The understanding of knowledge transfer and information delivery is recently in the broader scope due to the development of educational technology. The information delivery is not merely done using verbal message; however, multiple modes of presentation such as verbal and auditory representation can also be the alternative of material delivery. The studies featuring the use of multimedia-based presentation are mostly administered to determine its effectiveness to be implemented in the learning process. In contrast, the exploration of the use of multimedia-based presentation toward the way how it can be a means of interaction seems underexplored. Therefore, to make it be more precise, the present study attempts to explore the practice of multimedia-based presentation in academic speaking classroom and to investigate the interactivity emerged during the presentation process. This study deployed qualitative case study design due to the purpose of gaining the in-depth investigation of the use of multimediabased presentation and its interactivity emergence in academic speaking classroom. The study was conducted in one of the universities in Surakarta majoring English Education where academic speaking becomes one of the subjects. The presentation document along with the presentation process were analyzed in this study. The results of the analysis show that multimedia-based presentation is designed to visualize the material being conveyed through the icons, pictures, and illustrations that are able to represent the information or knowledge in a more concrete way. Moreover, the interactivity is also emerged through the use of multimedia-based presentation as it simplifies the presenter to do the following: gesturing, dialoguing, and describing. The results implies the opportunity for both teachers and academic speaking presenters to innovate how they present the material by using multimedia-based presentation. In practice, multimedia-based presentation along with its interactivity can clarify the materials, grab the audience attention, and stimulate the audience responses.