Multiomic analysis of dark tea extract on glycolipid metabolic disorders in db/db mice

Abstract

Glycolipid metabolic disorder is a serious threat to human health. Dark tea is a kind of traditional Chinese tea, which may regulate the glycolipid metabolic disorders. Dark tea extract (DTE) is the water extraction obtained from dark tea. Compared with traditional dark tea, DTE has the benefits of convenient consumption and greater potential for promoting health. However, the regulation of DTE on glycolipid metabolism and its molecular mechanism is rarely investigated. In our study, DTE was used as raw material to study the effect and molecular mechanism of its intervention on the glycolipid metabolic in db/db diabetic mice by using multiomics analysis and modern biological techniques. (1) DTE could significantly reduce fasting glucose in diabetic db/db mice, and the higher dose group has a better effect. Histopathological examination showed that DTE slightly improve the number of islets and decrease the number of islet β cells in the pancreatic tissue in db/db mice. (2) RNA-Seq was used to analyze the gene expression in liver tissue. In terms of biological processes, DTE mainly affected the inflammation and fatty acid metabolism. In terms of cell components, the lipoprotein and respiratory chain are mainly affected. In the aspect of molecular function, DTE mainly affected the redox related enzyme activity, iron ion binding and glutathione transferase. Arachidonic acid metabolism pathway, glutathione metabolism and PPAR signaling pathway were enriched by DTE with the results of KEGG pathway enrichment. In addition, real-time PCR results confirmed that DTE could significantly activate key genes of PPAR signaling pathway like Fabp1, Cyp4a1, Ehhadh, Cyp4a32, Aqp7 and Me1. (3) 16s rDNA showed that DTE could significantly decrease the ratio of Firmicutes/Bacteroidetes and the abundance of Proteobacteria, and increased the relative abundance of Verrucomicrobia at the phylum level. At the genus level, the relative abundance of Akkermansia, Prevotellaceae, Bacteroides and Alloprevotella was significantly increased after DTE treatment. This study provides multiomics molecular evidence for the intervention effect of DTE on abnormal glucose and lipid metabolism and the application of precise nutritional diet intervention of dark tea extract.

Tibetan tea reduces obesity brought on by a high‐fat diet and modulates gut flora in mice

“…Statistics show that each year, almost 2.8 million individuals pass away due to obesity‐related illnesses (Wiciński et al, 2020 ), making the need for obesity treatment and prevention urgent. According to several researches, drinking dark tea may help prevent and cure obesity (Lim et al, 2022 ; Wang, Hu, et al, 2022 ). There have been claims that the dark tea variety TT offers anti‐obesity properties (Yuan et al, 2016 ).…”

Bazi Bushen ameliorates age-related energy metabolism dysregulation by targeting the IL-17/TNF inflammatory pathway associated with SASP

“…In the BZBS-activated PPAR signaling pathway, genes such as Cyp4a32 , Cyp4a31 , Cyp4a14 , Cyp4a10 , and Plin4 were upregulated (Fig. 5 E), and these genes are involved in the regulation of glucose and lipid metabolism in db/db diabetic mice [ 49 ].

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A Rising Star in Dark Teas: The Processing, Microorganisms, Chemicals, Bioactivities, and Safety of Qingzhuan Tea

“…53 In the process of QZT fermentation, GA content also showed a decreasing trend due to similar reasons, and the degradation of GA may be an important reason for the unique aging flavor of QZT. In addition, Feng et al isolated 3-O-methylgallate (62), salicyluric acid (63), xanthurenic acid (64), niacin (65), adipic acid (66), 4-hydroxycinnamic acid (67), quinic acid (68), coumarin (69), salicylic acid (70), coumaric acid (71), 2,5-dihydroxybenzaldehyde (72), 4hydroxybenzoic acid (73), 3-hydroxycoumarin (74), 2-(acetyloxy)-benzoic acid (75), salicylaldehyde (76), caffeic acid (77), indoleacrylic acid (78), theogallin (79), and other metabolic compounds (Figure 6). 44 Cheng et al also found five other phenolic acid compounds from QZT, including 3,5-diOH-benzoic acid (80), m-coumaric acid (81), 5-G-quinic acid (82), 3-p-coumaroyl-quinic acid (83), and α-linolenic acid (84).…”

“…74 Meanwhile, QZT can effectively increase the insulin cells in pancreatic tissue and reduce the blood sugar level to regulate diabetes and a series of complications. Wang et al investigated the influence of the extract from QZT on diabetic mice 75 and found that the QZT extract reduced the blood sugar level of diabetic mice, which further repaired the damage of pancreatic organs and stimulated insulin secretion, thus, relieving diabetes-related symptoms. In another study, Feng et al studied the inhibitory effects of changes to metabolites on lipase and α-amylase during the pile-fermentation of QZT.…”