Multilocus phylogeny of Clonostachys subgenus Bionectria from Brazil and description of Clonostachys chloroleuca sp. nov.

Moreira, Guilherme Musse; Abreu, Lucas M.; Carvalho, Vívian G.; Schroers, Hans-Josef; Pfenning, Ludwig H.

doi:10.1007/s11557-016-1224-6

Cited by 31 publications

(45 citation statements)

References 23 publications

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“…Our results were in agreement with a recent genomic study identifying the biosynthetic gene clusters for verticillins in C. rogersoniana [76]. As the ITS region alone is not informative to identify species in certain orders of the Ascomycota, including Hypocreales [33], future taxonomic and molecular phylogenetic studies will incorporate sequence data from protein-coding regions to more precisely identify species names for verticillin-producing strains [54]. The sequence data were deposited in GenBank (accession numbers: KX845687, KX845688, MH421853, MH421854, MH421855, MH421856, MH421857, MH421858, MH421859, and MH421860).…”

Section: Methodssupporting

confidence: 89%

Media studies to enhance the production of verticillins facilitated by in situ chemical analysis

Amrine

Raja

Darveaux

et al. 2018

Journal of Industrial Microbiology and Biotechnology

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Verticillins are a group of epipolythiodioxopiperazine alkaloids that have displayed potent cytotoxicity. To evaluate their potential further, a larger supply of these compounds was needed for both in vivo studies and analogue development via semisynthesis. To optimize the biosynthesis of these secondary metabolites, their production was analyzed in two different fungal strains (MSX59553 and MSX79542) under a suite of fermentation conditions. These studies were facilitated by the use of the droplet-liquid microjunction-surface sampling probe (droplet probe), which enables chemical analysis in situ directly from the surface of the cultures. These experiments showed that the production of verticillins was greatly affected by growth conditions; a significantly higher quantity of these alkaloids was noted when the fungal strains were grown on an oatmeal-based medium. Using these technologies to select the best among the tested growth conditions, the production of the verticillin analogues was increased while concomitantly decreasing the time required for fermentations from 5 weeks to about 11 days. Importantly, where we could previously supply 5–10 mg every 6 weeks, we are now able to supply 50–150 mg quantities of key analogues per month via laboratory scale fermentation.

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Section: Methodssupporting

confidence: 89%

Media studies to enhance the production of verticillins facilitated by in situ chemical analysis

Amrine

Raja

Darveaux

et al. 2018

Journal of Industrial Microbiology and Biotechnology

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“…MFS transporter genes induced during interactions with B. cinerea and F. graminearum almost exclusively belonged to these expanded MFS families, indicating that efflux-mediated protection against exogenous or endogenous secondary metabolites and nutrient uptake are important components of the mycoparasitic attack in C. rosea. MFS transporters were reported to be induced in several other mycoparasitic species, including the closely related C. chloroleuca (Moreira, Abreu, Carvalho, Schroers, & Pfenning, 2016;Sun, Sun, & Li, 2015), Trichoderma spp. (Atanasova, Le Crom et al, 2013;Seidl et al, 2009;Steindorff et al, 2014), Tolypocladium ophioglossoides (Quandt, Di, Elser, Jaiswal, & Spatafora, 2016), Escovopsis weberi (de Man et al, 2016), Pythium oligandrum (Horner, Grenville-Briggs, & Van West, 2012) and Ampelomyces quisqualis (Siozios et al, 2015), but without proper identification of family classification it is difficult to speculate about their exact biological role in these species.…”

Section: Discussionmentioning

confidence: 99%

The mycoparasitic fungus Clonostachys rosea responds with both common and specific gene expression during interspecific interactions with fungal prey

Nygren

Dubey

Zapparata

et al. 2018

Evolutionary Applications

103

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Clonostachys rosea is a necrotrophic mycoparasitic fungus, used for biological control of plant pathogenic fungi. A better understanding of the underlying mechanisms resulting in successful biocontrol is important for knowledge‐based improvements of the application and use of biocontrol in agricultural production systems. Transcriptomic analyses revealed that C. rosea responded with both common and specific gene expression during interactions with the fungal prey species Botrytis cinerea and Fusarium graminearum. Genes predicted to encode proteins involved in membrane transport, biosynthesis of secondary metabolites and carbohydrate‐active enzymes were induced during the mycoparasitic attack. Predicted major facilitator superfamily (MFS) transporters constituted 54% of the induced genes, and detailed phylogenetic and evolutionary analyses showed that a majority of these genes belonged to MFS gene families evolving under selection for increased paralog numbers, with predicted functions in drug resistance and transport of carbohydrates and small organic compounds. Sequence analysis of MFS transporters from family 2.A.1.3.65 identified rapidly evolving loop regions forming the entry to the transport tunnel, indicating changes in substrate specificity as a target for selection. Deletion of the MFS transporter gene mfs464 resulted in mutants with increased growth inhibitory activity against F. graminearum, providing evidence for a function in interspecific fungal interactions. In summary, we show that the mycoparasite C. rosea can distinguish between fungal prey species and modulate its transcriptomic responses accordingly. Gene expression data emphasize the importance of secondary metabolites in mycoparasitic interactions.

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“…The genus Clonostachys (Bionectriaceae, Hypocreales) includes species that are commonly found in soils, as well as in plants as endophytes, epiphytes or saprotrophs. Species of this genus have also been reported as mycoparasites and less frequently as parasites of invertebrates containing chitin, including some nematodes and insects [1–4]. The sexual morphs of species of the genus Clonostachys were placed in the genus Bionectria which was further distinguished into six subgenera Astromata , Bionectria , Epiphloea , Myronectria , Uniparietina and Zebrinella [1].…”

Section: Introductionmentioning

confidence: 99%

“…penicillate and verticillate or only monomorphic penicillium-like conidiophores. The secondary conidiophores generally form slimy masses of conidia, which occur in various colours including white, pale yellowish to salmon, orange or greenish [1, 4, 6].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Clonostachys viticola sp. nov., a novel species isolated from Vitis vinifera

Torcato

Gonçalves

Rodríguez-Gálvez

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A collection of fungal isolates obtained from crop plants, specifically grapevine and blueberry, in Peru were characterised through morphological and DNA sequence analyses of the nuclear ribosomal internal transcribed spacer (ITS), beta-tubulin (tub2) and translation elongation factor 1-alpha (tef-1α) regions. Isolates produced monomorphic and dimorphic conidiophores typical of members of the genus Clonostachys. Single-and multi-locus gene phylogenies confirmed the isolates as representing members of the genus Clonostachys, more closely related to species in the subgenus Bionectria. In phylogenetic analyses the isolates grouped in two separate clades, one corresponding to the species Clonostachys pseudochroleuca and the other one distinct from all known species of the genus Clonostachys. These isolates are recognized as representing a novel species species for which the name Clonostachys viticola is proposed. Clonostachys chloroleuca CBS 141588 Clonostachys chloroleuca CBS 141592 Clonostachys chloroleuca CBS 141589 Clonostachys rhizophaga CBS 361.77 Clonostachys rosea f. cantenulata CBS 154.27 Clonostachys rosea f. rosea CBS 710.86 Clonostachys wenpingii HMAS 172156 Clonostachys byssicola CBS 364.78 Clonostachys eriocamporesiana MFLUCC 17-2620 Clonostachys pseudochroleuca CBS 187.94 Clonostachys pseudochroleuca CML 1940 Clonostachys pseudochroleuca CML 2406 Clonostachys pseudochroleuca CAA 948 Clonostachys pseudochroleuca MUM 18.50/CAA 947 Clonostachys pseudochroleuca CML 1982 Clonostachys pseudochroleuca CBS 192.94 Clonostachys pseudochroleuca CML 1983 Clonostachys viticola CAA 946 Clonostachys viticola CAA 945 Clonostachys viticola MUM 18.51/CAA 944 Clonostachys rogersoniana CML 1923 Clonostachys rogersoniana CML 1926 Clonostachys buxi CBS 696.93 Clonostachys candelabrum CBS 504.67 Calonectria ilicicola CBS 190.50

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Multilocus phylogeny of Clonostachys subgenus Bionectria from Brazil and description of Clonostachys chloroleuca sp. nov.

Cited by 31 publications

References 23 publications

Media studies to enhance the production of verticillins facilitated by in situ chemical analysis

Media studies to enhance the production of verticillins facilitated by in situ chemical analysis

The mycoparasitic fungus Clonostachys rosea responds with both common and specific gene expression during interspecific interactions with fungal prey

Clonostachys viticola sp. nov., a novel species isolated from Vitis vinifera

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