Multilocus sequence analysis provides basis for fast and reliable identification of Vibrio harveyi-related species and reveals previous misidentification of important marine pathogens

Cano-Gómez, Ana; Høj, Lone; Owens, Leigh; Andreakis, Nikolas

doi:10.1016/j.syapm.2011.09.001

Cited by 69 publications

(52 citation statements)

References 22 publications

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“…However, based on the fact that it was the only bacterial strain from the dead fish and previous reports indicated the pathogenicity of V. harveyi to fish, we assume that V. harveyi SNUVh-LW1 was the etiological factor of the mortality observed in the present study [1,3,5,10]. Thus, it is recommended to develop a proper treatment for this condition using the most effective antibiotics as V. harveyi SNUVh-LW1 was proved to be sensitive to all commercial antibiotics used in the present study except fluoroquinolones.…”

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confidence: 61%

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Isolation and identification of Vibrio harveyi from chub mackerel (Scomber japonicus)

Lee¹,

Jun²,

Giri³

et al. 2016

Korean Journal of Veterinary Research

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: For several days, there was a series of mortalities of chub mackerel (Scomber japonicus) that were reared for public exhibition in a private aquarium in Seoul, Korea. As part of the diagnosis of the dead fish, a bacterial isolate from the kidney was cultured, identified, and confirmed to be Vibrio (V.) harveyi using Vitek System 2 and 16S rRNA gene sequencing. Phylogenetic analysis was also performed by the neighbor-joining method. As a result, the V. harveyi isolated from chub mackerels of a private aquarium in Korea, called as SNUVh-LW1, was clustered in the same group with V. harveyi ATCC33843.

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confidence: 61%

“…Its fisheries have been performed mainly in the East Sea over several decades [7]. Vibrio (V.) harveyi belonging to the genus Vibrio has been reported to infect a wide range of marine organisms worldwide including fish and invertebrates [1,3,6]. The disease can cause mass mortalities in aquaculture species with significant economic impact.…”

mentioning

confidence: 99%

Isolation and identification of Vibrio harveyi from chub mackerel (Scomber japonicus)

Lee¹,

Jun²,

Giri³

et al. 2016

Korean Journal of Veterinary Research

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“…Once bacterial strains were found to be susceptible to VOB, the host bacteria were typed by multilocus sequence analysis (MLSA) using two loci, mreB and topA shown to work perfectly for the Harveyi clade [3].…”

Section: Strain Identificationmentioning

confidence: 99%

“…The Harveyi clade consists of eleven species: V. harveyi, V. campbellii, V. rotiferianus, V. alginolyticus, V. parahaemolyticus, V. mytili, V. natriegens, the newly described V. azureus [29], V. sagamiensis [30], V. owensii [2], V. communis [4] which is a junior synonym of V. owensii [3,8] and most recently, V. jasicida [31]. Many isolates of this clade particularly V. harveyi, V. campbellii and V. owensii, are bioluminescent and contribute to a very important penaeid hatchery disease called luminescent vibriosis.…”

Section: Introductionmentioning

confidence: 99%

Virulence Changes to Harveyi Clade Bacteria Infected with Bacteriophage from Vibrio owensii

Busico-Salcedo

Owens

2013

Indian J. Virol.

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Vibrio owensii is one of the most virulent vibrios known being able to kill crustacean larvae at 10 2 CFU ml -1 . This study describes virulence changes to naïve strains of Vibrio harveyi and Vibrio campbellii when infected with the bacteriophage VOB from a closely related species V. owensii 47666-1. The bacteriophage from V. owensii was induced into lytic phase by using mitomycin C at 100 ng ml -1 . One strain of V. harveyi and two strains of V. campbellii from 29 tested containing no prophage were susceptible to lysogenic conversion with VOB. Virulence changes induced in Harveyi clade bacteria included the upregulation of protein secretion, statistically significant increased haemolysin and chitinase production and increased mortality to nauplii of Penaeus monodon. No change in siderophore production was observed. Bacteriophage VOB is likely to be responsible for some of the virulence factors expressed by V. owensii. As this bacteriophage is able to infect strains of V. harveyi and V. campbellii this phage may contribute to increased virulence of other vibrios in aquaculture and in the natural environment.

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“…are widely distributed in coastal and estuarine waters, and inhabit eukaryotic marine hosts, including molluscs, shrimps and zooplankton, at high densities throughout the world (Thompson et al 2007). Recently, V. harveyi and related species have been recognized as some of the most significant causative agents of disease in marine-reared vertebrates and invertebrates, particularly shrimp and fish (Austin & Zhang 2006, Cano-Gomez et al 2011). Due to genetic and phenotypic similarities, discrimination among members of the Harveyi clade of vibrios remains difficult, and this has led to misidentifications and inaccurate estimations of a species' involvement in certain environments (Lin et al 2010, Hoffmann et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor

Jiang¹,

Shi²,

Ke³

et al. 2013

Dis. Aquat. Org.

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Mass mortality of farmed small abalone Haliotis diversicolor occurred in Fujian, China, from 2009 to 2011. Among isolates obtained from moribund abalones, the dominant species AP37 exhibited the strongest virulence. After immersion challenge with 10 6 CFU ml −1 of AP37, abalone mortalities of 0, 53 and 67% were induced at water temperatures of 20°C, 24°C, and 28°C, respectively. Following intramuscular injection, AP37 showed a low LD 50 (median lethal concentration) value of 2.9 × 10 2 CFU g −1 (colony forming units per gram abalone wet body weight). The LT 50 (median lethal time) values were 5.2 h for 1 × 10 6 CFU abalone . For further analysis of virulence, AP37 was screened for the production of extracellular factors. The results showed that various factors including presence of flagella and production of extracellular enzymes, such as lipase, phospholipase and haemolysin, could be responsible for pathogenesis. Based on its 16S rRNA gene sequence, strain AP37 showed > 98.8% similarity to Vibrio harveyi, V. campbellii, V. parahaemolyticus, V. algi nolyticus, V. na trie gens and V. rotiferianus, so it could not be identified by this method. However, multi-locus sequence analysis (MLSA) of concatenated sequences, including the rpoD, rctB, gyrB, toxR and pyrH genes, identified strain AP37 as V. harveyi. Phenotypic characters of AP37 were identified by API 20E. In antibiotic susceptibility tests, strain AP37 exhibited susceptibility to 7 antibiotics and resistance to 13. This is the first report of a V. harveyi-related species being linked with the mass mortality of adult abalone H. diversicolor in southern China.

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Multilocus sequence analysis provides basis for fast and reliable identification of Vibrio harveyi-related species and reveals previous misidentification of important marine pathogens

Cited by 69 publications

References 22 publications

Isolation and identification of Vibrio harveyi from chub mackerel (Scomber japonicus)

Isolation and identification of Vibrio harveyi from chub mackerel (Scomber japonicus)

Virulence Changes to Harveyi Clade Bacteria Infected with Bacteriophage from Vibrio owensii

Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor

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