“…Candidate substrate materials must exhibit biocompatibility, while supporting cell proliferation, which is hypothesized to be a direct function of its stiffness. Bioengineered scaffold stiffness must be closely resemble that of native ECM and flexible to allow the contraction of growing cells . In the case of PCL membranes, 3T3 fibroblast cell proliferation was most significant on low stiffness samples .…”
Polycaprolactone (PCL), a synthetic biocompatible and biodegradable polymer generally used as a scaffold material for tissue engineering applications. The high stiffness and hydrophobicity of the PCL fiber mesh does not provide significant cell attachment and proliferation in cardiac tissue engineering. Towards this goal, the study focused on a compound of PCL and oligomer hydrogel [Bisphenol A ethoxylated dimethacrylate (BPAEDMA)] processed into electrospun nanofibrous scaffolds. The composition, morphology and mechanical properties of the compound scaffolds, composed of varying ratios of PCL and hydrogel were characterized by scanning electron microscopy, infrared spectroscopy and dynamic mechanical analyzer. The elastic modulus of PCL/BPAEDMA nanofibrous scaffolds was shown to be varying the BPAEDMA weight fraction and was decreased by increasing the BPAEDMA weight fraction. Compound fiber meshes containing 75 wt % BPAEDMA oligomer hydrogel exhibited lower modulus (3.55 MPa) and contact angle of 25(o) . Rabbit cardiac cells cultured for 10 days on these PCL/BPAEDMA compound nanofibrous scaffolds remained viable and expressed cardiac troponin and alpha-actinin proteins for the normal functioning of myocardium. Cell adhesion and proliferations were significantly increased on compound fiber meshes containing 75 wt % BPAEDMA, when compared with other nanofibrous scaffolds. The results observed that the produced PCL/BPAEDMA compound nanofibrous scaffolds promote cell adhesion, proliferation and normal functioning of cardiac cells to clinically beneficial levels, relevant for cardiac tissue engineering.
“…Candidate substrate materials must exhibit biocompatibility, while supporting cell proliferation, which is hypothesized to be a direct function of its stiffness. Bioengineered scaffold stiffness must be closely resemble that of native ECM and flexible to allow the contraction of growing cells . In the case of PCL membranes, 3T3 fibroblast cell proliferation was most significant on low stiffness samples .…”
Polycaprolactone (PCL), a synthetic biocompatible and biodegradable polymer generally used as a scaffold material for tissue engineering applications. The high stiffness and hydrophobicity of the PCL fiber mesh does not provide significant cell attachment and proliferation in cardiac tissue engineering. Towards this goal, the study focused on a compound of PCL and oligomer hydrogel [Bisphenol A ethoxylated dimethacrylate (BPAEDMA)] processed into electrospun nanofibrous scaffolds. The composition, morphology and mechanical properties of the compound scaffolds, composed of varying ratios of PCL and hydrogel were characterized by scanning electron microscopy, infrared spectroscopy and dynamic mechanical analyzer. The elastic modulus of PCL/BPAEDMA nanofibrous scaffolds was shown to be varying the BPAEDMA weight fraction and was decreased by increasing the BPAEDMA weight fraction. Compound fiber meshes containing 75 wt % BPAEDMA oligomer hydrogel exhibited lower modulus (3.55 MPa) and contact angle of 25(o) . Rabbit cardiac cells cultured for 10 days on these PCL/BPAEDMA compound nanofibrous scaffolds remained viable and expressed cardiac troponin and alpha-actinin proteins for the normal functioning of myocardium. Cell adhesion and proliferations were significantly increased on compound fiber meshes containing 75 wt % BPAEDMA, when compared with other nanofibrous scaffolds. The results observed that the produced PCL/BPAEDMA compound nanofibrous scaffolds promote cell adhesion, proliferation and normal functioning of cardiac cells to clinically beneficial levels, relevant for cardiac tissue engineering.
“…They should (1) have compliant mechanical properties with native tissue, (2) be biodegradable, (3) have a pore size and interconnectivity favorable to the establishment of vascular networks, colonization by cardiomyocytes or progenitors, and facilitate oxygen and nutrients diffusion, (4) promote cellular adhesion (e.g., peptide sequence RGD in hydrogels), and (5) allow the incorporation of bioactive molecules to be released with specific kinetics. Examples of materials already tested in MI therapy include natural (e.g., collagen, fibrin, hyaluronan, alginate) or synthetic (e.g., polyethylene glycol (PEG) hydrogels; poly (glycerol sebacate), polyester urethane urea elastomers) materials [78]. These biomaterials can be envisioned as the first generation biomaterials for cardiac tissue engineering (Fig.…”
Section: Biomaterials As Sensors and Effectors At The Cardiac Environmentioning
Recent pre-clinical and clinical studies indicate that certain exogenous stem cells and biomaterials can preserve cardiac tissue after myocardial infarction. Regarding stem cells, a growing body of data suggests that the short-term positive outcomes are mainly attributed to paracrine signaling mechanisms. The release of such factors is due to the cell’s ability to sense cardiac environmentally derived cues, though the exact feedback loops are still poorly understood. However, given the limited engraftment and survival of transplanted cells in the ischemic environment, the long-term clinical benefits of these therapies have not yet been realized. To overcome this, the long-term controlled delivery of bioactive factors using biomaterials is a promising approach. A major challenge has been the ability to develop timely and spatially controlled gradients of different cues, pivotal for the development and regeneration of tissues. In addition, given the complexity of the remodeling process after myocardial infarction, multiple factors may be required at distinct disease stages to maximize therapeutic outcomes. Therefore, novel smart materials that can sense the surrounding environment and generate cues through on demand mechanisms will be of major importance in the translation of these promising advanced therapies. This article reviews how the cardiac environment can mediate the release profiles of bioactive cues from cells and biomaterials and how the controlled delivery impacts heart regeneration.
“…The myocardial tissue has poor capability to regenerate itself. Consequently, infarcted area is replaced by fibrous scar tissue (Castilho et al, ; Mukherjee, Venugopal, Ravichandran, Ramakrishna, & Raghunath, ). Owing to shortage of heart donors, it is necessary to develop new approaches for regeneration of the injured myocardium.…”
Conductive nanofibers have been considered as one of the most interesting and promising candidate scaffolds for cardiac patch applications with capability to improve cell–cell communication. Here, we successfully fabricated electroconductive nanofibrous patches by simultaneous electrospray of multiwalled carbon nanotubes (MWCNTs) on polyurethane nanofibers. A series of CNT/PU nanocomposites with different weight ratios (2:10, 3:10, and 6:10wt%) were obtained. Scanning electron microscopy, conductivity analysis, water contact angle measurements, and tensile tests were used to characterize the scaffolds. FESEM showed that CNTs were adhered on PU nanofibers and created an interconnected web‐like structures. The SEM images also revealed that the diameters of nanofibers were decreased by increasing CNTs. The electrical conductivity, tensile strength, Young's modulus, and hydrophilicity of CNT/PU nanocomposites also enhanced after adding CNTs. The scaffolds revealed suitable cytocompatibility for H9c2 cells and human umbilical vein endothelial cells (HUVECs). This study indicated that simultaneous electrospinning and electrospray can be used to fabricate conductive CNT/PUnanofibers, resulting in better cytocompatibility and improved interactions between the scaffold and cardiomyoblasts.
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