Multimethod combination for age estimation of Sarcophaga peregrina (Diptera: Sarcophagidae) with implications for estimation of the postmortem interval

Shang, Yanjie; Amendt, Jens; Wang, Yu; Ren, Lipin; Yang, Fengqin; Zhang, Xiangyan; Zhang, Changquan; Guo, Yadong

doi:10.1007/s00414-022-02934-7

Cited by 14 publications

(19 citation statements)

References 47 publications

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“…Shang et al (2023) reaffirm the findings of Hall and Martín-Vega (2019) and Fraenkel and Bhaskaran (1973) who supported the present study with histological analyses of M. domesti ca. Shang et al (2023) used different methodologies to estimate the pupal age of Sarcophaga peregrina (Robineau-Desvoidy, 1830), which is a species of the family Sarcophagidae of forensic importance. The temperatures used were 15, 20, 25, and 30 °C and the emergence of adults approximately 1,090.3 ± 30.6, 566.6 ± 21.9, 404.6 ± 13.01, and 280.3 ± 4.5 hr, respectively (Table 2).…”

Section: Discussionsupporting

confidence: 89%

“…The intrapuparial development was divided into 12 substages, 6 associated with external morphology using light microscopy and 10 associated, also, with the internal morphology of the pupae, by histological analysis, as previously stated for both Hall and Martín-Vega (2019) and Fraenkel and Bhaskaran (1973) studies, which subsidized the present one, using histology as an important complementary method to determine intrapuparial development phases. In addition, Shang et al (2023) also used gene expression and found that 6 genes (NDUFS2, CPAMD8, NDUFV2, Hsp27, Hsp23, and TPP) can be considered to estimate the pupal age of S. peregrina .…”

Section: Discussionmentioning

confidence: 99%

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Characterization of intrapuparial development of Musca domestica (Diptera: Muscidae), under different temperatures in laboratory conditions

do Couto,

Queiroz

2023

Journal of Medical Entomology

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Musca domestica (Linnaeus, 1758) (Diptera: Muscidae), popularly known as “housefly”, is a highly synanthropic species, with economic, medical-sanitary, veterinary, and forensic importance. It is able to cause damage to health, transmit pathogenic agents, cause infection in domestic animals, and, in its immature stage, cause secondary myiasis. The scavenging habit of its immature stages makes these flies pioneers in colonizing both human and animal carcasses, from the initial stages of corpse decomposition to the final stages. Intrapuparial development studies of all stages of the biological cycle of these insects help estimate pupal age, being useful to forensic entomology to aid in determining the minimum postmortem interval (minPMI). This study describes, morphologically, the external structures of the pupae, under temperatures of 23, 27, and 30 ± 1 °C aiming to identify the characteristics that define their developmental stages and estimation of the pupae age of M. domestica. The whole experiment was carried out under laboratory conditions, with relative humidity 60 ± 10% and 12 hours of photoperiod. The process of pupariation and pupation including pre-pupae phases were observed; larvae pupae apolysis; early cryptocephalic pupae; late cryptocephalic pupae; phanerocephalic pupae; pharate adult, discriminated by eye color (transparent eyes, pink eyes, and red eyes); and the emergency of adults, which occurred in the intervals of 162–180; 138–144, and 96–102 hr, respectively, being described throughout the metamorphosis of the external morphological characteristics of the pupal stage of M. domestica.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Characterization of intrapuparial development of Musca domestica (Diptera: Muscidae), under different temperatures in laboratory conditions

do Couto,

Queiroz

2023

Journal of Medical Entomology

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“…Shang et al reported a case in which light brown to dark brown pupae were found on the corpse and the death scene. This indicates that the accurate determination of the pupal age is a crucial task for using necrophagous flies to estimate PMImin [ 37 ]. Traditional methods, such as length and weight measurements used to determine the age of larvae, cannot be applied because the length of a pupa is stable, and the weight of a pupa is a very variable factor [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Pupal Age Estimation of Sarcophaga peregrina (Diptera: Sarcophagidae) at Different Constant Temperatures Utilizing ATR-FTIR Spectroscopy and Cuticular Hydrocarbons

Shang

Feng

Ren

et al. 2023

Insects

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Sarcophaga peregrina (Robineau-Desvoidy, 1830) (Diptera: Sarcophagidae) is a forensically important flesh fly that has potential value in estimating the PMImin. The precise pupal age estimation has great implications for PMImin estimation. During larval development, the age determination is straightforward by the morphological changes and variation of length and weight, however, the pupal age estimation is more difficult due to anatomical and morphological changes not being visible. Thus, it is necessary to find new techniques and methods that can be implemented by standard experiments for accurate pupal age estimation. In this study, we first investigated the potential of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and cuticular hydrocarbons (CHCs) for the age estimations of S. peregrina pupae at different constant temperatures (20 °C, 25 °C, and 30 °C). The orthogonal projections latent structure discrimination analysis (OPLS-DA) classification model was used to distinguish the pupae samples of different developmental ages. Then, a multivariate statistical regression model, partial least squares (PLS), was established with the spectroscopic and hydrocarbon data for pupal age estimations. We identified 37 CHCs with a carbon chain length between 11 and 35 in the pupae of S. peregrina. The results of the OPLS-DA model show a significant separation between different developmental ages of pupae (R2X > 0.928, R2Y > 0.899, Q2 > 0.863). The PLS model had a satisfactory prediction with a good fit between the actual and predicted ages of the pupae (R2 > 0.927, RMSECV < 1.268). The results demonstrate that the variation tendencies of spectroscopy and hydrocarbons were time-dependent, and ATR-FTIR and CHCs may be optimal for the age estimations of pupae of forensically important flies with implications for PMImin estimation in forensic practice.

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“…All of these models could be used to estimate the PMImin; however, the biology of the insects and the climatic parameters under which they are dynamic should be considered [37]. In addition, to provide an accurate PMI, Shang et al [28] suggested the use of a multimethod combination, as well as a combination of multidisciplinary specialists, to analyze and interpret data from forensic investigations [95]. Therefore, further research is still required to test other methods using this species.…”

Section: Discussionmentioning

confidence: 99%

“…Most of the species belonging to the Sarcophagidae family exhibit a slow growth rate at low temperatures and a fast growth rate at high temperatures, as evidenced by the development patterns exhibited by Sarcophaga dux Thomson, 1869 (Diptera: Sarcophagidae) [27], Sarcophaga peregrina (Robineau-Desvoidy, 1830) (Diptera: Sarcophagidae) [28], Sarcophaga (Liopygia) argyrostoma Robineau-Desvoidy, 1830 [29], Sarcophaga ruficornis (Fabricius, 1794) [30,31], and Wohlfahrtia nubia Wiedemann, 1830 (Diptera: Sarcophagidae) [32]. Sarcophaga africa had a slow life cycle, with a 1.66-day difference between the rainy and winter seasons and a rapid one during the summer [33].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Influence of Changing and Fixed Temperatures on the Growth and Pteridine Content in the Head of Adults Sarcophaga crassipalpis (Diptera: Sarcophagidae)

Ngando

Zhang

et al. 2023

Animals

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Flesh flies (Diptera: Sarcophagidae) are regarded as significant in medical and veterinary entomology, and their development models can be utilized as considerable markers to ascertain the minimum postmortem interval (PMImin). In this research, we explored the growth cycle and larval body length of Sarcophaga crassipalpis Macquart 1839 (Diptera: Sarcophagidae) reared under variable temperatures ranging from 15.7 to 31.1 °C, with an average of 24.55 °C and relative humidity ranges from 31.4 to 82.8% and at six fixed temperatures of 15, 20, 25, 30, 32, and then 35 °C. Moreover, pteridine from the head was used to assess adult age grading. Our results allowed us to provide three development models: the isomorphen chart, the isomegalen chart, and the thermal summation models. The time taken for S. crassipalpis to complete its development from larviposition to adult emergence at constant temperatures of 15, 20, 25, 30, 32, and 35 °C was 1256.3 ± 124.2, 698.6 ± 15.1, 481.8 ± 35.7, 366.0 ± 13.5, and 295.8 ± 20.5 h, respectively, except 35 °C, where all pupae were unable to attain adulthood. They lasted 485.8 ± 5.4 h under variable temperatures. The minimum developmental limit (D0) temperature and the thermal summation constant (K) of S. crassipalpis were 9.31 ± 0.55 °C and 7290.0 ± 388.4 degree hours, respectively. The increase in pteridine content exhibited variations across different temperatures. There was quite a considerable distinction in the pteridine contents of male and female S. crassipalpis at 15 °C (p = 0.0075) and 25 °C (p = 0.0213). At 32 °C and variable temperatures, the pteridine content between female and male S. crassipalpis was not statistically divergent. However, temperature and gender remain the main factors influencing the pteridine content in the head of S. crassipalpis. We aim to provide detailed developmental data on S. crassipalpis that can be used as a valuable resource for future research and PMI estimation.

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Multimethod combination for age estimation of Sarcophaga peregrina (Diptera: Sarcophagidae) with implications for estimation of the postmortem interval

Cited by 14 publications

References 47 publications

Characterization of intrapuparial development of Musca domestica (Diptera: Muscidae), under different temperatures in laboratory conditions

Characterization of intrapuparial development of Musca domestica (Diptera: Muscidae), under different temperatures in laboratory conditions

Pupal Age Estimation of Sarcophaga peregrina (Diptera: Sarcophagidae) at Different Constant Temperatures Utilizing ATR-FTIR Spectroscopy and Cuticular Hydrocarbons

Analysis of the Influence of Changing and Fixed Temperatures on the Growth and Pteridine Content in the Head of Adults Sarcophaga crassipalpis (Diptera: Sarcophagidae)

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