Multilocus sequence typing of <i>Campylobacter jejuni</i>
 and <i>Campylobacter coli</i>
 isolates from poultry, cattle and humans in Nigeria

Ngulukun, Sati Samuel; Oboegbulem, S. I.; Klein, Günter

doi:10.1111/jam.13185

Cited by 18 publications

(18 citation statements)

References 26 publications

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“…Additionally, a Canadian study reported CC-353 in C. jejuni isolates from chickens originated in Peru, Bolivia and Argentina (Lévesque et al, 2008 ). Other less common CCs found in this study (CC-45, CC-48, CC-52, CC-460, CC-658,CC-464, and CC-607) have also been reported in poultry from Europe, Africa, Asia, and North America (Lévesque et al, 2008 ; Griekspoor et al, 2010 ; O’Mahony et al, 2011 ; Kittl et al, 2013 ; Ngulukun et al, 2016 ; Zeng et al, 2016 ).…”

Section: Discussionsupporting

confidence: 72%

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Vinueza-Burgos

Wautier²,

Martiny

et al. 2017

Poultry Science

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Thermotolerant Campylobacter spp. are a major cause of foodborne gastrointestinal infections worldwide. The linkage of human campylobacteriosis and poultry has been widely described. In this study we aimed to investigate the prevalence, antimicrobial resistance and genetic diversity of C. coli and C. jejuni in broilers from Ecuador. Caecal content from 379 randomly selected broiler batches originating from 115 farms were collected from 6 slaughterhouses located in the province of Pichincha during 1 year. Microbiological isolation was performed by direct plating on mCCDA agar. Identification of Campylobacter species was done by PCR. Minimum inhibitory concentration (MIC) values for gentamicin, ciprofloxacin, nalidixic acid, tetracycline, streptomycin, and erythromycin were obtained. Genetic variation was assessed by RFLP-flaA typing and Multilocus Sequence Typing (MLST) of selected isolates. Prevalence at batch level was 64.1%. Of the positive batches 68.7% were positive for C. coli, 18.9% for C. jejuni, and 12.4% for C. coli and C. jejuni. Resistance rates above 67% were shown for tetracycline, ciprofloxacin, and nalidixic acid. The resistance pattern tetracycline, ciprofloxin, and nalidixic acid was the dominant one in both Campylobacter species. RFLP-flaA typing analysis showed that C. coli and C. jejuni strains belonged to 38 and 26 profiles respectively. On the other hand MLST typing revealed that C. coli except one strain belonged to CC-828, while C. jejuni except 2 strains belonged to 12 assigned clonal complexes (CCs). Furthermore 4 new sequence types (STs) for both species were described, whereby 2 new STs for C. coli were based on new allele sequences. Further research is necessary to estimate the impact of the slaughter of Campylobacter positive broiler batches on the contamination level of carcasses in slaughterhouses and at retail in Ecuador.

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Section: Discussionsupporting

confidence: 72%

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Vinueza-Burgos

Wautier²,

Martiny

et al. 2017

Poultry Science

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“…In contrast to the profusion of poultry-associated lineages, there was a striking paucity of host generalist ST-21 and ST-45 clonal complexes [40] that are among the most common diseasecausing lineages in Europe and North America. This has previously been observed in another LMICs, with very few ST-21 complex isolates cultured in surveys from Africa, SE Asia and South America [84][85][86][87][88]. Ruminant specialist lineages were also rare among the Peruvian pediatric samples (6.1 %) and the most common cattle associated lineage (ST-61 complex [25]) was completely absent.…”

Section: Poultry Is the Predominant Source Of Infection In Peruvian Asupporting

confidence: 63%

Genomic epidemiology ofCampylobacter jejuniassociated with asymptomatic pediatric infection in the Peruvian Amazon

Pascoe

Schiaffino

Murray

et al. 2020

Preprint

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Campylobacter is the leading bacterial cause of gastroenteritis worldwide and its incidence is especially high in low- and middle-income countries (LMIC). Disease epidemiology in LMICs is different compared to high income countries like the USA or in Europe. Children in LMICs commonly have repeated and chronic infections even in the absence of symptoms, which can lead to deficits in early childhood development. In this study, we sequenced and characterized C. jejuni (n=62) from a longitudinal cohort study of children under the age of 5 with and without diarrheal symptoms, and contextualized them within a global C. jejuni genome collection. Epidemiological differences in disease presentation were reflected in the genomes, specifically by the absence of some of the most common global disease-causing lineages. As in many other countries, poultry-associated strains were a major source of human infection but almost half of local disease cases (15 of 31) were attributable to genotypes that are rare outside of Peru. Asymptomatic infection was not limited to a single (or few) human adapted lineages but resulted from phylogenetically divergent strains suggesting an important role for host factors in the cryptic epidemiology of campylobacteriosis in LMICs.

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“…This was also reflected in the core genomes of the both datasets, despite a smaller alignment fewer NCTC11168 (an ST-21CC isolate) genes were identified in the Peru-only dataset. The absence of ST-21 complex isolates has previously been observed in another LMICs, with very few isolates cultured in surveys from Africa, SE Asia and South America [85][86][87][88][89]. Ruminant specialist lineages were also rare among the Peruvian pediatric samples (6.1%) and the most common cattle associated lineage (ST-61 complex [25]) was completely absent.…”

Section: Discussionmentioning

confidence: 64%

Genomic epidemiology of Campylobacter jejuni associated with asymptomatic pediatric infection in the Peruvian Amazon

et al. 2020

View full text Add to dashboard Cite

Campylobacter is the leading bacterial cause of gastroenteritis worldwide and its incidence is especially high in low-and middle-income countries (LMIC). Disease epidemiology in LMICs is different compared to high income countries like the USA or in Europe. Children in LMICs commonly have repeated and chronic infections even in the absence of symptoms, which can lead to deficits in early childhood development. In this study, we sequenced and characterized C. jejuni (n = 62) from a longitudinal cohort study of children under the age of 5 with and without diarrheal symptoms, and contextualized them within a global C. jejuni genome collection. Epidemiological differences in disease presentation were reflected in the genomes, specifically by the absence of some of the most common global diseasecausing lineages. As in many other countries, poultry-associated strains were likely a major source of human infection but almost half of local disease cases (15 of 31) were attributable to genotypes that are rare outside of Peru. Asymptomatic infection was not limited to a single (or few) human adapted lineages but resulted from phylogenetically divergent strains suggesting an important role for host factors in the cryptic epidemiology of campylobacteriosis in LMICs.

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Multilocus sequence typing of Campylobacter jejuni and Campylobacter coli isolates from poultry, cattle and humans in Nigeria

Cited by 18 publications

References 26 publications

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Genomic epidemiology ofCampylobacter jejuniassociated with asymptomatic pediatric infection in the Peruvian Amazon

Genomic epidemiology of Campylobacter jejuni associated with asymptomatic pediatric infection in the Peruvian Amazon

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