Multiobjective optimization of the antioxidant activities of horse mackerel hydrolysates produced with protease mixtures

Morales‐Medina, R.; Pérez‐Gálvez, Raúl; Guadix, Antonio; Guadix, Emilia M.

doi:10.1016/j.procbio.2016.11.001

Cited by 19 publications

(19 citation statements)

References 41 publications

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“…Higher capacity of hydrolysis of Esperase (30.7%) in comparison to Alcalase (26.3%) was predicted under the optimal conditions mentioned ( Table 2 ). Our H m data for Alcalase are in general similar or higher to other species as Yellow stripe trevally, Pacific whiting, Cape hake and horse mackerel [ 8 , 10 , 26 ]. However, greater degree of hydrolysis (44%) was described for salmon-FPH using an acid fungal protease [ 27 ] and anchovy sprat-FPH formulated with Papain or Bromelain [ 25 ].…”

Section: Resultssupporting

confidence: 77%

“…Lipid oxidation and cardiovascular diseases are of particular concern for the food industry and international public health organizations, respectively. FPH from other fish substrates and by-products have been cited to possess antihypertensive and antioxidant bioactivities [ 7 , 8 , 9 , 10 ]. Only one reference about the production of enzymatic hydrolysates with biological properties from S. canicula has been reported [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Production of Fish Protein Hydrolysates from Scyliorhinus canicula Discards with Antihypertensive and Antioxidant Activities by Enzymatic Hydrolysis and Mathematical Optimization Using Response Surface Methodology

et al. 2017

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Fish discards are of major concern in new EU policies. Alternatives for the management of the new biomass that has to be landed is compulsory. The production of bioactive compounds from fish protein hydrolysates (FPH) has been explored in recent years. However, the viability of Scyliorhinus canicula discards, which might account for up to 90–100% of captures in mixed trawler, gillnet, and longline industrial fisheries, to produce FPH from the muscle with bioactivities has still not been studied in terms of the optimization of the experimental conditions to enhance its production. The effect of pH and temperature on the hydrolysis of the S. canicula muscle was mediated by three commercial proteases using response surface methodology. Temperatures of 64.6 °C and 60.8 °C and pHs of 9.40 and 8.90 were established as the best hydrolysis conditions for Alcalase and Esperase, respectively. Optimization of the best conditions for the maximization of antihypertensive and antioxidant activities was performed. Higher Angiotensin-converting enzyme (ACE) activity was found with Esperase. The pH optimum and temperature optimum for antioxidants were 55 °C/pH8.0 for ABTS/DPPH-Esperase, 63.1 °C/pH9.0 for DPPH-Alcalase, and 55 °C/pH9.0 for ABTS-Alcalase. No hydrolysis was detected when using Protamex.

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Section: Resultssupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Production of Fish Protein Hydrolysates from Scyliorhinus canicula Discards with Antihypertensive and Antioxidant Activities by Enzymatic Hydrolysis and Mathematical Optimization Using Response Surface Methodology

et al. 2017

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“…Studies for optimisation of hydrolysates production conditions are important to improve the efficiency of these processes. Although there are many studies that address the production of common bean peptides, few studies that use the design of protease mixtures on the antioxidant peptides production were reported (De Castro & Sato, , ; Morales‐Medina et al ., ). This type of statistical evaluation can provide models and mixture contour plots that indicate the most suitable enzymatic mixtures to obtain hydrolysates with increased antioxidant activities (De Castro et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Improving antioxidant activity of black bean protein by hydrolysis with protease combinations

Aguilar

Cason

Castro

2018

Int J of Food Sci Tech

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This study explored the use of a simplex centroid design to produce protein hydrolysates with antioxidant properties using Alcalase Ò 2.4L, Flavourzyme Ò 500L and Neutrase Ò 0.8L. Proteases kinetic parameters and the ultrafiltration of protein hydrolysates were also investigated. The highest antioxidant activity, in the studied conditions, was reached when the mixture of Alcalase Ò 2.4L and Flavourzyme Ò 500L was used in the hydrolysates production. The antioxidant power of the black bean proteins, measured by the total antioxidant capacity and reducing power assay, increased after hydrolysis by 31% and 70%, respectively. The black bean proteins hydrolysates fractions (3-30 kDa) showed an antioxidant activity decrease along with a reduction in molecular weight, demonstrating that a set of varied molecular weight peptides was responsible for the antioxidant characteristics of black bean protein hydrolysates.

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“…Every year, the seafood processing industry discards a large amount of by-products, including viscera, shells, heads, squid pens, fins, and bones, even though they could be recycled to produce bioactive compounds like gelatin [1][2][3][4], enzymes [4][5][6][7][8][9][10][11][12][13][14][15][16][17], chitin [8,[18][19][20][21][22][23][24][25][26], chitin oligomers [7,11,12], α-glucosidase inhibitors (aGI) [27][28][29][30][31], carotenoids [32,33], and bioactive peptides [34][35][36][37][38][39][40]. Consequently, much research has gone into converting these by-products into bioactive products that have potential applications in biotechnological, agricultural, nutritional, pharmaceutical, and biomedical industries [1,…”

Section: Introductionmentioning

confidence: 99%

Conversion of Shrimp Head Waste for Production of a Thermotolerant, Detergent-Stable, Alkaline Protease by Paenibacillus sp.

et al. 2019

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Fishery processing by-products have been of great interest to researchers due to their beneficial applications in many fields. In this study, five types of marine by-products, including demineralized crab shell, demineralized shrimp shell, shrimp head, shrimp shell, and squid pen, provided sources of carbon and nitrogen nutrition by producing a protease from Paenibacillus sp. TKU047. Strain TKU047 demonstrated the highest protease productivity (2.98 U/mL) when cultured for two days on a medium containing 0.5% of shrimp head powder (SHP). The mass of TKU047 protease was determined to be 32 kDa (approximately). TKU047 protease displayed optimal activity at 70–80 °C and pH 9, with a pH range of stability from 6 to 11. TKU047 protease also showed stability in solutions containing surfactants and detergents. Based on its excellent properties, Paenibacillus sp. TKU047 protease may be a feasible candidate for inclusion in laundry detergents.

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Multiobjective optimization of the antioxidant activities of horse mackerel hydrolysates produced with protease mixtures

Cited by 19 publications

References 41 publications

Production of Fish Protein Hydrolysates from Scyliorhinus canicula Discards with Antihypertensive and Antioxidant Activities by Enzymatic Hydrolysis and Mathematical Optimization Using Response Surface Methodology

Production of Fish Protein Hydrolysates from Scyliorhinus canicula Discards with Antihypertensive and Antioxidant Activities by Enzymatic Hydrolysis and Mathematical Optimization Using Response Surface Methodology

Improving antioxidant activity of black bean protein by hydrolysis with protease combinations

Conversion of Shrimp Head Waste for Production of a Thermotolerant, Detergent-Stable, Alkaline Protease by Paenibacillus sp.

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