Multilineage potential and proteomic profiling of human dental stem cells derived from a single donor

How has dental pulp stem cells isolation been conducted? A scoping review

“…association between the enzymatic technique and the use of mechanical devices to intensify cell dissociation has also been used. 21 Our findings revealed that 56.3% of the included studies applied the enzymatic technique, and 33.5% associated this methodology with the mechanical cell dissociation step. The enzymatic technique consists of removing DPSCs from dental pulp by using enzymes, such as collagenase, dispase 19 and trypsin, 22 to dissociate cells from each other and from its extracellular matrix, and obtain a heterogeneous singularized cell suspension.…”

Tooth-derived stem cells: Update and perspectives

“…DPSCs also failed to form lipid-laden adipocytes in vitro, whereas BMSCs are capable of differentiating into adipocytes [44] . However, more recent studies demonstrate that DPSCs can differentiate into adipocyte cells when other supplements are added to the adipogenic induction medium [46,65] . When transplanted in vivo, some DPSC clones differentiate into aligned odontoblast-like cells, with prolonged processes oriented into newly formed dentin-like structures [44,46] , whereas BMSCs form distinct lamellae of bone [44] .…”

“…heterogeneity of DPFCs was analyzed, it was observed that, although all cloned cell lines were positive for MSCrelated surface markers (CD105, CD44, CD29) and negative for hematopoietic markers (CD34, CD117), they were different in terms of proliferation and mineralization patterns, indicating that they could be committed to distinct lineages [72] . In order to avoid donor variability, TDSCs from follicle, pulp and papilla were isolated from a single donor tooth and the morphology, proliferation rate, expression of MSC-specific and pluripotency markers, and in vitro differentiation into osteoblasts, adipocytes, chondrocytes and hepatocyte-like cells were compared [65] . Adherent, fibroblast-like morphology was observed in all TDSCs cultured under the same standard conditions, and DFPCs were more proliferative than DPSCs and SCAP [65] .…”

“…In this context, dental tissues have also been investigated as niches of MSCs, and many toothderived SCs (TDSCs) have been identified and characterized, including dental pulp SCs (DPSCs) [44][45][46][47][48] , SCs from human exfoliated deciduous teeth (SHED) [49][50][51][52][53] , periodontal ligament SCs (PDLSCs), dental follicle progenitor cells (DFPCs) [54][55][56] , SCs from apical papilla (SCAP) [19,[56][57][58][59] , and periodontal ligament of deciduous teeth SCs (DePDL) [50,51,[60][61][62] (Figure 1). Dental tissues are an accessible source of MSCs that can be obtained with limited morbidity and without additional risks to the donor, as extracted/exfoliated teeth represent a waste product of dental procedures [13,[63][64][65] . However, the properties of these TDSCs and their feasibility for regenerating tissues still need to be investigated in greater detail.…”

Fractal and gray level co-occurrence matrix texture analysis of senescent and non-senescent deciduous teeth stem cells: A pilot study

“…The main limitation is the number of biological specimens from which images of DTSCs nuclei were extracted. Although, stem cells were obtained from only one tooth, this is not uncommon in stem cell research, where different experimental procedures are often done on stem cells isolated from one tooth or patient [43][44][45]. Thus, the next research step could be the application of these two methods on a larger number of specimens and on different types of mesenchymal stem cells isolated from various tissues.…”