Multimeric Near IR–MR Contrast Agent for Multimodal <i>In Vivo</i> Imaging

Harrison, Victoria S. R.; Carney, Christiane E.; MacRenaris, Keith W.; Waters, Emily A.; Meade, Thomas J.

doi:10.1021/jacs.5b04509

Cited by 94 publications

(92 citation statements)

References 49 publications

(91 reference statements)

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“…The detection limit of cells labeled with Gd(III) by MR imaging is known to be approximately 0.1 fmol/cell in vitro and 1 fmol/cell in vivo. 50,51 …”

Section: Resultsmentioning

confidence: 99%

Nanodiamond–Gadolinium(III) Aggregates for Tracking Cancer Growth In Vivo at High Field

et al. 2016

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The ability to track labeled cancer cells in vivo would allow researchers to study their distribution, growth, and metastatic potential within the intact organism. Magnetic resonance (MR) imaging is invaluable for tracking cancer cells in vivo as it benefits from high spatial resolution and the absence of ionizing radiation. However, many MR contrast agents (CAs) required to label cells either do not significantly accumulate in cells or are not biologically compatible for translational studies. We have developed carbon-based nanodiamond–gadolinium(III) aggregates (NDG) for MR imaging that demonstrated remarkable properties for cell tracking in vivo. First, NDG had high relaxivity independent of field strength, a finding unprecedented for gadolinium(III) [Gd(III)]–nanoparticle conjugates. Second, NDG demonstrated a 300-fold increase in the cellular delivery of Gd(III) compared to that of clinical Gd(III) chelates without sacrificing biocompatibility. Further, we were able to monitor the tumor growth of NDG-labeled flank tumors by T1- and T2-weighted MR imaging for 26 days in vivo, longer than was reported for other MR CAs or nuclear agents. Finally, by utilizing quantitative maps of relaxation times, we were able to describe tumor morphology and heterogeneity (corroborated by histological analysis), which would not be possible with competing molecular imaging modalities.

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“…The detection limit of cells labeled with Gd(III) by MR imaging is known to be approximately 0.1 fmol/cell in vitro and 1 fmol/cell in vivo. 50,51 …”

Section: Resultsmentioning

confidence: 99%

Nanodiamond–Gadolinium(III) Aggregates for Tracking Cancer Growth In Vivo at High Field

et al. 2016

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“…65 Accumulation of these agents in a nude mouse MCF7 tumor xenograft model was detected with fluorescence imaging, and renal clearance by MR imaging. 24 …”

Section: Tumor Xenograft Mouse Modelsmentioning

confidence: 99%

Review on near-infrared heptamethine cyanine dyes as theranostic agents for tumor imaging, targeting, and photodynamic therapy

2016

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Review on near-infrared heptamethine cyanine dyes as theranostic agents for tumor imaging, targeting, and photodynamic therapy," J. Biomed. Opt. 21(5), 050901 (2016), doi: 10.1117/1.JBO.21.5.050901. Abstract. A class of near-infrared fluorescence (NIRF) heptamethine cyanine dyes that are taken up and accumulated specifically in cancer cells without chemical conjugation have recently emerged as promising tools for tumor imaging and targeting. In addition to their fluorescence and nuclear imagingbased tumor-imaging properties, these dyes can be developed as drug carriers to safely deliver chemotherapy drugs to tumors. They can also be used as effective agents for photodynamic therapy with remarkable tumoricidal activity via photodependent cytotoxic activity. The preferential uptake of dyes into cancer but not normal cells is co-operatively mediated by the prevailing activation of a group of organic aniontransporting polypeptides on cancer cell membranes, as well as tumor hypoxia and increased mitochondrial membrane potential in cancer cells. Such mechanistic explorations have greatly advanced the current application and future development of NIRF dyes and their derivatives as anticancer theranostic agents. This review summarizes current knowledge and emerging advances in NIRF dyes, including molecular characterization, photophysical properties, multimodal development and uptake mechanisms, and their growing potential for preclinical and clinical use.

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“…4H). In addition to MRI, the amount of Gd(III) label can also be quantified with great sensitivity using inductively coupled plasma mass spectrometry (ICP-MS) because of the negligible endogenous concentration of Gd(III) (41). Hence, we injected TA muscles of mice with Gd(III)-labeled scaffolds (Fig.…”

Section: Development Of An Injection Apparatus To Form Aligned Biomimmentioning

confidence: 99%

Injectable biomimetic liquid crystalline scaffolds enhance muscle stem cell transplantation

Sleep

Cosgrove

McClendon

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Muscle stem cells are a potent cell population dedicated to efficacious skeletal muscle regeneration, but their therapeutic utility is currently limited by mode of delivery. We developed a cell delivery strategy based on a supramolecular liquid crystal formed by peptide amphiphiles (PAs) that encapsulates cells and growth factors within a muscle-like unidirectionally ordered environment of nanofibers. The stiffness of the PA scaffolds, dependent on amino acid sequence, was found to determine the macroscopic degree of cell alignment templated by the nanofibers in vitro. Furthermore, these PA scaffolds support myogenic progenitor cell survival and proliferation and they can be optimized to induce cell differentiation and maturation. We engineered an in vivo delivery system to assemble scaffolds by injection of a PA solution that enabled coalignment of scaffold nanofibers with endogenous myofibers. These scaffolds locally retained growth factors, displayed degradation rates matching the time course of muscle tissue regeneration, and markedly enhanced the engraftment of muscle stem cells in injured and noninjured muscles in mice.biomaterials | scaffold | muscle stem cell | cell delivery | muscle regeneration

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Multimeric Near IR–MR Contrast Agent for Multimodal In Vivo Imaging

Cited by 94 publications

References 49 publications

Nanodiamond–Gadolinium(III) Aggregates for Tracking Cancer Growth In Vivo at High Field

Nanodiamond–Gadolinium(III) Aggregates for Tracking Cancer Growth In Vivo at High Field

Review on near-infrared heptamethine cyanine dyes as theranostic agents for tumor imaging, targeting, and photodynamic therapy

Injectable biomimetic liquid crystalline scaffolds enhance muscle stem cell transplantation

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