Multilocus Sequence Typing of
            <i>Neisseria meningitidis</i>
            Directly from Clinical Samples and Application of the Method to the Investigation of Meningococcal Disease Case Clusters

Birtles, Andrew; Hardy, Katie; Gray, Stephen J.; Handford, S; Kaczmarski, Edward B.; Edwards-Jones, Valerie

doi:10.1128/jcm.43.12.6007-6014.2005

Cited by 35 publications

(22 citation statements)

References 42 publications

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“…PCR and Sanger sequencing were used for the remaining 28 DNAs obtained from culture-negative samples to determine ST, cc, porA and fetA (Birtles et al, 2005;Thompson et al, 2003). Briefly, for fetA gene amplification and sequencing, oligonucleotides described by Thompson et al (2003) were used.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of a collection of Neisseria meningitidis isolates from Croatia, June 2009 to January 2014

Bukovski¹,

Vacca

Anselmo³

et al. 2016

Journal of Medical Microbiology

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In the last decade, the incidence of invasive meningococcal disease (IMD) in Croatia remained stable at approximately 1 case per 100 000 inhabitants, affecting mainly children aged 5 years. We report the molecular characterization of meningococci causing IMD occurring from June 2009 to January 2014 in Croatia. Genomic DNA from 50 clinical isolates was analysed for serogroup, multilocus sequence typing and allele type of the two outer membrane protein genes, porA and the iron-regulated fetA. Furthermore, 22 of them were characterized by using whole-genome sequencing to define the meningococcal vaccine four-component meningococcal serogroup B (4CMenB) antigen genes factor H-binding protein (fHbp), Neisseria heparin-binding antigen (nhba) and Neisseria adhesin A (nadA) and the antimicrobial target resistance genes for penicillin (penicillin binding protein 2, penA), ciprofloxacin (DNA gyrase subunit A, gyrA) and rifampicin (b-subunit of RNA polymerase, rpoB). The Etest was used to phenotypically determine the antimicrobial susceptibility of isolated meningococci. The main serogroup/clonal complex combinations were MenB cc41/44, MenC/cc11, MenW/cc174 and MenY/cc23. PorA P1.7-2, FetA F5-5 and F1-5 were the most represented through the serogroups. Meningococci with decreased susceptibility to penicillin (38.9 %) and one strain resistant to ciprofloxacin were identified. Forty-two percent of MenB showed the presence of at least one of the 4CMenB vaccine antigens (fHbp, NHBA, NadA and PorA). Our findings highlight the genetic variability of meningococci causing IMD in Croatia, especially for the serogroup B. Molecular-based characterization of meningococci is crucial to enhance IMD surveillance and to better plan national immunization programmes. INTRODUCTIONInvasive meningococcal disease (IMD) is caused by Neisseria meningitidis, a strict human pathogen affecting mainly children 5 years of age, adolescents and young adults (Stephens, 2009). IMD is characterized by meningitis, sepsis and less commonly pneumonia, arthritis and pericarditis.According to the European Centre for Disease Prevention and Control (ECDC) data, the incidence of IMD reported in European Union (EU) and European Economic Area countries in 2012 was substantially low (ECDC, 2014), with an overall notification rate of 0.68 cases per 100 000 inhabitants. IMD case fatality ranged from 8 to 15 %, and approximately 10-20 % of survivors suffered from long-term sequelae including mental retardation, motor nerve deficit, hearing loss and loss of limbs (ECDC, 2014;Stephens, 2009). †These authors contributed equally to this work.

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Section: Methodsmentioning

confidence: 99%

Molecular characterization of a collection of Neisseria meningitidis isolates from Croatia, June 2009 to January 2014

Bukovski¹,

Vacca

Anselmo³

et al. 2016

Journal of Medical Microbiology

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“…Recently, several techniques have been developed that allow these analyses on various body fluids (e.g., CSF, blood, urine, others) directly, further decreasing the utility of culture [40][41][42][43][44]. A recent study has demonstrated the utility of antigen detection kits, such as latex agglutination for serogroup determination during meningococcal epidemics [45].…”

Section: Serogroup Identificationmentioning

confidence: 99%

Meningococcal serogroup W135 in the African meningitis belt: epidemiology, immunity and vaccines

Mueller¹,

Borrow²,

Gessner³

2006

Expert Review of Vaccines

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In the sub-Saharan African meningitis belt there is a region of hyperendemic and epidemic meningitis stretching from Senegal to Ethiopia. The public health approaches to meningitis epidemics, including those related to vaccine use, have assumed that Neisseria meningitidis serogroup A will cause the most disease. During 2001 and 2002, the first large-scale epidemics of serogroup W135 meningitis in sub-Saharan Africa were reported from Burkina Faso. The occurrence of N. meningitidis W135 epidemics has led to a host of new issues, including the need for improved laboratory diagnostics for identifying serogroups during epidemics, an affordable supply of serogroup W135-containing polysaccharide vaccine for epidemic control where needed, and re-evaluating the long-term strategy of developing a monovalent A conjugate vaccine for the region. This review summarizes the existing data on N. meningitidis W135 epidemiology, immunology and vaccines as they relate to meningitis in sub-Saharan Africa.

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“…The amplification and sequencing of the porA and penA genes was carried out as described in Birtles et al (6) and Taha et al (22), respectively.…”

Section: Methodsmentioning

confidence: 99%

Evolutionary Changes in Antimicrobial Resistance of Invasive Neisseria meningitidis Isolates in Belgium from 2000 to 2010: Increasing Prevalence of Penicillin Nonsusceptibility

Bertrand

Carion

Wintjens

et al. 2012

Antimicrob Agents Chemother

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bThis study was conducted to evaluate the evolution of the antimicrobial susceptibility of Neisseria meningitidis causing invasive diseases in Belgium in the period of January 2000 to December 2010. A total of 1,933 cases of N. meningitidis from invasive infections were analyzed by antimicrobial susceptibility testing at the Belgian Meningococcal Reference Centre. The majority of strains were susceptible to antibiotics that are currently used for the treatment and prophylaxis of meningococcal disease, but the prevalence of clinical isolates with reduced susceptibility to penicillin increased over the years. The phenotyping, genotyping, and determination of MICs of penicillin G were performed. The systematic shift of the curves toward higher penicillin MICs in the susceptible population indicated that this population became less sensitive to penicillin in this period. A 402-bp DNA fragment in the 3= end of penA was sequenced for the 296 nonsusceptible meningococcal strains isolated between 2000 and 2010 to examine the genetic diversity and evolution of their penA gene. In conclusion, the data obtained in our study support the statement that the position of penicillin G as a first choice in the treatment of invasive meningococcal diseases in Belgium should be reexamined. Despite an important number of isolates displaying a reduced susceptibility to penicillin, at present the expandedspectrum cephalosporins, such as ceftriaxone, are not affected. The follow-up of the evolutionary changes in antimicrobial resistance has also proved to be essential for the recommendation of an appropriate antimicrobial treatment for invasive meningococcal diseases.

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Multilocus Sequence Typing of Neisseria meningitidis Directly from Clinical Samples and Application of the Method to the Investigation of Meningococcal Disease Case Clusters

Cited by 35 publications

References 42 publications

Molecular characterization of a collection of Neisseria meningitidis isolates from Croatia, June 2009 to January 2014

Molecular characterization of a collection of Neisseria meningitidis isolates from Croatia, June 2009 to January 2014

Meningococcal serogroup W135 in the African meningitis belt: epidemiology, immunity and vaccines

Evolutionary Changes in Antimicrobial Resistance of Invasive Neisseria meningitidis Isolates in Belgium from 2000 to 2010: Increasing Prevalence of Penicillin Nonsusceptibility

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