Multilevel assessment of ivermectin effects using different zebrafish life stages

Oliveira, Rhaul; Grisólia, César Koppe; Monteiro, Marta S.; Soares, Amadeu M.V.M.; Domingues, Inês

doi:10.1016/j.cbpc.2016.04.004

Cited by 46 publications

(29 citation statements)

References 52 publications

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“…Behavioral patterns were shown to be a robust, sensitive and reliable tool that can be used as early indicator of stress, and thus were used also to understand the effect at population and ecosystem level [29,30]. Behavioral endpoints were also successfully applied for the identification of toxicity of several compound classes with neurotoxic MoA, including pesticides mixtures, pharmaceuticals and personal care products (PPCPs), metals and classical POPs [63][64][65][66]. However, it may be expected that neurotoxic effects are not restricted to these chemicals, but may be exhibited by many more.…”

Section: Discussionmentioning

confidence: 99%

Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos

et al. 2019

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Background: The toxicological characterization of sediments is an essential task to monitor the quality of aquatic environments. Many hazardous pollutants may accumulate in sediments and pose a risk to the aquatic community. The present study provides an attempt to integrate a diagnostic whole mixture assessment workflow based on a slightly modified Danio rerio embryo acute toxicity test with chemical characterization. Danio rerio embryos were directly exposed to sieved sediment (≤ 63 μm) for 96 h. Sediment samples were collected from three polluted sites (Kramfors, Sundsvall and Örnsköldsvik) in the Gulf of Bothnia (Sweden) which are characterized by a long history of pulp and paper industry impact. Effect data were supported by chemical analyses of 237 organic pollutants and 30 trace elements. Results: The results show that malformations and neurotoxic compounds are the main drivers of differentiation in chemical and effects analyses, respectively. Specific spinal cord malformations and delayed hatching were observed only in sediments from Kramfors while light hyperactivity was seen only after exposure to sediments from Sundsvall. Conclusions: Our experiments demonstrate that specific chemical profiles lead to specific effect patterns in Danio rerio embryos. In fact, behavioral endpoints could help detect the exposure to neurotoxins, and the observation of body malformations seems to be a potential tool for the identification of site-specific pollutants as polychlorinated biphenyl (PCBs), brominated flame retardants (BFRs) and several pesticides. Overall, results show the suitability of Danio rerio embryos for the fast screening of sediment samples.

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Section: Discussionmentioning

confidence: 99%

Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos

et al. 2019

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“…For abamectin, however, the neurotoxic mode of action and the consequently reduced embryo movement is likely to have played the greatest role on embryo hatching success. The toxicity values for ivermectin indeed indicate that effects on embryo movements may ensue at low concentrations (Weil et al, 2009;Carlsson et al, 2013;Oliveira et al, 2016, Table 1). Difenoconazole, in turn, may affect the hatching gland by inducing yolk sac abnormalities, which may further disrupt the synthesis of the hatching enzyme chorionase, and subsequently lead to a decrease of the hatching rate (Mu et al, 2016).…”

Section: Toxicity Of Embryos To Individual Compoundsmentioning

confidence: 88%

“…De fato, a partir de dados de mortalidade encontrados em diferentes estudos realizados com adultos e embriões de Danio rerio (Tabela 2), pode-se concluir que o estágio de vida mais sensível do zebrafish é a fase adulta (DOMINGUES et al, 2016;OLIVEIRA et al, 2016;SANCHES et al, 2017;TIŠLER;KOŽUH ERŽEN, 2006;WEIL et al, 2009 . No Brasil, este praguicida é muito utilizado principalmente nas culturas de morango e batata, e alguns estudos mostram os efeitos tóxicos causados por esse composto e suas formulações comerciais a diferentes organismos (DONG et al, 2013;MU et al, 2015MU et al, , 2016PADILLA et al, 2012;SANCHES et al, 2017).…”

Section: Discussionunclassified

“…difenoconazol) vem sendo amplamente utilizados nas culturas de morango no Brasil (SILVA, 2016), apesar de ambos serem compostos considerados extremamente tóxicos e muito perigosos ao meio ambiente (CHEMINOVA, 2014; SYNGENTA, 2017). Alguns estudos foram realizados com embriões de Danio rerio expostos isoladamente a ivermectinas (composto do mesmo grupo químico da abamectina) e ao fungicida difenoconazol (DOMINGUES et al, 2016;MU et al, , 2015MU et al, , 2016OLIVEIRA et al, 2016), não sendo encontradas informações sobre os efeitos gerados pela exposição de embriões de Danio rerio à mistura binária de abamectina e difenoconazol. Desta forma, não se conhece o real efeito causado aos embriões quando estes são expostos às misturas destes agrotóxicos.…”

Section: Como Método Alternativo O Fet -Fish Embryo Toxicity Test Founclassified

“…3 -Dados das atividades das enzimas de biotransformação etoxiresorufina-O-deetilase (EROD), glucuronisil-transferase (UDPGT) e glutationa -Stransferase (GST); das enzimas de defesa antioxidante catalase (CAT), glutationa peroxidase (GPx) e glutationa redutase (GR); e peroxidação lipídica pela medida dos níveis malondialdeído (MDA) e hidroperóxidos lipídicos (HL) analisados em brânquias de Danio rerio expostos por 96h em mesocosmos ao runoff e spray drift dos agrotóxicos Kraft 36EC® + Score 250EC®. As médias e desvios estão expressos em nmol/min/mg de proteína (EROD, UDPGT, HL e MDA) e U/mg de proteína (GST, CAT, GPx e GR (DOMINGUES et al, 2016;MU et al, 2015MU et al, , 2016OLIVEIRA et al, 2016). Tabela 4.…”

Section: Análises Físico-químicas E Limnológicas Da áGua Dos Mesocosmosunclassified

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Avaliação do efeito ambiental do inseticida Kraft 36EC® (abamectina) e do fungicida Score 250EC® (difenoconazol) por meio de análises ecotoxicológicas em diferentes estágios de vida do <i>Danio rerio</i>

Sanches¹

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Exposure to thiourea during the early stages of development impedes the formation of the swim bladder in zebrafish larvae

Pillai,

Karandikar,

Pandya

et al. 2024

J of Applied Toxicology

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Thiourea, a widely used agrochemical, is known to inhibit the activity of thyroid peroxidase, a key enzyme in the biosynthetic pathway of thyroid hormones. Thyroid insufficiency compromises the basal metabolic rate in warm‐blooded organisms and embryonic development in vertebrates. In this study, we looked for developmental defects by exposing the zebrafish embryos to an environmentally relevant dose of thiourea (3 mg/mL). Liquid chromatography–tandem mass spectrometry (LC‐MS/MS) was performed to validate thiourea's presence in the treated zebrafish embryos. Structural anomalies like bent tail and pericardial edema were noticed in 96‐h post‐fertilization (hpf) larvae. On histological examination, underdeveloped swim bladder was noticed in 96 hpf larvae exposed to 3 mg/mL thiourea. The treated larvae also failed to follow the characteristic swimming behavior in response to stimuli due to defective swim bladder. Swim bladder being homologous to the lung of tetrapod, the role of Bmp4, a major regulator of lung development, was studied along with the associated regulatory genes. Gene expression analysis revealed that thiourea administration led to the downregulation of bmp4, shh, pcna, anxa5, acta2, and the downstream effector snail3 but the upregulation of caspase3. The protein expression showed a similar trend, wherein Bmp4, Shh, and Pcna were downregulated, but Cleaved Caspase3 showed an increased expression in the treated group. Therefore, it is prudent to presume that exposure to thiourea significantly reduces the expression of Bmp4 and other key regulators; hence, the larvae fail to develop a swim bladder, a vital organ that regulates buoyancy.

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Multilevel assessment of ivermectin effects using different zebrafish life stages

Cited by 46 publications

References 52 publications

Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos

Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos

Avaliação do efeito ambiental do inseticida Kraft 36EC® (abamectina) e do fungicida Score 250EC® (difenoconazol) por meio de análises ecotoxicológicas em diferentes estágios de vida do <i>Danio rerio</i>

Exposure to thiourea during the early stages of development impedes the formation of the swim bladder in zebrafish larvae

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