Multilocus sequence evaluation for differentiating species of the trematode Family Gastrothylacidae, with a note on the utility of mitochondrial COI motifs in species identification

Ghatani, Sudeep; Shylla, Jollin Andrea; Roy, Bishnupada; Tandon, Veena

doi:10.1016/j.gene.2014.07.046

Cited by 28 publications

(11 citation statements)

References 42 publications

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“…Untill now, the most common diagnostic method for F. elongates is the microscopical examination, but it’s time-consuming, and hard to distinguish with other paramphistomes. As a useful marker, mt genome has been widely used for species identification [ 6 – 10 ]. The complete mt genome of F. elongates can provide alternative molecular markers for the species identification, epidemiology and genetic diversity of paramphistomes.…”

Section: Resultsmentioning

confidence: 99%

Analysis of the complete Fischoederius elongatus (Paramphistomidae, Trematoda) mitochondrial genome

et al. 2015

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BackgroundFischoederius elongates is an important trematode of Paramphistomes in ruminants. Animals infected with F. elongates often don’t show obvious symptoms, so it is easy to be ignored. However it can cause severe economic losses to the breeding industry. Knowledge of the mitochondrial genome of F. elongates can be used for phylogenetic and epidemiological studies.FindingsThe complete mt genome sequence of F. elongates is 14,120 bp in length and contains 12 protein-coding genes, 22 tRNA genes, two rRNA genes and two non-coding regions (LNR and SNR). The gene arrangement of F. elongates is the same as other trematodes, such as Fasciola hepatica and Paramphistomum cervi. Phylogenetic analyses using concatenated amino acid sequences of the 12 protein-coding genes by Maximum-likelihood and Neighbor-joining analysis method showed that F. elongates was closely related to P. cervi.ConclusionThe complete mt genome sequence of F. elongates should provide information for phylogenetic and epidemiological studies for F. elongates and the family Paramphistomidae.

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Section: Resultsmentioning

confidence: 99%

Analysis of the complete Fischoederius elongatus (Paramphistomidae, Trematoda) mitochondrial genome

et al. 2015

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“…Previous studies have used the easily obtained ITS2 sequence as a molecular marker to distinguish among paramphistomoid species (Itagaki et al 2003 ; Rinaldi et al 2005 ; Goswami et al 2009 ; Lotfy et al 2010 ; Sanabria et al 2011 ; Ichikawa et al 2013 ; Shylla et al 2013 ; Ghatani et al 2014 ; Dube et al 2015 ). ITS2 is helpful for distinguishing paramphistomoid genera and differentiating more divergent species within a genus (Rinaldi et al 2005 ; Ghatani et al 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…ITS2 is helpful for distinguishing paramphistomoid genera and differentiating more divergent species within a genus (Rinaldi et al 2005 ; Ghatani et al 2012 ). Because mitochondrial DNA accumulates substitutions more frequently than the internal transcribed spacers, it is more useful to differentiate among closely related species, particularly cryptic species (Blouin, 2002 ; Vilas et al 2005 ; Locke et al 2015 ), or to reveal intraspecific variation (Ghatani et al 2014 ). Consequently, we used genetic distance values for cox 1 sequence data as the primary means to delineate species.…”

Section: Discussionmentioning

confidence: 99%

Loads of trematodes: discovering hidden diversity of paramphistomoids in Kenyan ruminants

et al. 2016

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SUMMARYParamphistomoids are ubiquitous and widespread digeneans that infect a diverse range of definitive hosts, being particularly speciose in ruminants. We collected adult worms from cattle, goats and sheep from slaughterhouses, and cercariae from freshwater snails from ten localities in Central and West Kenya. We sequenced cox1 (690 bp) and internal transcribed region 2 (ITS2) (385 bp) genes from a small piece of 79 different adult worms and stained and mounted the remaining worm bodies for comparisons with available descriptions. We also sequenced cox1 and ITS2 from 41 cercariae/rediae samples collected from four different genera of planorbid snails. Combining morphological observations, host use information, genetic distance values and phylogenetic methods, we delineated 16 distinct clades of paramphistomoids. For four of the 16 clades, sequences from adult worms and cercariae/rediae matched, providing an independent assessment for their life cycles. Much work is yet to be done to resolve fully the relationships among paramphistomoids, but some correspondence between sequence- and anatomically based classifications were noted. Paramphistomoids of domestic ruminants provide one of the most abundant sources of parasitic flatworm biomass, and because of the predilection of several species use Bulinus and Biomphalaria snail hosts, have interesting linkages with the biology of animal and human schistosomes to in Africa.

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“…Finalmente el ADN fue lavado con 300 μL de etanol 70% y centrifugado a 10.000 rpm por 5 minutos. of population genetic variability studies due to its universality and evolutionary importance (9). The mtDNA has characteristics such as its matrilineal inheritance, it is haploid, has a high rate of evolution and includes conserved and variable regions, which has made it a tool used in several studies (10)(11)(12).…”

Section: Extracción De Adnmentioning

confidence: 99%

“…Since 1970 the analysis of mitochondrial DNA (mtDNA) has been established as a powerful tool for evolutionary studies in animals. The cytochromo oxidase subunit I (COI) is part of a complex gene encoding of transmembrane proteins, involved in the electrical transport and catalysis of the respiratory chain of eukaryotic organisms and has been the subject respiratoria de organismos eucariotas y ha sido motivo de estudios poblacionales de variabilidad genética por su universalidad e importancia evolutiva (9). El ADNmt presenta características como su herencia matrilineal, es haploide, presenta una alta tasa de evolución y contiene regiones conservadas y variables, que lo han convertido en una herramienta utilizada en varios estudios (10)(11)(12).…”

Section: Introductionunclassified

Estimación de la diversidad genética de Anadara tuberculosa en cinco manglares de Tumaco, utilizando la enzima citocromo oxidasa I

2016

Rev MVZ Córdoba

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Objective. To estimate the genetic diversity of the Anadara tuberculosa en five mangrove swams of Tumaco, Nariño, Colombia using as a mitocondrial molecular marker the cytochromo oxidase sub-unit I (COI). Materials and methods. A total of 50 individuals were collected from the San Jorge, La Tiburonera, El Pajal, La Playa y Bajito Vaquería mangrove swamps, randomly selecting 10 specimens of each zone. The tissue sample was worked with absolute alcohol at ambient temperature in microtubes. DNA was extracted, and the mitocondrial DNA was amplified using the PCR technique (polymerase chain reaction). The amplified and quantified products of PCR were sequenced on both sides (Macrogen). Each one of the obtained sequences was edited and aligned. Later, the parameters of genetic diversity (haplotypical and nucleotidical) were measured, and the analysis of distribution between frequency pairs (Mistmach distribution) was elaborated. Finally, the analysis of nucleotidic variation and population structure (AMOVA) was completed. Results. The amplified product gene weighed 710 bp. The haplotypical diversity reported for all the populations was high (0.683±0.060) and the reported nucleotídical diversity was low for all the populations (0.040±0.020). The AMOVA results indicate that the variance amongst populations is low (4.20%) and that the variance within populations is high (95.80%). Conclusions. The studied populations are not structured and although there is a decrease of natural banks, the genetic diversity is high.

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Multilocus sequence evaluation for differentiating species of the trematode Family Gastrothylacidae, with a note on the utility of mitochondrial COI motifs in species identification

Cited by 28 publications

References 42 publications

Analysis of the complete Fischoederius elongatus (Paramphistomidae, Trematoda) mitochondrial genome

Analysis of the complete Fischoederius elongatus (Paramphistomidae, Trematoda) mitochondrial genome

Loads of trematodes: discovering hidden diversity of paramphistomoids in Kenyan ruminants

Estimación de la diversidad genética de Anadara tuberculosa en cinco manglares de Tumaco, utilizando la enzima citocromo oxidasa I

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