Multimodal imaging approach to monitor browning of adipose tissue in vivo

Chan, Xin Hui Derryn; Balasundaram, Ghayathri; Attia, Amalina Binte Ebrahim; Goggi, Julian; Ramasamy, Boominathan; Han, Weiping; Olivo, Malini; Sugii, Shigeki

doi:10.1194/jlr.d083410

Cited by 12 publications

(12 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The discovery that white adipocytes can undergo a browning process to become metabolically active beige cells has attracted significant interest in the fight against obesity [ 33 ]. Hence, differently from white adipocytes, which are responsible for energy storage, brown adipocytes are key regulators in the dissipation of chemical energy as heat.…”

Section: Discussionmentioning

confidence: 99%

Effects of Genistein on Differentiation and Viability of Human Visceral Adipocytes

et al. 2018

View full text Add to dashboard Cite

Obesity can lead to pathological growth of adipocytes by inducing inflammation and oxidative stress. Genistein could be a potential candidate for the treatment of obesity due to its antioxidant properties. Specific kits were used to examine the effects of genistein vs adiponectin on human visceral pre-adipocytes differentiation, cell viability, mitochondrial membrane potential, and oxidative stress in pre-adipocytes and in white/brown adipocytes. Western Blot was performed to examine changes in protein activation/expression. Genistein increased human visceral pre-adipocytes differentiation and browning, and caused a dose-related improvement of cell viability and mitochondrial membrane potential. Similar effects were observed in brown adipocytes and in white adipocytes, although in white cells the increase of cell viability was inversely related to the dose. Moreover, genistein potentiated AMP-activated protein kinase (AMPK)/mitofusin2 activation/expression in pre-adipocytes and white/brown adipocytes and protected them from the effects of hydrogen peroxide. The effects caused by genistein were similar to those of adiponectin. The results obtained showed that genistein increases human visceral pre-adipocytes differentiation and browning, protected against oxidative stress in pre-adipocytes and white/brown adipocytes through mechanisms related to AMPK-signalling and the keeping of mitochondrial function.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Genistein on Differentiation and Viability of Human Visceral Adipocytes

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Visualization of can cer cells immediately after injection into the mouse heart was carried out using the GNR signal; the fluorescence of iRFP720 was recorded at later stages (up to 40 days after injection) [4]. The MSOT method allowed the real time monitoring of the white adipose tissue transformation into beige adipocytes during adrenergic stimulation, as well as of associated changes of lipid metabolism in mice [119].…”

Section: Nir Fluorescent Proteins In Advanced Visualization Technologiesmentioning

confidence: 99%

Near-Infrared Fluorescent Proteins and Their Applications

Karasev

Stepanenko

Rumyantsev

et al. 2019

Biochemistry Moscow

View full text Add to dashboard Cite

High transparency, low light scattering, and low autofluorescence of mammalian tissues in the near infrared (NIR) spectral range (~650 900 nm) open a possibility for in vivo imaging of biological processes at the micro and macroscales to address basic and applied problems in biology and biomedicine. Recently, probes that absorb and fluoresce in the NIR optical range have been engineered using bacterial phytochromes-natural NIR light absorbing photoreceptors that regulate metabolism in bacteria. Since the chromophore in all these proteins is biliverdin, a natural product of heme catabolism in mammalian cells, they can be used as genetically encoded fluorescent probes, similarly to GFP like fluores cent proteins. In this review, we discuss photophysical and biochemical properties of NIR fluorescent proteins, reporters, and biosensors and analyze their characteristics required for expression of these molecules in mammalian cells. Structural features and molecular engineering of NIR fluorescent probes are discussed. Applications of NIR fluorescent proteins and biosensors for studies of molecular processes in cells, as well as for tissue and organ visualization in whole body imaging in vivo, are described. We specifically focus on the use of NIR fluorescent probes in advanced imaging technologies that com bine fluorescence and bioluminescence methods with photoacoustic tomography.

show abstract

“…Positron emission tomography (PET) is a uniquely sensitive imaging method and numerous tracers have been assessed for their ability to quantify BAT. [ 18 F]FDG imaging is the current gold standard for imaging BAT and has been used to image beige fat, but detection is variable and dependent on stimulation through cold exposure or chemical stimulation [8], [9]. Other PET radiopharmaceuticals have been developed to quantify BAT mass including the cannabinoid receptor 1 imaging agent [ 18 F]FMPEP, the noradrenaline transporter agent [ 11 C]MRB, and the noradrenaline analogue [ 11 C]MHED; however, they have not been assessed for the ability to measure beige fat in vivo under unstimulated or thermoneutral conditions [10], [11], [12], [13].…”

Section: Introductionmentioning

confidence: 99%

Imaging adipose tissue browning using the TSPO-18kDa tracer [18F]FEPPA

Hartimath

Khanapur

Boominathan

et al. 2019

Molecular Metabolism

View full text Add to dashboard Cite

Objectives The browning of white adipose tissue (WAT) into beige has been proposed as a strategy to enhance energy expenditure to combat the growing epidemic of obesity. Research into browning strategies are hampered by the lack of sensitive, translatable, imaging tools capable of detecting beige fat mass non-invasively. [ 18 F]FDG is able to detect activated beige fat but provides little information on unstimulated beige fat mass. We have assessed the use of [ 18 F]FEPPA, a tracer for the TSPO-18KDa found on the outer mitochondrial membrane, as an alternative imaging agent capable of detecting unstimulated brown fat (BAT) and beige fat. Methods Female Balb/c mice (n = 5) were treated for 7 days with the β3 adrenergic agonist CL-316,243 to induce the browning of inguinal WAT (beige fat). Animals were imaged longitudinally with [ 18 F]FDG and [ 18 F]FEPPA and uptake in interscapular BAT and inguinal WAT assessed. The browning of inguinal WAT was confirmed using H&E and immunohistochemical detection of UCP-1 and TSPO. Results Repeated dosing with β3-adrenergic agonist CL-316,243 caused a significant increase in [ 18 F]FDG uptake in both interscapular BAT and inguinal WAT associated with the increased metabolic activity of brown and beige adipocytes respectively. [ 18 F]FEPPA uptake was likewise increased in inguinal WAT but showed no increase in BAT uptake due to stimulation over the same time course. Furthermore, inguinal WAT uptake was unaffected by pharmacological blockade, indicating that [ 18 F]FEPPA uptake is associated with the expression of mitochondria in BAT and beige adipocytes and independent of activation. Conclusion These data show that [ 18 F]FEPPA can detect BAT and newly formed beige fat under non-stimulated, thermoneutral conditions and that uptake after stimulation is linked to mitochondrial expression as opposed to activation.

show abstract

Multimodal imaging approach to monitor browning of adipose tissue in vivo

Cited by 12 publications

References 38 publications

Effects of Genistein on Differentiation and Viability of Human Visceral Adipocytes

Effects of Genistein on Differentiation and Viability of Human Visceral Adipocytes

Near-Infrared Fluorescent Proteins and Their Applications

Imaging adipose tissue browning using the TSPO-18kDa tracer [18F]FEPPA

Contact Info

Product

Resources

About