Multimodal biophotonic workstation for live cell analysis

Esseling, Michael; Kemper, Björn; Antkowiak, Maciej; Stevenson, David; Chaudet, Lionel; Neil, Mark A. A.; French, Paul M. W.; Bally, Gert von; Dholakia, Kishan; Denz, Cornélia

doi:10.1002/jbio.201100052

Cited by 19 publications

(9 citation statements)

References 23 publications

(23 reference statements)

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“…The force feedback can reduce the time of operations and increase the feasible complexity of tasks. The new capabilities offered by haptic optical tweezers will inspire study and diagnosis of new types of cells 105,106 or allow complex assembly of biomedical materials. 107 …”

Section: Discussionmentioning

confidence: 99%

Invited Article: A review of haptic optical tweezers for an interactive microworld exploration

Pacoret

Régnier²

2013

Review of Scientific Instruments

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This paper is the first review of haptic optical tweezers, a new technique which associates force feedback teleoperation with optical tweezers. This technique allows users to explore the microworld by sensing and exerting picoNewton-scale forces with trapped microspheres. Haptic optical tweezers also allow improved dexterity of micromanipulation and micro-assembly. One of the challenges of this technique is to sense and magnify picoNewton-scale forces by a factor of 10(12) to enable human operators to perceive interactions that they have never experienced before, such as adhesion phenomena, extremely low inertia, and high frequency dynamics of extremely small objects. The design of optical tweezers for high quality haptic feedback is challenging, given the requirements for very high sensitivity and dynamic stability. The concept, design process, and specification of optical tweezers reviewed here are focused on those intended for haptic teleoperation. In this paper, two new specific designs as well as the current state-of-the-art are presented. Moreover, the remaining important issues are identified for further developments. The initial results obtained are promising and demonstrate that optical tweezers have a significant potential for haptic exploration of the microworld. Haptic optical tweezers will become an invaluable tool for force feedback micromanipulation of biological samples and nano- and micro-assembly parts.

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Section: Discussionmentioning

confidence: 99%

Invited Article: A review of haptic optical tweezers for an interactive microworld exploration

Pacoret

Régnier²

2013

Review of Scientific Instruments

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“…Furthermore, using advanced pressure management systems, selected cells of interest (e.g., the cancer cells) can selectively be released after the analysis and subjected to further analysis, such as molecular biological assays (PCR) or activity testing with chemotherapeutic drugs. This approach is also compatible with recently described multimodal biophotonics workstation for live cell analysis [28].…”

Section: Discussionmentioning

confidence: 78%

Raman-Spectroscopy Based Cell Identification on a Microhole Array Chip

et al. 2014

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Circulating tumor cells (CTCs) from blood of cancer patients are valuable prognostic markers and enable monitoring responses to therapy. The extremely low number of CTCs makes their isolation and characterization a major technological challenge. For label-free cell identification a novel combination of Raman spectroscopy with a microhole array platform is described that is expected to support high-throughput and multiplex analyses. Raman spectra were registered from regularly arranged cells on the chip with low background noise from the silicon nitride chip membrane. A classification model was trained to distinguish leukocytes from myeloblasts (OCI-AML3) and breast cancer cells . The model was validated by Raman spectra of a mixed cell population. The high spectral quality, low destructivity and high classification accuracy suggests that this approach is promising for Raman activated cell sorting. OPEN ACCESSMicromachines 2014, 5 205

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“…Future prospects include the use of DHM for multimodal imaging [87], 3D cell tracking in a 3D environment, further analysis of the quantitative phase images for light scattering (see Ref. DHM in off-axis and self-interference configuration enables a vibration insensitive (hologram capture time in or below the millisecond range) and noncontact full-field measurement of dynamic cellular processes.…”

Section: Discussionmentioning

confidence: 99%