Multimodal evaluation of ultra-short laser pulses treatment for skin burn injuries

Santos, Moisés Oliveira dos; Latrive, Anne; Castro, P.; Rossi, Wagner de; Zorn, Telma Maria Tenório; Samad, Ricardo Elgul; Freitas, Anderson Zanardi de; César, Carlos L.; Vieira, Nilson D.; Zezéll, Denise Maria

doi:10.1364/boe.8.001575

Cited by 14 publications

(9 citation statements)

References 39 publications

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“…Infrared spectroscopy and several other optical methods have been proposed for the characterization of physical and chemical properties of skin. 13–19 Fourier transform infrared (FT-IR) spectroscopy provides information about the overall biochemical status of the examined sample based on the vibrations of molecular structural units (lipids, proteins, nucleic acids, and carbohydrates) that are infrared active. 20 The potential applications of FT-IR spectroscopy as a tool to study biological samples has been well demonstrated over the past years.…”

Section: Introductionmentioning

confidence: 99%

Monitoring the Progress and Healing Status of Burn Wounds Using Infrared Spectroscopy

et al. 2020

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Burns are one of the leading causes of morbidity worldwide and the most costly traumatic injuries. A better understanding of the molecular mechanisms in wound healing is required to accelerate tissue recovery and reduce the health economic impact. However, the standard techniques used to evaluate the biological events associated to wound repair are laborious, time-consuming, and/or require multiple assays/staining. Therefore, this study aims to evaluate the feasibility of Fourier transform infrared (FT-IR) spectroscopy to monitor the progress and healing status of burn wounds. Burn injuries were induced on Wistar rats by water vapor exposure and biopsied for further histopathological and spectroscopic evaluation at four time-points (3, 7, 14, and 21 days). Spectral data were preprocessed and compared by principal component analysis. Pairwise comparison of post-burn groups to each other revealed that metabolic activity induced by thermal injury decreases as the healing progresses. Higher amounts of carbohydrates, proteins, lipids, and nucleic acids were evidenced on days 3 and 7 compared to healthy skin and reduced amounts of these molecular structural units on days 14 and 21 post-burn. FT-IR spectroscopy was used to determine the healing status of a wound based on the biochemical information retained by spectral signatures in each phase of healing. Our findings demonstrate that FT-IR spectroscopy can monitor the biological events triggered by burn trauma as well as to detect the wound status including full recovery based on the spectral changes associated to the biochemical events in each phase.

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Section: Introductionmentioning

confidence: 99%

Monitoring the Progress and Healing Status of Burn Wounds Using Infrared Spectroscopy

et al. 2020

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“…At the beginning of laser exposure, laser beam irradiation results in the formation of blisters that later collapse. In previous studies, it was demonstrated that the tissue temperature was proportional to the exposure time and that the scattering coefficient increased with temperature [36], [37]. As shown in Fig.…”

Section: Discussionmentioning

confidence: 77%

Optical Coherence Tomography/Angiography-Guided Tumor Ablation With a Continuous-Wave Laser Diode

et al. 2020

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Laser ablation can be an effective modality for treatment, but it is complicated to apply continuous-wave (CW) light sources for laser ablation because of the unpredictable photothermal damage. In this study, an integrated theranostic system combining a low-cost CW laser diode with optical coherence tomography (OCT)/angiography (OCTA) was utilized for the in vivo ablation of tumor tissues. To examine the effect of laser exposure on tissue scattering characteristics, the OCT backscattering intensities of nonablated and ablated tissues were analyzed, and the effect on the skin microvasculature produced by laser ablation was quantitatively evaluated. Moreover, the integrated system and the proposed method were implemented for the treatment of skin tumor on the mouse model. The obtained results indicate that the developed laser ablation system can effectively remove tumor tissues with controllable photodamage under OCT/OCTA guidance and that the system cost may be significantly reduced by using the CW laser diode.

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“…V 1 , V 2 , V 3 , V 4 and V 5 are main blood vessels at the position of the white dotted line in Figure 3D,E. The blood flow of burn area reduced is due to thermal effects lead to inactivation of blood vessels, slowing or stopping of blood flow [40]. It is noticed that there is an increase in vascular area density and blood flow around the burned tissue, which is due to reserve capillaries that existed before the injury, and it has been recruited after the injury (known as capillary recruitment phenomena) [3].…”

Section: Resultsmentioning

confidence: 99%

“…4 and V 5 are main blood vessels at the position of the white dotted line inFigure 3D,E. The blood flow of burn area reduced is due to thermal effects lead to inactivation of blood vessels, slowing or stopping of blood flow[40].…”

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confidence: 99%

Towards quantitative assessment of burn based on photoacoustic and optical coherence tomography

Liu

Chen

Zhou

et al. 2020

Journal of Biophotonics

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Accurate and timely assessment of the severity of burn is essential for the treatment of burns. Currently, although most first-degree and thirddegree burns are easily diagnosed through visual inspection or auxiliary diagnostic methods, the seconddegree burn is still difficult to distinguish due to the ambiguity boundaries of second-degree with first-degree and third-degree burns. In this study, we proposed a non-invasive technique by combing photoacoustic imaging (PAI) and optical coherence tomography (OCT) to multi-parameter quantitatively assess the burns. The feasibility and capacity of the dualmode PAT/OCT for assessing the burns was first testified by tissuemimicking phantom and burn wounds in mouse pinna in vivo. The further

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Multimodal evaluation of ultra-short laser pulses treatment for skin burn injuries

Cited by 14 publications

References 39 publications

Monitoring the Progress and Healing Status of Burn Wounds Using Infrared Spectroscopy

Monitoring the Progress and Healing Status of Burn Wounds Using Infrared Spectroscopy

Optical Coherence Tomography/Angiography-Guided Tumor Ablation With a Continuous-Wave Laser Diode

Towards quantitative assessment of burn based on photoacoustic and optical coherence tomography

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