Multiomics analysis provides insights into alkali stress tolerance of sunflower (Helianthus annuus L.)

Lü, Huiying; Wang, Ziqi; Xu, Chenyang; Li, Luhao; Yang, Chunwu

doi:10.1016/j.plaphy.2021.05.032

Cited by 25 publications

(9 citation statements)

References 29 publications

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“…Usually, the stress induced by neutral salts NaCl and Na 2 SO 4 and alkaline salt Na 2 CO 3 and NaHCO 3 is called salt stress and alkaline stress. In contrast, mixed saline-alkaline stress is caused by both neutral and alkaline salt ( Lu et al, 2021 ). Although research on these topics has been conducted, most of them employed neutral salts to simulate saline-alkaline stress, and few studies have been carried out on alkaline salt stress.…”

Section: Introductionmentioning

confidence: 99%

Exogenous Melatonin Alleviates Alkaline Stress by Removing Reactive Oxygen Species and Promoting Antioxidant Defence in Rice Seedlings

Min

Shi

et al. 2022

Front. Plant Sci.

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Saline-alkali stress seriously restricts rice growth, development, and production in northern China. The damage of alkaline stress on rice is much greater than that of salt due to ion toxicity, osmotic stress, and especially high pH. As a signal molecule, melatonin (N-acetyl-5-methoxytryptamine, MT) mediates many physiological processes in rice and participates in protecting rice from abiotic stress. The potential mechanism of exogenous melatonin-mediated alkaline stress tolerance is still largely unknown. In this study, the effects of melatonin on the morphological change, physiological property, and corresponding genes expression in rice seedlings were analyzed under alkaline stress (20 mmol L−1, pH 9.55). The results showed that the expression levels of MT synthesis genes (TDC2, T5H, SNAT, ASMT1, and ASMT2) were induced by both exogenous MT and alkaline stress treatment. The cell membrane was protected by MT, and the MT furtherly play role in scavenging reactive oxygen species (ROS), reducing lipoxygenase (LOX) activity, and malondialdehyde (MDA) content. The scavenging of ROS by melatonin is attributed to the coupling of the improvement of redox homeostasis and the enhancement of antioxidant enzyme activity and antioxidant content by upregulating the transcriptional levels of antioxidase genes. In the meantime, MT pretreatment promoted the accumulation of free proline, sucrose, and fructose by regulating the OsP5CS, OsSUS7, and OsSPS1 gene expression level and increased chlorophyll content upregulating the expression of chlorophyll synthesis-related genes. Ultimately, the alleviating effect of exogenous melatonin on alkaline stress was reflected in increasing the leaf relative water content (RWC) and root-shoot ratio and reducing the leaf tip wilt index (TWI) through a series of physiological and biochemical changes. Melatonin pretreatment changed the expression level of MT synthesis genes which might contribute to MT synthesis in rice, consequently, activated the ROS scavenging system and alleviating the damage of alkaline stress on rice seedlings. Our study comprehensively understands the alleviating effect of exogenous melatonin on rice under alkaline stress.

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Section: Introductionmentioning

confidence: 99%

Exogenous Melatonin Alleviates Alkaline Stress by Removing Reactive Oxygen Species and Promoting Antioxidant Defence in Rice Seedlings

Min

Shi

et al. 2022

Front. Plant Sci.

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“…Neutral salts, such as NaCl and Na 2 SO 4 , and alkaline salts, such as NaHCO 3 and Na 2 CO 3 , are the primary ion sources found in saline soils; Na + , K + , Ca 2+ , and Mg 2+ are the main cations and C1 − , NO 3 − , HCO 3 − , CO 3 2− , and SO 4 2− are the main anions. Indeed, studies have confirmed that alkaline salts damage plants more than neutral salts (Guo et al, 2015;Wang et al, 2017;Zhang et al, 2020;Lu et al, 2021). Therefore, comparing the differential response of plants to neutral and alkaline salt stress is important for improving the utilization of saline-alkaline soils for agricultural production (Wang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Alkaline Salt Inhibits Seed Germination and Seedling Growth of Canola More Than Neutral Salt

et al. 2022

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Salinity is a major constraint to crop growth and productivity, limiting sustainable agriculture production. Planting canola (Brassica napus L.) variety with salinity-alkalinity tolerance as a green manure on the large area of salinity-affected land in Xinjiang could alleviate feed shortage. To investigate the differential effects of neutral and alkaline salt stress on seed germination and seedling growth of canola, we used two salts at varying concentrations, i.e., NaCl (neutral salt at 100, 150, and 200 mM) and Na2CO3 (alkaline salt at 20, 30, and 40 mM). To further explore the effects of Na+ and pH on seed germination, we included combined of NaCl (0, 100, 150, and 200 mM) and pH (7.1, 8.0, 9.0, 10.0, and 11.0). Shoot growth was promoted by low concentrations of NaCl and Na2CO3 but inhibited at high salt concentrations. Given the same Na+ concentration, Na2CO3 inhibited seed germination and seedling growth more than NaCl. The results showed that the main factor affecting seed germination and seedling growth is not pH alone, but the interaction between pH and salt ions. Under NaCl stress, canola increased the absorption of K+, Ca2+, and Mg2+ in roots and K+ in leaves. However, under Na2CO3 stress, canola maintained a high K+ concentration and K+/Na+ ratio in leaves and increased Ca2+ and Mg2+ in roots. Our study showed that alkaline salts inhibit canola seed germination and seedling growth more significantly than neutral salts and salt species, salt concentration, and pH significantly affected on seed germination and seedling growth. However, pH affected seed germination and seedling growth mainly through an interaction with salt ions.

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“…Under alkali stress, β-oxidation, glycolysis, citric acid cycle, and amino acid metabolism serve important roles in improving alkali tolerance ( Zhang et al, 2016 ; Xiao et al, 2020 ). Enhanced glycolysis process provided more carbon source and energy for alkali stress response of roots ( Lu et al, 2021 ). Meanwhile, Sucrose acted as a signaling molecule and directly mediated amino acid metabolism ( Jia et al, 2019 ), and other small-molecule soluble substances such as organic acids, amino acids, betaine, sugars, and alcohols are synthesized and accumulated ( Munns and Tester, 2008 ; Sun et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Ionomics and Metabolic Responses and Adaptive Strategies of Cotton to Salt and Alkali Stress

Guo

Tao

et al. 2022

Front. Plant Sci.

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Soil salinization and alkalization severely inhibit agriculture. However, the response mechanisms of cotton to salt stress or alkali stress are unclear. Ionomics and metabolomics were used to investigate salt and alkali stresses in cotton roots and leaves. Compared with the control, salt-treated and alkali-treated cotton plants showed 51.8 and 53.0% decreases in biomass, respectively. Under salt stress, the concentration of N decreased in roots but increased in leaves, and the concentrations of P and K increased in roots but decreased in leaves. Salt stress inhibited Ca, B, N, and Fe uptake and Mg, K, P, S, and Cu transport, but promoted Mo, Mn, Zn, Mg, K, P, S, and Cu uptake and Mo, Mn, Zn, B, N, and Fe transport. Under alkali stress, the concentrations of N and P in roots and leaves decreased, while the concentrations of K in roots and leaves increased. Alkali stress inhibited P, Ca, S, N, Fe, and Zn uptake and N, P, Mg and B transport, but promoted K, Mn, Cu, Mo, Mg, and B uptake and K, Mn, Cu, Mo, Fe, and Zn transport. Under salt stress in the leaves, 93 metabolites increased, mainly organic acids, amino acids, and sugars, increased in abundance, while 6 decreased. In the roots, 72 metabolites increased, mainly amino acids, organic acids, and sugars, while 18 decreased. Under alkali stress, in the leaves, 96 metabolites increased, including organic acids, amino acids, and sugars, 83 metabolites decreased, including organic acids, amino acids, and sugars; In the roots, 108 metabolites increased, including organic acids, amino acids, and sugars. 83 metabolites decreased, including organic acids and amino acids. Under salt stress, cotton adapts to osmotic stress through the accumulation of organic acids, amino acids and sugars, while under alkali stress, osmoregulation was achieved via inorganic ion accumulation. Under salt stress, significant metabolic pathways in the leaves and roots were associated with amino acid and organic acid metabolism, sugar metabolism was mainly used as a source of energy, while under alkali stress, the pathways in the leaves were related to amino acid and linoleic acid metabolism, β-Oxidation, TCA cycle, and glycolysis were enhanced to provide the energy needed for life activities. Enhancing organic acid accumulation and metabolism in the roots is the key response mechanism of cotton to alkalinity.

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Multiomics analysis provides insights into alkali stress tolerance of sunflower (Helianthus annuus L.)

Cited by 25 publications

References 29 publications

Exogenous Melatonin Alleviates Alkaline Stress by Removing Reactive Oxygen Species and Promoting Antioxidant Defence in Rice Seedlings

Exogenous Melatonin Alleviates Alkaline Stress by Removing Reactive Oxygen Species and Promoting Antioxidant Defence in Rice Seedlings

Alkaline Salt Inhibits Seed Germination and Seedling Growth of Canola More Than Neutral Salt

Comparative Ionomics and Metabolic Responses and Adaptive Strategies of Cotton to Salt and Alkali Stress

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