Multilineage differentiation of dental follicle cells and the roles of Runx2 over‐expression in enhancing osteoblast/cementoblast‐related gene expression in dental follicle cells

Pan, Keqing; Qi-zhong, Sun; Zhang, J.; Ge, Shaohua; Li, Shuang; Zhao, Yijiao; Yang, Pishan

doi:10.1111/j.1365-2184.2010.00670.x

Cited by 52 publications

(51 citation statements)

References 52 publications

(62 reference statements)

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“…Vascular smooth muscle cell (VSMC) can lose the expression of smooth muscle lineage markers and begin to express osteogenic markers and deposit a mineralized bone-like matrix [14][15][16][17][18][19]. During the mineralization of VSMC, runt-related transcription factor2 (Runx2), a transcription factor expressed at early stages of osteoblast differentiation, is upregulation [20], and in concert with osterix by regulating the expression of osteoblast-related gene [21], such as osteopontin (OPN) contributes to VC [22]. Recent study suggested that klotho knockdown can accelerate VSMC calcification through a Runx2-dependent pathway.…”

Section: Introductionmentioning

confidence: 99%

Thyroid Hormone Attenuates Vascular Calcification Induced by Vitamin D3 Plus Nicotine in Rats

et al. 2014

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Thyroid hormones (THs) including thyroxine (T4) and triiodothyronine (T3) play critical roles in bone remodeling. However, the role and mechanism of THs in vascular calcification (VC) have been unclear. To explore the pathophysiological roles of T3 on VC, we investigated the changes in plasma and aortas of THs concentrations and the effect of T3 on rat VC induced by vitamin D3 plus nicotine (VDN). VDN-treated rat showed decreased plasma T3 content, increased vascular calcium deposition, and alkaline phosphatase (ALP) activity. Administration of T3 (0.2 mg/kg body weight IP) for 10 days greatly reduced vascular calcium deposition and ALP activity in calcified rat aortas when compared with controls. Concurrently, the loss of smooth muscle lineage markers α-actin and SM22a was restored, and the increased bone-associated molecules, such as runt-related transcription factor2 (Runx2), Osterix, and osteopontin (OPN) levels in calcified aorta, were reduced by administration of T3. The suppression of klotho in calcified rat aorta was restored by T3. Methimazole (400 mg/L) blocked the beneficial effect of T3 on VC. These results suggested that T3 can inhibit VC development.

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Section: Introductionmentioning

confidence: 99%

Thyroid Hormone Attenuates Vascular Calcification Induced by Vitamin D3 Plus Nicotine in Rats

et al. 2014

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“…Runx2 (Cbfa1) is a key transcription factor essential for skeletal and tooth development, acting at very early stages to drive the expression of mineralization proteins, like DSPP, BSP, ALP, OCN etc. [42,43]. Remarkably, low expression of Runx2 has been previously observed in differentiating odontoblasts, unlike the high expression in other mineralizing tissues; suggesting the odontoblastic lineage diverges from the osteoblastic [44].…”

Section: Xxxe15mentioning

confidence: 86%

Human treated dentin matrices combined with Zn-doped, Mg-based bioceramic scaffolds and human dental pulp stem cells towards targeted dentin regeneration

et al. 2016

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“…We noticed that the terms, DFSCs (dental follicle stem cells) and DFC/DFCs (dental follicle cells) have been used in the literature to denote the DF derived cells that are capable of differentiation. For example, in the publications by Pan et al and Guo et al [21, 22], the DFCs were established by culturing the DF derived cells in α-MEM +FBS, which was the same medium used in our study to establish the DFSCs. We use DFSCs to reflect the facts that the cell population possesses the stem cell properties of multi-lineage differentiation.…”

Section: Discussionmentioning

confidence: 99%

The role of dentin matrix protein 1 (DMP1) in regulation of osteogenic differentiation of rat dental follicle stem cells (DFSCs)

Rad¹,

Liu²,

He³

et al. 2015

Archives of Oral Biology

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Objectives Primary isolated dental follicle stem cells (DFSCs) possess a strong osteogenesis capability, and such capability is reduced during in vitro culture. Because dentin matrix protein 1 (DMP1) is essential in the maturation of osteoblasts, our objectives were to determine (1) the expression of DMP1 in the DFSCs, (2) the correlation between DMP1 expression and osteogenic capability of DFSCs, and (3) the ability of DMP1 to promote osteogenic differentiation of DFSCs. Methods DFSCs and their non-stem cell counterpart dental follicle cells (DFC) were established from postnatal rat pups. Expression of DMP1 in the DFSCs and DFC was determined using real-time RT-PCR and western blotting. Different passages of DFSCs were subjected to osteogenic induction. The correlation between osteogenesis and DMP1 expression was analyzed. Then, expression of DMP1 in the DFSCs was knocked-down using siRNA, followed by osteogenic induction to evaluate the effect of DMP1-knockdown. Finally, the late passage DFSCs with reduced DMP1 expression and osteogenic capability were cultured in osteogenic induction medium containing mouse recombinant DMP1 (mrDMP1) to determine if DMP1 can restore osteogenesis of DFSCs. Results DFSCs expressed much higher levels of DMP1 than did DFC. DMP1 expression was correlated with the osteogenic capability of DFSCs. Knockdown of DMP1 expression markedly decreased the osteogenesis and osteogenic gene expression in the DFSCs whereas adding mrDMP1 protein to the osteogenic induction medium enhanced osteogenesis. Conclusions DMP1 is highly expressed in the DFSCs, but minimally expressed in non-stem cell DFC. DMP1 appears to play an important role for osteogenic differentiation of the DFSCs.

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Multilineage differentiation of dental follicle cells and the roles of Runx2 over‐expression in enhancing osteoblast/cementoblast‐related gene expression in dental follicle cells

Cited by 52 publications

References 52 publications

Thyroid Hormone Attenuates Vascular Calcification Induced by Vitamin D3 Plus Nicotine in Rats

Thyroid Hormone Attenuates Vascular Calcification Induced by Vitamin D3 Plus Nicotine in Rats

Human treated dentin matrices combined with Zn-doped, Mg-based bioceramic scaffolds and human dental pulp stem cells towards targeted dentin regeneration

The role of dentin matrix protein 1 (DMP1) in regulation of osteogenic differentiation of rat dental follicle stem cells (DFSCs)

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