Multinuclear Yeast <i>Magnusiomyces (Dipodascus, Endomyces) magnusii</i> is a Promising Isobutanol Producer

Kurylenko, Olena; Ruchała, Justyna; Dmytruk, Kostyantyn V.; Abbas, Charles A.; Sibirny, Andriy А.

doi:10.1002/biot.201900490

Cited by 12 publications

(3 citation statements)

References 47 publications

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“…This approach to the accumulation of more than 0.7 g/L of isobutanol. All the data clearly show that E. magnusii is of great potential as a butanol and n-butanol producer [13].…”

Section: Introductionmentioning

confidence: 78%

The Lipid Profile of the Endomyces magnusii Yeast upon the Assimilation of the Substrates of Different Types and upon Calorie Restriction

Deryabina

Kokoreva

Klein

et al. 2022

JoF

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The study analyzes the dynamics in the lipid profile of the Endomyces magnusii yeast during the long-lasting cultivation using the substrates of “enzymatic” or “oxidative” type. Moreover, we studied its changes upon calorie restriction (CR) (0.5% glucose) and glucose depletion (0.2% glucose). Di-(DAGs), triacylglycerides (TAGs) and free fatty acids (FFAs) dominate in the storage lipid fractions. The TAG level was high in all the cultures tested and reached 80% of the total lipid amount. While being cultured on 2% substrates, the level of storage lipids decreased at the four-week stage, whereas upon CR their initially low amount doubled. Phosphatidylethanolamines (PE), sterols (St) (up to 62% of total lipids), phosphatidylcholines (PC), and phosphatidic acids (PA) (more than 40% of total lipids) were dominating in the membrane lipids of E magnusii. Upon CR at the late stationary growth stages (3–4 weeks), the total level of membrane lipid was two-fold higher than those on glycerol and 2% glucose. The palmitic acid C16:0 (from 10 to 23%), the palmitoleic acid C16:1 (from 4.3 to 15.9%), the oleic acid C18:1 (from 23.4 to 59.2%), and the linoleic acid C18:2 (from 10.8 to 49.2%) were the dominant fatty acids (FAs) of phospholipids. Upon glucose depletion (0.2% glucose), the total amount of storage and membrane lipids in the cells was comparable to that in the cells both on 2% and 0.5% glucose. High levels of PC and sphingolipids (SL) at the late stationary growth stages and an increased PA level throughout the whole experiment were typical for the membrane lipids composition upon the substrate depletion. There was shown a crucial role of St, PA, and a high share of the unsaturated FAs in the membrane phospholipids upon the adaptation of the E. magnusii yeast to the long-lasting cultivation upon the substrate restriction is shown. The autophagic processes in some fractions of the cell population provide the support of high level of lipid components at the late stages of cultivation upon substrate depletion under the CR conditions. CR is supposed to play the key role in regulating the lipid synthesis and risen resistance to oxidative stress, as well as its possible biotechnological application.

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“…This approach to the accumulation of more than 0.7 g/L of isobutanol. All the data clearly show that E. magnusii is of great potential as a butanol and n-butanol producer [13].…”

Section: Introductionmentioning

confidence: 78%

The Lipid Profile of the Endomyces magnusii Yeast upon the Assimilation of the Substrates of Different Types and upon Calorie Restriction

Deryabina

Kokoreva

Klein

et al. 2022

JoF

View full text Add to dashboard Cite

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“…They also developed advanced transformation protocol in which strong constitutive promoter TEF1 cloned and created the reporter system to test promoter strength. These methods also allowed to express heterologous S. cerevisiae ILV2 gene coding for acetolactate synthase which led to advanced isobutanol production of 0.7 g/ L [ 20 ].…”

Section: Current Isobutanol Production Strategies Using Microorganismsmentioning

confidence: 99%

Microbial engineering for the production of isobutanol: current status and future directions

et al. 2021

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Fermentation-derived alcohols have gained much attention as an alternate fuel due to its minimal effects on atmosphere. Besides its application as biofuel it is also used as raw material for coating resins, deicing fluid, additives in polishes, etc. Among the liquid alcohol type of fuels, isobutanol has more advantage than ethanol. Isobutanol production is reported in native yeast strains, but the production titer is very low which is about 200 mg/L. In order to improve the production, several genetic and metabolic engineering approaches have been carried out. Genetically engineered organism has been reported to produce maximum of 50 g/L of isobutanol which is far more than the native strain without any modification. In bacteria mostly last two steps in Ehrlich pathway, catalyzed by enzymes ketoisovalerate decarboxylase and alcohol dehydrogenase, are heterologously expressed to improve the production. Native Saccharomyces cerevisiae can produce isobutanol in negligible amount since it possesses the pathway for its production through valine degradation pathway. Further modifications in the existing pathways made the improvement in isobutanol production in many microbial strains. Fermentation using cost-effective lignocellulosic biomass and an efficient downstream process can yield isobutanol in environment friendly and sustainable manner. The present review describes the various genetic and metabolic engineering practices adopted to improve the isobutanol production in microbial strains and its downstream processing.

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“…In contrast, C. parapsilosis can cause infections, especially in people with a weak immune system. M. magnusii, which is a unique yeast created by forming large multinuclear cells, has been studied less than the other yeast species [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Effect of a non-thermal atmospheric pressure plasma jet on four different yeasts

Siadati

Petkova

Kenari

et al. 2020

J. Phys. D: Appl. Phys.

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Non-thermal atmospheric pressure plasmas are known to be an effective method for the inactivation of microorganisms. However, yeasts have proved to be more resistant to provided treatments. We investigated the influence of an Argon plasma jet on the inactivation of four different yeasts; Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida parapsilosis and Magnusiomyces magnusii. Results from direct plasma treatment on the yeasts in distilled water, as the most effective technique we found, are presented here. The surviving yeasts after 5, 7, and 10 min plasma exposures were evaluated visually and quantitatively. Quantitative results showed strong reductions in the survival rates after the plasma treatment. The longest plasma exposure time yielded the least survival. Moreover, how long the inactivations lasted was evaluated by assessing the treated samples after 1 h and again after 2 h. The survival rates were further decreased within these storage times. In the 10 min treated-2 h stored samples, the rates dropped to 5% or even below, depending on the yeast species. High concentrations of reactive oxygen and nitrogen species in the plasma-air-water interacting system were detected by optical emission spectroscopy of the plasma jet and also by measuring concentrations of H2O2, NO2 − and NO3 − in plasma activated water (as the surrounding liquid for the yeasts). The conductivity and pH of the treated water was measured as well. The level of the conductivity increased along with the decrease in pH. Synergistic effects of these chemical reactive species in acidified water resulted in the yeast inactivation.

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Multinuclear Yeast Magnusiomyces (Dipodascus, Endomyces) magnusii is a Promising Isobutanol Producer

Cited by 12 publications

References 47 publications

The Lipid Profile of the Endomyces magnusii Yeast upon the Assimilation of the Substrates of Different Types and upon Calorie Restriction

The Lipid Profile of the Endomyces magnusii Yeast upon the Assimilation of the Substrates of Different Types and upon Calorie Restriction

Microbial engineering for the production of isobutanol: current status and future directions

Effect of a non-thermal atmospheric pressure plasma jet on four different yeasts

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