Multimodal Magnetic Core–Shell Nanoparticles for Effective Stem‐Cell Differentiation and Imaging

Shah, Birju P.; Yin, Perry T.; Ghoshal, Shraboni; Lee, Ki‐Bum

doi:10.1002/ange.201302245

Cited by 15 publications

(5 citation statements)

References 44 publications

(25 reference statements)

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“… 37 Then, citrate-capped MCNPs were synthesized according to previously reported methods with slight modifications. 32 The as-synthesized citrate-capped MCNPs were found to have a hydrodynamic diameter of 15.4 nm ( Figure 2 b). Thereafter these MCNPs were coated with branched polyethylenimine (PEI, MW = 10 kDa) via electrostatic interactions to afford an overall positive charge to the complex.…”

Section: Resultsmentioning

confidence: 98%

“… 29 − 31 Additionally, our group as well as others have recently demonstrated that these MNPs can be further modified with a gold shell, thus having both magnetic and plasmonic properties that can be utilized for various biological applications including cell targeting, imaging, and chemotherapy. 32 …”

mentioning

confidence: 99%

“…Second, the magnetic core allowed for magnetically facilitated delivery of the MCNP-ATAP constructs, thus leading to enhanced cellular uptake. 32 , 34 , 35 In addition, the magnetic core of the MCNPs enabled induction of localized hyperthermia, 36 which could act in a complementary fashion with the ATAP moieties to synergistically enhance cancer cell apoptosis. Thus, we hypothesized that our MCNPs would serve as a versatile platform enabling the application of two orthogonal yet complementary modalities, thereby resulting in significant improvements in cancer therapy.…”

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confidence: 99%

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Core–Shell Nanoparticle-Based Peptide Therapeutics and Combined Hyperthermia for Enhanced Cancer Cell Apoptosis

et al. 2014

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Mitochondria-targeting peptides have garnered immense interest as potential chemotherapeutics in recent years. However, there is a clear need to develop strategies to overcome the critical limitations of peptides, such as poor solubility and the lack of target specificity, which impede their clinical applications. To this end, we report magnetic core–shell nanoparticle (MCNP)-mediated delivery of a mitochondria-targeting pro-apoptotic amphipathic tail-anchoring peptide (ATAP) to malignant brain and metastatic breast cancer cells. Conjugation of ATAP to the MCNPs significantly enhanced the chemotherapeutic efficacy of ATAP, while the presence of targeting ligands afforded selective delivery to cancer cells. Induction of MCNP-mediated hyperthermia further potentiated the efficacy of ATAP. In summary, a combination of MCNP-mediated ATAP delivery and subsequent hyperthermia resulted in an enhanced effect on mitochondrial dysfunction, thus resulting in increased cancer cell apoptosis.

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Section: Resultsmentioning

confidence: 98%

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confidence: 99%

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confidence: 99%

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Core–Shell Nanoparticle-Based Peptide Therapeutics and Combined Hyperthermia for Enhanced Cancer Cell Apoptosis

et al. 2014

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“… 45 − 47 Third, the AuNP backbone of NanoScript can be replaced with other types of nanoparticles, such as upconverting nanoparticles and magnetic core–shell nanoparticles, for potential applications such as tracking gene regulation in real time through infrared imaging or magnetic resonance imaging (MRI), respectively. 43 , 48 Finally, the most advantageous feature of NanoScript is its tunable components, by simply redesigning the hairpin polyamide DBD sequence to target regulatory genes such as those involved in differentiation. However, it should be noted that, due to the short DBD targeting sequence, there is a possibility for interactions with off-target genes; hence, further optimization by designing DBDs with longer sequences to target longer DNA sequences would further enhance the NanoScript platform.…”

Section: Discussionmentioning

confidence: 99%

NanoScript: A Nanoparticle-Based Artificial Transcription Factor for Effective Gene Regulation

et al. 2014

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Transcription factor (TF) proteins are master regulators of transcriptional activity and gene expression. TF-based gene regulation is a promising approach for many biological applications; however, several limitations hinder the full potential of TFs. Herein, we developed an artificial, nanoparticle-based transcription factor, termed NanoScript, which is designed to mimic the structure and function of TFs. NanoScript was constructed by tethering functional peptides and small molecules called synthetic transcription factors, which mimic the individual TF domains, onto gold nanoparticles. We demonstrate that NanoScript localizes within the nucleus and initiates transcription of a reporter plasmid by over 15-fold. Moreover, NanoScript can effectively transcribe targeted genes on endogenous DNA in a nonviral manner. Because NanoScript is a functional replica of TF proteins and a tunable gene-regulating platform, it has great potential for various stem cell applications.

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“…Recently, Lee and coworkers used a magnetofection-based approach to efficiently delivery MNPs into neural stem cells (NSCs) to induce neuronal differentiation. 38 They used zinc-doped MNPs, ZnFe 2 O 4 , as a core and synthesized a gold shell to develop a magnetic core-shell nanoparticle (MCNP) (Figure 3(a)-(c)). The purpose of the outer gold shell is to increase biocompatibility, enable multiple biomolecules to anchor onto a single nanoparticle, and prevent free radical formation from the MNP core.…”

Section: Magnetically Facilitated Delivery For Enhanced Stem Cell Difmentioning

confidence: 99%

Probing stem cell behavior using nanoparticle‐based approaches

Patel

Lee

2015

WIREs Nanomed Nanobiotechnol

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Stem cells hold significant clinical potential to treat numerous debilitating diseases and injures that currently have no treatment plan. While several advances have been made in developing stem cell platforms and methods to induce their differentiation, there are two critical aspects need to be addressed: (1) efficient delivery of nucleic acids and small molecules for stem cell differentiation, and (2) effective, noninvasive, and real-time tracking of transplanted stem cells. To address this, there has been a trend of utilizing various types of nanoparticles to not only deliver biomolecules to targeted site but also track the location of transplanted stem cells in real time. Over the past decade, various types of nanoparticles, including magnetic nanoparticles, silica nanoparticles, quantum dots, and gold nanoparticles, have been developed to serve as vehicles for targeted biomolecule delivery. In addition of being biocompatible without causing adverse side effect to stem cells, these nanoparticles have unique chemical and physical properties that allow tracking and imaging in real time using different imaging instruments that are commonly found in hospitals. A summary of the landmark and progressive demonstrations that utilize nanoparticles for stem cell application is described.

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Multimodal Magnetic Core–Shell Nanoparticles for Effective Stem‐Cell Differentiation and Imaging

Cited by 15 publications

References 44 publications

Core–Shell Nanoparticle-Based Peptide Therapeutics and Combined Hyperthermia for Enhanced Cancer Cell Apoptosis

Core–Shell Nanoparticle-Based Peptide Therapeutics and Combined Hyperthermia for Enhanced Cancer Cell Apoptosis

NanoScript: A Nanoparticle-Based Artificial Transcription Factor for Effective Gene Regulation

Probing stem cell behavior using nanoparticle‐based approaches

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