Multilocus DNA Fingerprinting Using Nonradioactively Labeled Oligonucleotide Probes Specific for Simple Repeat Elements

Yearling male great tits, Parus major, suffer more strongly from cuckoldry than older males

“…All details on DNA fingerprinting procedures have been described previously (Epplen, 1992;Lubjuhn, 1994), hence the methods are only briefly summarised here. The blood samples were diluted in 250 ml APS buffer (Arctander, 1988) and stored at À20 1C until analysed.…”

Telomeric lengths and telomerase activity in liposarcomas

“…Ten micrograms of DNA was digested with HinfI, and the DNA fragments were separated by electrophoresis on 0.8% agarose gels. The gels were dried under vacuum, and in-gel hybridization was performed according to the protocol of Epplen [18] by using the radioactively labeled probe (TTAGGG) 3 . Telomere signals were measured by estimating the band size at the point with greatest optical density.…”

Potential of (GATA)n microsatellites from rice for inter- and intra-specific variability studies

“…(GATA) n sequences which have not been mapped so far in rice, are the most frequent among tetranucleotide repeats with 270 poly (GATA) n motifs in the entire rice genome [42]. The degree of polymorphism shown by microsatellites rather than their abundance in the genome contributes to their usefulness in genome analysis For fingerprinting plant genomes, Epplen [51] has shown that oligonucleotides based on -GATA- or -GACA-microsatellite motifs are amongst the most frequently used for fingerprinting plant genomes. We, therefore, thought that it would be interesting to isolate these (GATA) n -containing regions from rice and convert them into PCR amplifiable microsatellites, to determine variations at these highly polymorphic loci.…”