Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source

Segawa, Hiroki; Okuno, Masanari; Leproux, Philippe; Couderc, Vincent; Ozawa, Toshio; Kano, Hideaki

doi:10.2116/analsci.31.299

Cited by 14 publications

(7 citation statements)

References 37 publications

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“…As a consequence, shorter pulses create higher peak powers. However, also longer pulses can be used [3][4][5][6][7][8][9]. Particularly, at same peak powers the repetition rate can be lowered, thus giving the same duty cycle and, hence, the same average power.…”

Section: Introductionmentioning

confidence: 99%

Two-photon microscopy using fiber-based nanosecond excitation

Karpf

Eibl

Sauer

et al. 2016

Biomed. Opt. Express

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Two-photon excitation fluorescence (TPEF) microscopy is a powerful technique for sensitive tissue imaging at depths of up to 1000 micrometers. However, due to the shallow penetration, for in vivo imaging of internal organs in patients beam delivery by an endoscope is crucial. Until today, this is hindered by linear and non-linear pulse broadening of the femtosecond pulses in the optical fibers of the endoscopes. Here we present an endoscope-ready, fiber-based TPEF microscope, using nanosecond pulses at low repetition rates instead of femtosecond pulses. These nanosecond pulses lack most of the problems connected with femtosecond pulses but are equally suited for TPEF imaging. We derive and demonstrate that at given cw-power the TPEF signal only depends on the duty cycle of the laser source. Due to the higher pulse energy at the same peak power we can also demonstrate single shot two-photon fluorescence lifetime measurements. Leproux, "Multicolor multiphoton microscopy based on a nanosecond supercontinuum laser source," J. Biophoton. In press (2016). 10. G. Donnert, C. Eggeling, and S. W. Hell, "Major signal increase in fluorescence microscopy through dark-state relaxation," Nat. Methods 4(1), 81-86 (2007). 11. M. Goeppert-Mayer, "Über Elementarakte mit zwei Quantensprüngen," Ann. Phys. 9(3), 273-294 (1931). ©2016 Optical Society of America #261064Received 11 12. S. Karpf, M. Eibl, W. Wieser, T. Klein, and R. Huber, "A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy," Nat. Commun. 6, 6784 (2015). 13. S. Tang, J. Liu, T. B. Krasieva, Z. Chen, and B. J. Tromberg, "Developing compact multiphoton systems using femtosecond fiber lasers," J. Biomed. Opt. 14, 030508 (2009). 14. F. Knorr, D. R. Yankelevich, J. Liu, S. Wachsmann-Hogiu, and L. Marcu, "Two-photon excited fluorescence lifetime measurements through a double-clad photonic crystal fiber for tissue micro-endoscopy," J. Biophotonics 5(1), 14-19 (2012). 15. K. Taira, T. Hashimoto, and H. Yokoyama, "Two-photon fluorescence imaging with a pulse source based on a 980-nm gain-switched laser diode," Opt. Express 15(5), 2454-2458 (2007). 16. R. H. Stolen and C. Lin, "Self-phase-modulation in silica optical fibers," Phys. Rev. A 17(4), 1448-1453 (1978). 17. E. P. Ippen and R. H. Stolen, "Stimulated Brillouin scattering in optical fibers," Appl. Phys. Lett. 21(11), 539-541 (1972 478-492 (2010). 21. N. S. Makarov, M. Drobizhev, and A. Rebane, "Two-photon absorption standards in the 550-1600 nm excitation wavelength range," Opt.

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Section: Introductionmentioning

confidence: 99%

Two-photon microscopy using fiber-based nanosecond excitation

Karpf

Eibl

Sauer

et al. 2016

Biomed. Opt. Express

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“…Эти источники имеют ряд преимуществ: высокую частоту повторения, низкую энергию импульсов, большую спектральную перестраиваемость и высокую среднюю мощность. Другие монохроматические источники также использовались незначительно, такие как наносекундные [8], пикосекундные [4] и субпикосекундные лазеры [9]. Различные длительности импульсов от 100 фс до 100 пс с постоянной частотой повторения позволяют получать аналогичные многофотонные взаимодействия [10][11][12] при условии достаточной средней мощности и соблюдения уравнения (1).…”

Section: вступлениеunclassified

Многоцветная Многофотонная Микроскопия На Основе Наносекундного Источника Суперконтинуума

Лефорт¹,

О'Конор²,

Бланке³

et al. 2020

PhotonicsRussia

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Оптические измерения *Примечание редактора: статья из журнала дана в переводе с согласия авторов и в полном согласии с условиями открытого доступа на условиях лицензии Creative Commons Attribution-Non Commercial-No Derivs, которая разрешает использование и распространение на любом носителе при условии, что оригинальная работа должным образом цитируется.Multicolor multiphoton microscopy based on a nanosecond supercontinuum laser source.

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“…Modern optical-spectroscopic methods could be ideal candidates. Advanced laser techniques, such as Raman spectroscopy and its modifications 6–8 , fluorescence spectroscopy and microscopy 7,9 , fluorescence lifetime imaging microscopy (FLIM) 10,11 , fluorescence resonance energy transfer (FRET) 11 , harmonic generation microscopy 12 , and Fourier Transform Infrared Spectroscopy (FTIR) 13 allow the study of organelles organization, structural and molecular biochemical processes inside living cells without disturbing the living processes.…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopy on live mouse early embryo while it continues to develop into blastocyst in vitro

Perevedentseva

Krivokharchenko

Karmenyan

et al. 2019

Sci Rep

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Laser based spectroscopic methods can be versatile tools in investigating early stage mammalian embryo structure and biochemical processes in live oocytes and embryos. The limiting factor for using the laser methods in embryological studies is the effect of laser irradiation on the ova. The aim of this work is to explore the optimal parameters of the laser exposure in Raman spectroscopic measurements applicable for studying live early embryos in vitro without impacting their developmental capability. Raman spectra from different areas of mouse oocytes and 2-cells embryos were measured and analyzed. The laser power and exposure time were varied and further embryo development was evaluated to select optimal conditions of the measurements. This work demonstrates safe laser irradiation parameters can be selected, which allow acquisition of Raman spectra suitable for further analysis without affecting the early mouse embryo development in vitro up to morphologically normal blastocyst. The estimation of living embryo state is demonstrated via analysis and comparison of the spectra from fertilized embryo with the spectra from unfertilized oocytes or embryos subjected to UV laser irradiation. These results demonstrate the possibility of investigating preimplantation mammalian embryo development and estimating its state/quality. It will have potential in developing prognosis of mammalian embryos in assisted reproductive technologies.

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Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source

Cited by 14 publications

References 37 publications

Two-photon microscopy using fiber-based nanosecond excitation

Two-photon microscopy using fiber-based nanosecond excitation

Многоцветная Многофотонная Микроскопия На Основе Наносекундного Источника Суперконтинуума

Raman spectroscopy on live mouse early embryo while it continues to develop into blastocyst in vitro

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