Multilocus sequence typing of Mycoplasma mycoides subsp. capri to assess its genetic variability in a contagious agalactia endemic area

Tatay-Dualde, Juan; Ham, Miranda Prats-van der; Fe, Christian de la; Gómez-Martín, Ángel; Paterna, A.; Corrales, J.C.; Contreras, A.; Sánchez, Antonio

doi:10.1016/j.vetmic.2016.06.002

Cited by 10 publications

(14 citation statements)

References 19 publications

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“…Since previous studies reported phylogenetic differences between isolates coming from the same farms [16, 17], our study included the study of three farms from which more than one isolate were retrieved. Phylogenetic differences appeared between different isolates coming from one of these farms, but only when isolates were retrieved at different years.…”

Section: Resultsmentioning

confidence: 99%

“…In this context, the genetic variability showed by Mcc at the present work is in concordance with a prior work with Mycoplasma mycoides subsp. capri [17] which is closely related to Mcc and also reported a substantial genetic diversity. This fact could be explained by the proved genetic transmission which happens amongst different mycoplasma species when they coinfect a single host [36].…”

Section: Discussionmentioning

confidence: 99%

“…This technique utilizes sequences of internal fragments from housekeeping genes in order to characterize bacterial isolates, and it seems to be the most appropriate technique to analyze evolutionary relationships amongst mycoplasma isolates [13–16]. Hence, in previous studies with other mycoplasmas, a great genetic diversity between isolates from the same area (country) [13, 17] or even from the same farm [16] was observed. As for the members of the M .…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Tatay-Dualde

Ham

et al. 2017

PLoS ONE

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Mycoplasma capricolum subsp. capricolum is one of the causative agents of contagious agalactia (CA). Nevertheless, there is still a lack of information about its antimicrobial susceptibility and genetic characteristics. Therefore, the aim of this work was to study the antimicrobial and genetic variability of different Mycoplasma capricolum subsp. capricolum field isolates. For this purpose, the growth inhibition effect of 18 antimicrobials and a multilocus sequence typing (MLST) scheme based on five housekeeping genes (fusA, glpQ, gyrB, lepA and rpoB) were performed on 32 selected field isolates from Italy and Spain.The results showed a wide range of growth inhibitory effects for almost all the antimicrobials studied. Macrolides presented lower efficacy inhibiting Mcc growth than in previous works performed on other CA-causative mycoplasmas. Erythromycin was not able to inhibit the growth of any of the studied strains, contrary to doxycycline, which inhibited the growth of all of them from low concentrations. On the other hand, the study of the concatenated genes revealed a high genetic variability among the different Mcc isolates. Hence, these genetic variations were greater than the ones reported in prior works on other mycoplasma species.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Tatay-Dualde

Ham

et al. 2017

PLoS ONE

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“…Molecular pathogenesis of Mycoplasma spp, associated to a high and unexpected genetic variability mediated by mutation (i.e., in housekeeping genes) and recombination (i.e., in genes subjected to selective pressure) mechanisms, is increasingly better known in small ruminants (Flitman-Tene et al, 2003;McAuliffe et al, 2011;Tatay-Dualde et al, 2016) and genetically engineered DNA vaccines against specific recombinant mechanisms could be developed in the near future.…”

Section: Mycoplasma Sppmentioning

confidence: 99%

Milk hygiene in small ruminants: A review

Gonzalo

2018

Span. j. agric. res.

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Somatic cell count (SCC), mammary pathogens prevalence, total and specific bacterial counts, antimicrobial residues, macroscopic sediment, water addition, aflatoxins and other contaminants constitute the basis for milk payment-schemes, monitoring and improvement of flock hygiene and health management, and development of analytical surveillance programs in the dairy small ruminants. The present work reviews factors influencing the variation of these variables, including milk analytical methods, storage and preservation, along with management implications during the last two decades. Following farmer and cooperative educational programs, progressive reductions have been reported for total bacterial count and antimicrobial residue occurrence in bulk tank milk. These results were consistent, however, with high values for SCC and specific bacterial populations. Thus, mastitis control programs should be intensified to increase hygiene in milk and economic returns for producers and processors. In addition, the implementation of programs to reduce specific bacterial counts (i.e., psychrotrophs, coliforms, Clostridium spp. spores) and mammary pathogen prevalence (i.e., Staph. aureus, Mycoplasma spp.), as well as the use of combined screening methods for an increased rate of antimicrobial detection, are currently required strategies which are positively valuated by milk processors, industry and consumers. Other contaminants may also be present, but cost-effective screening and analytical systems have not yet been implemented. This review aims to be helpful for troubleshooting milk quality and safety, developing future premium payment systems and industry quality-standards, optimizing management, on-farm risk traceability systems and consumer acceptance.

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“…Hence for better diagnosis, laboratory diagnosis is important of which isolation and identification is considered as gold standard method of diagnosis (Thiaucourt & Bolske, 1996;DaMassa, 1996;Nicholas, 2002;Chakraborty et al, 2014). Laboratory diagnosis involves an efficient sampling protocol, expensive but highly specific cultural media and culturing techniques, isolation in a well-equipped and safe laboratory, identification and characterization by specific and sensitive methods involving colony characteristics, staining, morphology, biochemical testing, agglutination, complement fixation, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), and genome sequencing (Manso-Silván et al, 2011;Chakraborty et al, 2014;TatayDualde et al, 2015;Tatay-Dualde et al, 2016). A combined group of tests can be applied together (multiplex PCR) (Manso-Silván et al, 2011;Chakraborty et al, 2014;Prats-van der Ham et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Mycoplasmosis in Small Ruminants in India: A Review

Yatoo¹,

Kashmir²,

Parray³

et al. 2018

JEBAS

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Mycoplasmosis, the diseases caused by mycoplasma, are one of the challenging and continuous threats to small ruminant farming. They cause heavy morbidity (upto 100%), mortality (10-100%) and huge economic loss. Common diseases caused by mycoplasma organisms are contagious caprine pleuropneumonia (mostly in goats), contagious agalactia (both sheep and goats), atypical pneumonia (usually sheep), besides arthritis, mastitis, seminal vesiculitis, ampullitis, epididymitis, orchitis, urethritis, conjunctivitis and meningitis either alone or as classical syndromes. The common mycoplasma includes Mycoplasma mycoides subspecies mycoides, M. mycoides subsp. capri, M. capricolum subsp. capripneumoniae, M. capricolum subsp. capricolum, M. agalactiae, M. bovis and M. conjunctivae etc. Most of the mycoplasmas produce a conglomerate of clinical signs hampering diagnosis. The other main challenge posed by mycoplasma is the difficulty in isolation due to lack of

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Multilocus sequence typing of Mycoplasma mycoides subsp. capri to assess its genetic variability in a contagious agalactia endemic area

Cited by 10 publications

References 19 publications

Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Milk hygiene in small ruminants: A review

Mycoplasmosis in Small Ruminants in India: A Review

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