Multilocus sequence typing analyses of Clostridium perfringens type A strains harboring tpeL and netB genes

Nakano, Viviane; Ignácio, Aline; Llanco, Luis; Bueris, Vanessa; Sircili, Marcelo Palma; Avila-Campos, Mario Júlio

doi:10.1016/j.anaerobe.2017.02.017

Cited by 29 publications

(19 citation statements)

References 35 publications

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“…NetB toxin gene is plasmid coded, pore-forming toxin plays a crucial role in the pathogenesis of necrotic enteritis in broiler (24). The isolates in the present study were found to be negative for netB gene similar to that which have been reported by Nakano et al (25) and Merati et al (26).…”

Section: Multiplex and Uniplex Pcrsupporting

confidence: 88%

Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province

2019

AJVS

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The study is conducting to isolate and toxinotype the suspected cases of Clostridium perfringens infections of broiler farms in Sulaimania province. A total of 108 samples were collected from intestinal contents, mucosal scraping, and hemorrhagic lymphoid nodules from suspected cases of necrotic enteritis in broilers. The result of isolated and identified bacteria were revealed that 63 (58%) out of 108 samples were positive for C. perfringens. The results revealed that the isolates were only positive for alpha and beta2 toxin genes. Phylogenetic and DNA sequence analysis of cpa and cpb2 gene showed that cpa genes were highly identical to isolates from broiler in Iran, poultry stool and broiler in Brazil, and blue calves in Belgium. While cpb2 gene is closely related to the isolates of broiler in Iran, India and isolates of goat in Pakistan. The results indicated that the causative agent of necrotic enteritis in broiler farms in the region was mainly due to C. perfringens type A infection

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Section: Multiplex and Uniplex Pcrsupporting

confidence: 88%

Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province

2019

AJVS

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“…After adding 7 human origin isolates (ST75–ST81 in this study) and 10 NE isolates (ST87–ST94 in this study) donated by Liu et al (2020) , Nakano et al. (2017) , and Hibberd et al.…”

Section: Resultsmentioning

confidence: 99%

“…(2006) divided 132 strains of C. perfringens from different host species and toxinotypes into 80 STs and 3 CCs, with an average allele number of 24.4. Nakano et al. (2017) identified an average of 10.25 alleles, 30 STs, and 3 CCs among 40 strains from children and chicken.…”

Section: Discussionmentioning

confidence: 99%

“…For comparison, 10 strains of C. perfringens sequences from broilers with NE previously analyzed by Hibberd et al. (2011) and Nakano et al. (2017) (ST21, ST27, and ST29 of Nakano's study; ST31, ST32, ST34, and ST39 of Hibberd's study) were also used for MLST in the minimum spanning tree.…”

Section: Methodsmentioning

confidence: 99%

“…The detection of virulence genes is an important supplement to the evaluation of population phylogenetic characteristics ( Nakano et al., 2017 ). C. perfringens enterotoxins and beta-2 toxins ( β2 ), encoded by cpe and cpb2 genes, respectively, are considered to be significantly associated with human and animal intestinal diseases such as antibiotic-associated diarrhea, NE, and food poisoning ( Lindström et al., 2011 ; Hu et al., 2018 ; Zhang et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and multilocus sequence typing of Clostridium perfringens isolated from 4 duck farms in Shandong province, China

Liu

et al. 2020

Poultry Science

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Clostridium perfringens is an important zoonotic microorganism. The present study was undertaken to investigate prevalence, serotype distribution, antibiotic resistance, and genetic diversity of C. perfringens isolates from 4 duck farms in Shandong, China. In total, 424 samples of cloacal swabs and environment were collected from 3 commercial meat-type duck farms in Tai'an, Liaocheng, and Weifang and one breeder duck farm in Liaocheng between December 2018 and June 2019, of which, 207 (48.82%) samples were determined to be positive for C. perfringens ; a total of 402 isolates of C. perfringens were recovered, all of which were identified as type A; 30.85% of the isolates were positive for cpb2 gene; and cpe gene was found in 0.5% of the isolates. Antimicrobial susceptibility testing revealed that some of the isolates exhibited high antibiotic resistance, and 39.14% of the isolates were resistant to at least 5 classes of commonly used antibiotics. Multilocus sequence typing analysis showed that 85 representative isolates encompassed 54 different sequences types ( STs ), clustered in 5 clonal complexes ( CCs ) and 40 singletons. ST3, the most common ST in 54 STs, constituting 15.29% of all isolates, was also the most prevalent ST of isolates from the Liaocheng breeder duck farm (farm 3). CC1, the most prolific CC, containing 15.29% of the analyzed isolates, was the popular subtype of isolates from Liaocheng meat duck farm (farm 2). Although all the isolates belong to type A, the genetic diversity varied greatly in different regions; the Simpson's Diversity Index of STs for Liaocheng, Tai'an, and Weifang were 0.5941, 0.9198, and 0.9627, respectively. Some of cloacal isolates and environmental isolates were distributed in the same ST or CC, indicating close genetic relationship between cloacal isolates and environmental isolates. A portion of the strains from humans and ducks was found to be phylogenetically close. The close relationship between strains from humans and ducks, the high antibiotic resistance of C. perfringens , and the cpe -positive isolates indicated potential public health risks.

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Prevalence and multilocus sequence typing of Clostridium perfringens isolated from retail chicken products and diseased chickens in Tai'an region, China

Zhang

et al. 2021

Veterinary Medicine & Sci

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Background: Clostridium perfringens is an important zoonotic microorganism, which can cause diseases in animals and humans under suitable conditions. Contamination of C. perfringens in chicken products has been reported worldwide, but the genetic diversity and relationship of isolates were seldom analyzed. Objectives:The current study was undertaken to investigate the prevalence of C. perfringens from retail chicken products and sick chickens with suspected necrotic enteritis (NE) in Tai'an area, China. Methods:In total, 295 samples were collected from Tai'an large poultry retail market and veterinary hospital in 2018, then the isolates were tested for toxin genes, drug resistance and multilocus sequence typing (MLST).Results: Overall, 138 (46.78%) samples were determined to be positive for C. perfringens, and 99.37% of the isolates were identified as C. perfringens type A, with the remaining isolates being type F; 18.99% of the isolates were positive for cpb2 gene.Antimicrobial susceptibility testing revealed that 52.27% of the isolates from poultry retail market and diseased chickens showed multiple antibiotic resistance. MLST results showed that 50 analyzed isolates can be divided into 39 sequences types (STs), clustered in three clonal complexes (CCs) and 23 singletons. Although most of the isolates belong to type A, considerable genetic diversity can be observed, with the Simpson's diversity index up to 0.9181. MLST results and phylogenetic analysis showed that a portion of the isolates from humans and chickens were assigned to the same clusters in the phylogenetic tree or found to be in the same CCs, indicating the chicken isolates and the human isolates are related in certain stratification. Conclusions:This study showed that the contamination rate of C. perfringens in the local retail chicken products was relatively high. Most of the isolates exhibit broadspectrum antimicrobial resistance. The high antibiotic resistance of C. perfringens isolates and the relationship between isolates from human and chicken indicated potential public health risks.

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Multilocus sequence typing analyses of Clostridium perfringens type A strains harboring tpeL and netB genes

Cited by 29 publications

References 35 publications

Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province

Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province

Prevalence and multilocus sequence typing of Clostridium perfringens isolated from 4 duck farms in Shandong province, China

Prevalence and multilocus sequence typing of Clostridium perfringens isolated from retail chicken products and diseased chickens in Tai'an region, China

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