Multilocus Enzyme Analysis of the Genus Aeromonas and its Use for Species Identification

Altwegg, Martin; Reeves, Michael W.; Altwegg-Bissig, Regula; Brenner, Don J.

doi:10.1016/s0934-8840(11)80765-6

Cited by 35 publications

(14 citation statements)

References 15 publications

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“…We used multilocus enzyme electrophoresis (MEE) which was adapted by Selander and his team to prokaryotes, and which has already given interesting contributions in population genetics and epidemiology [16]. Moreover, the MEE shows a good correlation with different methods of DNA hybridization [17][18][19]. Moreover, the MEE shows a good correlation with different methods of DNA hybridization [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Multilocus genetic relationships between clinical and environmental Aeromonas strains

Tonolla

1991

FEMS Microbiology Letters

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Allelic variations in the chromosomal genome of 120 isolates of motile Aeromonas (A. hydrophila, A. sobria and A. caviae) and eight reference strains with one Plesiomonas shigelloides were assessed by analysis of electrophoretically demonstrable polymorphism in 16 genes encoding metabolic enzymes. The strains were collected from humans (n = 59) and from the aquatic environment (n = 61) in canton Tessin, Switzerland. Clustering of the electrophoretic types (ET) from a matrix of pairwise genetic distances, based on the 16 enzyme loci, confirmed the genetic distinctness of the three species. Furthermore, A. hydrophila and A. sobria were divided in three and two groups respectively. For each species clinical strains were well differentiated from those collected in the environment.

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Section: Introductionmentioning

confidence: 99%

Multilocus genetic relationships between clinical and environmental Aeromonas strains

Tonolla

1991

FEMS Microbiology Letters

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“…sobria and Aer. caviae ), and by Altwegg et al . (1991) ( H = 0.65) in a MLEE analyses with 153 Aeromonas strains at 11 enzyme loci.…”

Section: Discussionmentioning

confidence: 84%

Genetic diversity and population structure of Aeromonas hydrophila, Aer. bestiarum, Aer. salmonicida and Aer. popoffii by multilocus enzyme electrophoresis (MLEE)

Miñana‐Galbis

Farfán

Fusté

et al. 2004

Environmental Microbiology

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Genetic diversity, genetic relationship, identification and population structure of 120 Aeromonas strains (including Aer. hydrophila, Aer. bestiarum, Aer. salmonicida and Aer. popoffii) isolated from various sources were studied by analysis of 15 genetic loci by multilocus enzyme electrophoresis (MLEE). All 15 loci were polymorphic, with an average of 9.4 alleles per locus and a mean genetic diversity (H) of 0.64. Cluster analysis defined at H < or = 0.7 differentiated most of the taxa analysed except the Aer. popoffii and Aer. bestiarum strains, which showed a close genetic relationship. Allelic frequencies of five loci (EST1, HEX, IDH, LDH1 and MDH) identified 94% of the strains. The index of association (IA) for the total sample was 2.38 and IA values calculated for the different populations were always significantly different from zero. These results suggest that the population structure of this Aeromonas sample is strongly clonal, confirm the taxonomic status of the analysed species in population genetics terms, and show the usefulness of MLEE for identifying Aeromonas species.

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“…In the literature [1,3], the values of genetic diversity among enzymatic loci (HS) of Aeromonas spp. vary extensively between 0.09 and 0.88.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of anthropogenic pollution in river water based on the genetic diversity of Aeromonas hydrophila

Korzekwa¹,

Gołaś²,

Harnisz³

2012

Archives of Environmental Protection

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Aeromonas hydrophila is a valuable indicator of the quality of water polluted by sewage and pathogens that pose a risk for humans and cold-blooded animals, including fi sh. The main aim of this research was to evaluate anthropogenic pollution of river water based on genetic diversity of 82 A. hydrophila strains by means of RAPD, semi-random AP-PCR (ISJ) and the rep-BOX conservative repeats test. Genetic diversity of A. hydrophila was HT = 0.28 (SD = 0.02) for all DNA markers (RAPD, semi random and rep-BOX).None of the analyzed electrophoretic patterns was identical, implying that there were many sources of strain transmission. The presence of genes for aerolysin (aerA), hemolysin (ahh1) and the cytotoxic enzyme complex (AHCYTOGEN) was verifi ed for all tested strains, and drug resistance patterns for tetracycline, enrofl oxacin and erythromycin were determined. The most diverse A. hydrophila strains isolated from river water were susceptible to enrofl oxacine (HS = 0.27), whereas less diverse strains were susceptible to erythromycin (HS = 0.24). The presence of the multidrug resistance marker (ISJ4-25; 1100 bp locus) in the examined strains (resistant to three analyzed drugs) indicates that intensive fi sh cultivation affects the microbiological quality of river water.

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Multilocus Enzyme Analysis of the Genus Aeromonas and its Use for Species Identification

Cited by 35 publications

References 15 publications

Multilocus genetic relationships between clinical and environmental Aeromonas strains

Multilocus genetic relationships between clinical and environmental Aeromonas strains

Genetic diversity and population structure of Aeromonas hydrophila, Aer. bestiarum, Aer. salmonicida and Aer. popoffii by multilocus enzyme electrophoresis (MLEE)

Evaluation of anthropogenic pollution in river water based on the genetic diversity of Aeromonas hydrophila

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