Multilocus sequence analysis of Fusarium pseudograminearum reveals a single phylogenetic species

Scott, Jason B.; Chakraborty, Sukumar

doi:10.1016/j.mycres.2006.09.008

Cited by 38 publications

(30 citation statements)

References 41 publications

(52 reference statements)

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“…O'Donnell et al (2004) carried out phylogenetic analyses of 11 nuclear genes, including PHO, to resolve 9 phylogenetically distinct species within the F. graminearum clade. Scott and Chakraborty (2006) applied partial PHO gene sequences together with TEF1α, β-TUB and reductase (RED) sequences to demonstrate that F. pseudograminearum is a single phylogenetic species. It has been shown that PHO contained more variable and phylogenetically informative characters than the remaining loci and differentiated the F. pseudograminearum isolates into 3 groups.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, sequences of this gene have been used for the phylogenetic studies of trichothecene-producing species -F. graminearum (O'Donnell et al, 2000), F. pseudograminearum (Scott and Chakraborty, 2006), and F. culmorum (Obanor et al, 2010), and in the study describing F. sibiricum as a novel species (Yli-Mattila et al, 2011). We have demonstrated that PHO gene can be used as a target for speciesspecific detection of F. cerealis (Stakheev et al, 2013).…”

Section: Introductionmentioning

confidence: 93%

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Four-locus phylogeny of Fusarium avenaceum and related species and their species-specific identification based on partial phosphate permease gene sequences

Stakheev

Khairulina

Завриев

2016

International Journal of Food Microbiology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Four-locus phylogeny of Fusarium avenaceum and related species and their species-specific identification based on partial phosphate permease gene sequences

Stakheev

Khairulina

Завриев

2016

International Journal of Food Microbiology

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“…[13] indicated that F. avenaceum isolates from lisianthus are not phylogenetically distinct from those isolated from other hosts. Recent phylogenetic studies of other Fusarium species such as F. culmorum [17], F. poae [18,19] and F. pseudograminearum [20] showed that intraspecific groups within these morphospecies are not clearly associated with a particular geographic area. Similarly, no such association was found in F. avenaceum [13].…”

Section: Discussionmentioning

confidence: 99%

“…The aim of this study was to: (i) determine the phylogenetic relationships among 63 F. avenaceum isolates with respect to host (wheat) and geographic origin; (ii) determine whether distinct evolutionary lineages are present within F. avenaceum ; (iii) determine recombination events within F. avenaceum . MLS (multilocus sequence analysis) used in this study incorporated data sets of EF1α , IGS rDNA and RPB2 (RNA polymerase II) that are widely used in fungal phylogenetics [13,16–20]. We also incorporated a partial sequence of ESYN1 (enniatin synthase) involved in enniatin synthesis by F. avenaceum and a partial sequence of NAT2 (arylamine N-acetyltransferase) [16] which belongs to the NAT gene family, encoding xenobiotic metabolizing enzymes in various prokaryotes and eukaryotes.…”

Section: Introductionmentioning

confidence: 99%

Multilocus Phylogenetics Show High Intraspecific Variability within Fusarium avenaceum

Kulik

Pszczółkowska

Łojko

2011

IJMS

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Fusarium avenaceum is a common soil saprophyte and plant pathogen of a variety of hosts worldwide. This pathogen is often involved in the crown rot and head blight of cereals that affects grain yield and quality. F. avenaceum contaminates grain with enniatins more than any species, and they are often detected at the highest prevalence among fusarial toxins in certain geographic areas. We studied intraspecific variability of F. avenaceum based on partial sequences of elongation factor-1 alpha, enniatin synthase, intergenic spacer of rDNA, arylamine N-acetyltransferase and RNA polymerase II data sets. The phylogenetic analyses incorporated a collection of 63 F. avenaceum isolates of various origin among which 41 were associated with wheat. Analyses of the multilocus sequence (MLS) data indicated a high level of genetic variation within the isolates studied with no significant linkage disequilibrium. Correspondingly, maximum parsimony analyses of both MLS and individual data sets showed lack of clear phylogenetic structure within F. avenaceum in relation to host (wheat) and geographic origin. Lack of host specialization indicates no host selective pressure in driving F. avenaceum evolution, while no geographic lineage structure indicates widespread distribution of genotypes that resulted in nullifying the effects of geographic isolation on the evolution of this species. Moreover, significant incongruence between all individual tree topologies and little clonality is consistent with frequent recombination within F. avenaceum.

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“…An example is phosphate permease gene (PHO), which was used for the taxonomic studies of several groups of Fusarium pathogens. Thus, J.B. Scott and S. Chakraborty [40] have successfully used it as a marker to classify F. pseudograminearum as a separate species. Later PHO gene was used in four-locus phylogeny analysis [33] which allows to distinguish a number of isolates from Siberia and the Far East, originally identified as F. poae, as a separate species F. sibiricum.…”

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confidence: 99%

MOLECULAR GENETIC APPROACHES FOR INVESTIGATION OF TAXONOMY AND SPECIFIC IDENTIFICATION OF TOXIN-PRODUCING Fusarium SPECIES: ACHIEVEMENTS AND PROBLEMS (review)

Stakheev¹,

Samokhvalova²,

Ryazantsev³

et al. 2016

S-h. biol.

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A b s t r a c tAscomycetous fungi of the genus Fusarium, which was separated as a single taxonomic group in 1809 by Heinrich Friedrich Link, are distributed worldwide and have different ecological niches. They have been found in soil and plants as endophytes, saprotrophs and parasites. Infections of agricultural plants, caused by Fusarium fungi, lead to annual damage, estimated in hundred million dollars. In addition to decrease harvest quality and quantity, Fusarium fungi are able to produce toxic metabolites (fusariotoxins). The consumption of fusariotoxin-contaminated winter grain led to deaths of tens of thousands of people in Volga and Ural regions in 1930s-1940s. These factors make necessary investigation of morphology, biochemistry and genetics of those organisms. Nowadays there is no universal taxonomic system of the genus Fusarium, so it is not possible to identify an isolate to species level in many cases. The high variability of morphological structures, and, on the other side, their similarity for closely related species, are major problems for researchers which use classical methods for identification and systematization of this group of fungi. Today, the molecular methods, based on the use of specific DNA sequence, are playing an increasingly important role in Fusarium systematics. The application of this approach has led to establishment of a number of new species in the genus. T. Yli-Mattila et al. (2009, 2011 used multilocus phylogenetic analysis to describe the novel species F. sibiricum and F. ussurianum which were found in Siberia and Far East and proved to be morphologically similar to F. graminearum and F. langsethiae, respectively. The authors of this paper identified the species F. torulosum (morphologically similar to F. avenaceum) in Russia for the first time based on the use of DNA markers. It is no less important that the analysis of interand intraspecific polymorphism makes possible the development of highly-specific assays for molecular detection of Fusarium fungi, including ones which infect plants and produce mycotoxins. The use of these assays (for example, ones described by M. Nicolaisen et al., 2009 and A.A. Stakheev et al., 2011) allows not only to detect the contamination of plants by mycotoxin producers, but also to estimate the quantity of their content in a probe. At the same time, a lot of questions are still unanswered: particularly, simultaneous application of different species concepts (morphological, biological and phylogenetic) makes it difficult to develop universal taxonomic system of the genus; also the most informative and reliable marker, which would reflect the evolutionary history of the genus, has not been found. The main achievements and problems of Fusarium phylogenetics, and perspectives of the application of molecular approach for studies of this group of organisms are discussed in this review.

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Multilocus sequence analysis of Fusarium pseudograminearum reveals a single phylogenetic species

Cited by 38 publications

References 41 publications

Four-locus phylogeny of Fusarium avenaceum and related species and their species-specific identification based on partial phosphate permease gene sequences

Four-locus phylogeny of Fusarium avenaceum and related species and their species-specific identification based on partial phosphate permease gene sequences

Multilocus Phylogenetics Show High Intraspecific Variability within Fusarium avenaceum

MOLECULAR GENETIC APPROACHES FOR INVESTIGATION OF TAXONOMY AND SPECIFIC IDENTIFICATION OF TOXIN-PRODUCING Fusarium SPECIES: ACHIEVEMENTS AND PROBLEMS (review)

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