Multimodality imaging to assess immediate response to irreversible electroporation in a rat liver tumor model

Zhang, Y.; White, Sarah B.; Nicolai, J.; West, Derek; Zhang, Z.; Kim, D.; Goodwin, Alan Lee; Miller, F.; Omary, Reed A.; Larson, Andrew C.

doi:10.1016/j.jvir.2013.01.294

Cited by 6 publications

(9 citation statements)

References 22 publications

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“…and B.W., 2 years of experience). Using a BTX Electroporator (ECM830; Harvard Apparatus, Holliston, MA), 8 pulses at 2 kV were delivered with a 100‐µs duration and 100‐ms spacing . After the IRE procedure, the abdominal incisions were closed with a 2‐layer technique.…”

Section: Methodsmentioning

confidence: 99%

Transcatheter intra‐arterial perfusion (TRIP)‐MRI biomarkers help detect immediate response to irreversible electroporation of rabbit VX2 liver tumor

Zhou

Pan

Sun

et al. 2019

Magnetic Resonance in Med

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Purpose Irreversible electroporation (IRE) is a nonthermal tissue ablation technique that represents a promising treatment option for unresectable liver tumors, but the effectively treated zone cannot be reliably predicted. We investigate the potential benefit of transcatheter intra‐arterial perfusion (TRIP) ‐MRI for the early noninvasive differentiation of IRE zone from surrounding reversibly electroporated (RE) zone. Methods Seventeen rabbits with VX2 liver tumors were scanned with morphological and contrast‐enhanced MRI sequences approximately 30 min after IRE tumor ablation. Quantitative TRIP‐MRI perfusion parameters were evaluated in IRE zone and RE zone, defined according to histology. MRI and histology results were compared among zones using Wilcoxon rank‐sum tests and correlations were evaluated by Pearson’s correlation coefficient. Results There were significant differences in area under the curve, time to peak, maximum and late enhancement, wash‐in and wash‐out rates in the tumor IRE zones compared with the boundary tumor RE zones and untreated tumors. Histology showed significantly fewer tumor cells, microvessels and significantly more apoptosis in tumor IRE zones compared with tumor RE zones (−51%, −66% and +185%, respectively) and untreated tumors (−60%, −67%, and +228%, respectively). A strong correlation was observed between MRI and histology measurements of IRE zones (r = 0.948) and RE zones (r = 0.951). Conclusion TRIP‐MRI demonstrated the potential to detect immediate perfusion changes following IRE liver tumor ablation and effectively differentiate the IRE zone from the surrounding tumor RE zone.

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Section: Methodsmentioning

confidence: 99%

Transcatheter intra‐arterial perfusion (TRIP)‐MRI biomarkers help detect immediate response to irreversible electroporation of rabbit VX2 liver tumor

Zhou

Pan

Sun

et al. 2019

Magnetic Resonance in Med

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“…Two paralleled MRI-compatible needle electrodes with 1-cm spacing (platinum-iridium materials; Microprobes, Gaithersburg, MD) were inserted into the center of the left median lobe with 1cm depth. Next, the electrodes were connected to the IRE generator (ECM830; Harvard Apparatus, Holliston, MA) for application of 2000-V square wave pulses (number of pulses, 10; each pulse duration, 100 μs; the interval between pulses, 100 ms) to the targeted tissue (7,15).…”

Section: Ire Proceduresmentioning

confidence: 99%

Transcatheter Intraarterial Perfusion MRI Approaches to Differentiate Reversibly Electroporated Penumbra From Irreversibly Electroporated Zones in Rabbit Liver

et al. 2020

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Rationale and Objectives: To investigate whether transcatheter intraarterial perfusion (TRIP) magnetic resonance imaging (MRI) can differentiate reversible electroporation (RE) zones from irreversible electroporation (IRE) zones immediately after IRE procedure in the rabbit liver. Materials and Methods:All studies were approved by the institutional animal care and use committee and performed in accordance with institutional guidelines. A total of thirteen healthy New Zealand White rabbits were used. After selective catheterization of the hepatic artery under X-ray fluoroscopy, we acquired TRIP-MRI at 20 minutes post-IRE using 3 mL of 5% intraarterial gadopentetate dimeglumine. Semi-quantitative (peak enhancement, PE; time to peak, TTP; wash-in slope, WIS; areas under the time-intensity curve, AUT, over 30, 60, 90, 120, 150, and 180 seconds after the initiation of enhancement) and quantitative (K trans , ve, and vp) TRIP-MRI parameters were calculated. The relationships between TRIP-MRI parameters 2 and histological measurements and the differential ability of TRIP-MRI parameters was assessed.

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“…Magnetic resonance (MR) imaging is generally considered a more accurate imaging technique for detecting liver tumors because of its superior soft-tissue contrast, particularly for smaller lesions. However, all of these clinical imaging techniques can depict only the morphologic changes occurring after IRE or show alterations in areas of perfusion if a contrast medium is supplied (3,17). Therefore, the therapeutic responses are currently identified only by changes in tumor size, which generally occur only weeks or months after therapy initiation (18).…”

Section: Tumor Cell Culture and Animal Modelmentioning

confidence: 99%

“…(1). Most recent studies have shown that IRE induces alterations to the cell membrane in both tumor and normal tissues (nanopores were observed on images from transmission electron microscopy) (2,3). IRE can ablate large volumes of tissue without inducing thermal effects; this ability offers advantages over existing thermal ablation methods (4)(5)(6).…”

Section: Author Contributionsmentioning

confidence: 99%

“…However, MR imaging can also provide additional qualitative and quantitative information about tissue function, microstructure, and perfusion (eg, through use of diffusion-weighted these methods have also demonstrated several critical drawbacks that can limit efficacy in the clinical setting (4-6). IRE involves targeted delivery of electrical pulses to induce cell death through permanent cell membrane permeabilization (2,3). One of the most relevant parameters that affects therapeutic efficacy is the amount of viable tumor tissue left after the procedure.…”

Section: Figurementioning

confidence: 99%

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¹⁸F-FDG PET Biomarkers Help Detect Early Metabolic Response to Irreversible Electroporation and Predict Therapeutic Outcomes in a Rat Liver Tumor Model

Wang¹,

Su²,

Lyu³

et al. 2018

Radiology

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Purpose To test the hypothesis that biomarkers of fluorine 18 (F) fluorodeoxyglucose (FDG) positron emission tomography (PET) can be used for the early detection of therapeutic response to irreversible electroporation (IRE) of liver tumor in a rodent liver tumor model. Materials and Methods The institutional animal care and use committee approved this study. Rats were inoculated with McA-RH7777 liver tumor cells in the left median and left lateral lobes. Tumors were allowed to grow for 7 days to reach a size typically at least 5 mm in longest diameter, as verified with magnetic resonance (MR) imaging. IRE electrodes were inserted, and eight 100-μsec, 2000-V pulses were applied to ablate the tumor tissue in the left median lobe. Tumor in the left lateral lobe served as a control in each animal. PET/computed tomography (CT) and MR imaging measurements were performed at baseline and 3 days after IRE for each animal. Additional MR imaging measurements were obtained 14 days after IRE. After 14-day follow-up MR imaging, rats were euthanized and tumors harvested for hematoxylin-eosin, CD34, and caspase-3 staining. Change in the maximum standardized uptake value (ΔSUV) was calculated 3 days after IRE. The maximum lesion diameter change (ΔD) was measured 14 days after IRE by using axial T2-weighted imaging. ΔSUV and ΔD were compared. The apoptosis index was calculated by using caspase-3-stained slices of apoptotic tumor cells. Pearson correlation coefficients were calculated to assess the relationship between ΔSUV at 3 days and ΔD (or apoptosis index) at 14 days after IRE treatment. Results ΔSUV ΔD, and apoptosis index significantly differed between treated and untreated tumors (P < .001 for all). In treated tumors, there was a strong correlation between ΔSUV 3 days after IRE and ΔD 14 days after IRE (R = 0.66, P = .01) and between ΔSUV 3 days after IRE and apoptosis index 14 days after IRE (R = 0.57, P = .04). Conclusion F-FDG PET imaging biomarkers can be used for the early detection of therapeutic response to IRE treatment of liver tumors in a rodent model. RSNA, 2017.

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Multimodality imaging to assess immediate response to irreversible electroporation in a rat liver tumor model

Cited by 6 publications

References 22 publications

Transcatheter intra‐arterial perfusion (TRIP)‐MRI biomarkers help detect immediate response to irreversible electroporation of rabbit VX2 liver tumor

Transcatheter intra‐arterial perfusion (TRIP)‐MRI biomarkers help detect immediate response to irreversible electroporation of rabbit VX2 liver tumor

Transcatheter Intraarterial Perfusion MRI Approaches to Differentiate Reversibly Electroporated Penumbra From Irreversibly Electroporated Zones in Rabbit Liver

¹⁸F-FDG PET Biomarkers Help Detect Early Metabolic Response to Irreversible Electroporation and Predict Therapeutic Outcomes in a Rat Liver Tumor Model

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Multimodality imaging to assess immediate response to irreversible electroporation in a rat liver tumor model

Cited by 6 publications

References 22 publications

Transcatheter intra‐arterial perfusion (TRIP)‐MRI biomarkers help detect immediate response to irreversible electroporation of rabbit VX2 liver tumor

Transcatheter intra‐arterial perfusion (TRIP)‐MRI biomarkers help detect immediate response to irreversible electroporation of rabbit VX2 liver tumor

Transcatheter Intraarterial Perfusion MRI Approaches to Differentiate Reversibly Electroporated Penumbra From Irreversibly Electroporated Zones in Rabbit Liver

18F-FDG PET Biomarkers Help Detect Early Metabolic Response to Irreversible Electroporation and Predict Therapeutic Outcomes in a Rat Liver Tumor Model

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¹⁸F-FDG PET Biomarkers Help Detect Early Metabolic Response to Irreversible Electroporation and Predict Therapeutic Outcomes in a Rat Liver Tumor Model