Multilocus Sequence Typing of Hospital-Associated Enterococcus faecium from Brazil Reveals Their Unique Evolutionary History

Titze‐de‐Almeida, Ricardo; Belkum, Alex van; Felipe, Maria Sueli Soares; Zanella, Rosemeire Cobo; Top, Janetta; Willems, Rob J. L.

doi:10.1089/mdr.2006.12.121

Cited by 16 publications

(4 citation statements)

References 25 publications

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“…Of note, all VREfm isolated in this study were resistant to both ampicillin and quinolones, showing the same antimicrobial resistance profile observed for E. faecium of CC-17. Nevertheless, molecular epidemiological studies conducted with Brazilian E. faecium isolates from several hospitals showed that this complex is not common in our country 27,28 .…”

Section: Conflict Of Interest Financial Support Referencesmentioning

confidence: 70%

Trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital: a 4-year study

Conceição

Oliveira

Silva

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction: In the past two decades members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. This study prospectively analyzed the distribution of species and trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital from 2006-2009. Methods: Enterococcal species were identified by conventional biochemical tests. The antimicrobial susceptibility profile was performed by disk diffusion in accordance with the Clinical and Laboratory Standards Institute (CLSI). A screening test for vancomycin was also performed. Minimal inhibitory concentration (MIC) for vancomycin was determined using the broth dilution method. Molecular assays were used to confirm speciation and genotype of vancomycin-resistant enterococci (VRE). Results: A total of 324 non-repetitive enterococcal isolates were recovered, of which 87% were E. faecalis and 10.8% E. faecium. The incidence of E. faecium per 1,000 admissions increased significantly (p < 0.001) from 0.3 in 2006 to 2.3 in 2009. The VRE rate also increased over time from 2.5% to 15.5% (p < 0.001). All VRE expressed high-level resistance to vancomycin (MIC >256μg/ mL) and harbored vanA genes. The majority (89.5%) of VRE belonged to E. faecium species, which were characteristically resistant to ampicillin and quinolones. Overall, ampicillin resistance rate increased significantly from 2.5% to 21.4% from 2006-2009. Resistance rates for gentamicin, chloramphenicol, tetracycline, and erythromycin significantly decreased over time, although they remained high. Quinolones resistance rates were high and did not change significantly over time. Conclusions: The data obtained show a significant increasing trend in the incidence of E. faecium resistant to ampicillin and vancomycin.

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Section: Conflict Of Interest Financial Support Referencesmentioning

confidence: 70%

Trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital: a 4-year study

Conceição

Oliveira

Silva

et al. 2011

Rev. Soc. Bras. Med. Trop.

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“…Molecular epidemiological studies have shown that the incidence of outbreaks caused by VRE fm strains in Brazil is not as high as in European countries and in the United States [25,35,45]. Palazzo et al (2011) [35] revealed that VRE fm outbreaks in Brazil are caused by strains that do not share a common evolutionary history.…”

Section: Discussionmentioning

confidence: 99%

Emergence of vanA vancomycin-resistant Enterococcus faecium in a hospital in Porto Alegre, South Brazil

Resende

Caierão

Prates

et al. 2014

J Infect Dev Ctries

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Introduction: In Porto Alegre (South Brazil), since the first VRE isolation in 2000 until the middle of the last decade, the epidemiology of enterococcal infections presented the peculiarity that, as opposed to other regions of the country, almost all VRE were E. faecalis. The aim of this study was to investigate the microbiological and epidemiological characteristics of a VRE outbreak that occurred between August 2010 and September 2011 in Porto Alegre, South Brazil. Methodology: Twenty-nine isolates from inpatients of Mãe de Deus Hospital that were identified and characterized for their susceptibility profile, vancomycin genotype, presence of esp gene, biofilm production, and clonal relationship were collected. Patients' records were reviewed for clinical information. Results: All isolates were identified as vancomycin/ampicillin resistant E. faecium carrying the vanA gene. Almost all were susceptible to gentamicin and streptomycin. Most patients died and were associated with a hemodialysis unit stay. All but the first isolate were clustered in a main clone. Conclusions: An important change in vancomycin-resistant enterococci was observed. Studies like this are necessary to monitor the dissemination of VRE, especially of some individual clones.

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“…In Brazil, vancomycin-resistant Enterococcus faecium and E. faecalis are disseminated in hospitals in different regions 2,3 . Recognition of colonized patients represents a crucial step in controlling the dissemination of these organisms and is a laboratorybased strategy 4 .…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

Cantarelli¹,

Cavalcante²,

Pilger³

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Multilocus Sequence Typing of Hospital-Associated Enterococcus faecium from Brazil Reveals Their Unique Evolutionary History

Cited by 16 publications

References 25 publications

Trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital: a 4-year study

Trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital: a 4-year study

Emergence of vanA vancomycin-resistant Enterococcus faecium in a hospital in Porto Alegre, South Brazil

Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil

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