Multimodal imaging using optical coherence tomography and endolaryngeal ultrasonography in a new rabbit VX2 laryngeal cancer model

Abstract

This study is the first experimental trial, assessing the value of multimodal imaging using OCT and EUS in a rabbit VX2 laryngeal tumor model. Combining OCT and EUS helped to identify changes in laryngeal mucous membranes, and could potentially be used to identify laryngeal tumors and predict how tumors progress. This combined modality could help in determining tumor extent, assisting in diagnosis, and establishing a treatment plan for laryngeal cancer.

Spatiotemporal Optoacoustic Mapping of Tumor Hemodynamics in a Clinically Relevant Orthotopic Rabbit Model of Head and Neck Cancer

“…The common sites of tumor implantation in this model include the leg muscle, liver, and auricles [15] , [16] , [17] . Studies have also established VX2 tumors in the buccal mucosa, larynx, and esophagus [18] , [19] , [20] . To mimic thick neck masses seen in humans, we developed an orthotopic neck tumor model by surgically implanting tumor tissue into the sternohyoid muscle of rabbits.…”

Development and Validation of a Clinically Relevant Workflow for MR-Guided Volumetric Arc Therapy in a Rabbit Model of Head and Neck Cancer

“…While mouse models are widely utilized in research, the clinical or translational value of radiation studies in murine systems remains unclear. As a result, there has been increased interest in developing and applying canine (dogs), feline (cats), and leporine (rabbits) models of cancer for preclinical research [21][22][23][24][25][26][27]. However, the literature on the implementation of IG-VMAT in large animal models of cancer, especially HNSCC, is limited.…”

“…Similarly, in the head and neck region, differences in salivary gland anatomy between rodents and humans exist that should be considered in the context of RT treatment planning and toxicity assessment [20]. Given these limitations of small animal models, there has been increased interest in the utilization of large animal models of HNSCC for preclinical studies [21][22][23]. In this regard, we have recently reported on a novel orthotopic rabbit model of HNSCC based on surgical transplantation of Shope cottontail rabbit papilloma virus associated VX2 tumors into the neck of rabbits [24].…”

Biocompatible astaxanthin as a novel marine-oriented agent for dual chemo-photothermal therapy

“…VX2 cells, which are epithelial cells originating from cancer, were selected to create tumors on the eyes of rabbits. Due to its relatively fast growth, this cell line is often used for small-medium animal models to evaluate preclinical cancer diagnosis and treatment [40]. These cell lines were used and cultured on monolayers in DMEM and DMEM-F12 (Thermo Scientific, Waltham, MA, USA), which were supplemented with 10% fetal bovine serum (FBS, Thermo Scientific, Waltham, MA, USA).…”