 lipid species from across 20 subclasses. Multivariate data modeling (orthogonal projections to latent structures-discriminate analysis; OPLS-DA) revealed alterations in lipoprotein and lipid metabolism when comparing the baseline control to hospital admission samples, with the phenotypic signature found to be sustained at follow up. Univariate (Mann−Whitney U) testing and OPLS-DA indicated specific increases in GlycB (p-value < 1.0e −4 ), low density lipoprotein-2 subfractions (variable importance in projection score; VIP > 6.83e −1 ) and monoacyglyceride (20:4) (p-value < 1.0e −4 ) and decreases in circulating anti-inflammatory high-density lipoprotein-4 subfractions (VIP > 7.75e −1 ), phosphatidylcholines, phosphatidylglycerols, phosphatidylinositols, and phosphatidylserines. The results indicate a persistent systemic metabolic phenotype that occurs even in cases of a nonsevere burn injury. The phenotype is indicative of an acute inflammatory profile that continues to be sustained postinjury, suggesting an impact on systems health beyond the site of injury. The phenotypes contained metabolic signatures consistent with chronic inflammatory states reported to have an elevated incidence postburn injury. Such phenotypic signatures may provide patient stratification opportunities, to identify individual responses to injury, personalize intervention strategies, and improve acute care, reducing the risk of chronic comorbidity.

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“…The liver, adipose tissue, skin and muscles are impacted during the hypermetabolic phase of a burn injury with several severe burn studies documenting changes in lipids. Increasing levels of FFAs have been shown in the plasma from triacylglyceride (TAG) breakdown in adipose and muscle 14,15 , as well as acyl-carnitines in muscle 16 . In addition, accumulation of TAGs 16 and lipoapoptoticinducing palmitic and stearic lipids 17 has been demonstrated to promote hepatic steatosis in the liver.…”

Section: Introductionmentioning

confidence: 99%

“…Increasing levels of FFAs have been shown in the plasma from triacylglyceride (TAG) breakdown in adipose and muscle 14,15 , as well as acyl-carnitines in muscle 16 . In addition, accumulation of TAGs 16 and lipoapoptoticinducing palmitic and stearic lipids 17 has been demonstrated to promote hepatic steatosis in the liver. Conversely a significant decline in FFAs, monoacylglycerides (MAGs), lysophosphatidylglycerols (LPGs) and lysophosphatidylethanolamines (LPEs) has been noted in burn-affected skin 18 .…”

Section: Introductionmentioning

confidence: 99%

Non-severe burns induce a prolonged systemic metabolic phenotype indicative of a persistent inflammatory response post-injury

Ryan

Raby

Masuda

et al. 2023

Preprint

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To understand the metabolic consequences following non-severe burns, longitudinal plasma was collected from adults with non-severe burn injuries (n=35) within 48 hours of admission to hospital and at 6 weeks post-surgery, as well as non-burn controls (n=14). Samples were analysed by 1H nuclear magnetic resonance spectroscopy and liquid chromatography-tandem mass spectrometry to quantify 112 lipoprotein, supramolecular phospholipid composite (SPC), GlycB arising from the glycoprotein N-acetylneuraminidino groups and 852 lipid species across 20 chemical subclasses. Alterations in lipoprotein and lipid metabolism were evident in samples taken within 48 hours of admission and found to be sustained at the 6-week timepoint, with increases in GlycB signal (p-value < 0.0001) and the multivariate importance of the apolipoprotein B100/apolipoprotein A1 (ABA1) ratio, as well as decreases in anti-inflammatory phosphatidylcholines, phosphatidylglycerols, phosphatidylinositols and phosphatidylserines. Low circulating high density lipoproteins, and increases in pro-atherogenic low density lipoproteins and monoacylglyceride (20:4) (p-value < 0.0001) are potentially indicative of an increased risk of cardiovascular disease after non-severe burns which requires further investigation.

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Multiorgan Metabolomics and Lipidomics Provide New Insights Into Fat Infiltration in the Liver, Muscle Wasting, and Liver–Muscle Crosstalk Following Burn Injury

Cited by 8 publications

References 158 publications

Clinical characteristics and predictors of burn complicated with smoke inhalation injury: A retrospective analysis

Clinical characteristics and predictors of burn complicated with smoke inhalation injury: A retrospective analysis

Nonsevere Burn Induces a Prolonged Systemic Metabolic Phenotype Indicative of a Persistent Inflammatory Response Postinjury

Non-severe burns induce a prolonged systemic metabolic phenotype indicative of a persistent inflammatory response post-injury

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