Multilayer fluorescence optically encoded beads for protein detection

Jun, Bong‐Hyun; Rho, Chul; Byun, Jang‐Woong; Kim, Jong-Ho; Chung, Woo‐Jae; Kang, Homan; Park, Ju‐Young; Cho, Suhyung; Kim, Byung-gee; Lee, Jun Young

doi:10.1016/j.ab.2009.05.052

Cited by 17 publications

(9 citation statements)

References 9 publications

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“…Doyle and co-workers have presented a method based on continuous-flow lithography that combines dot-patterned particle synthesis and probe conjugation into a single process to generate multifunctional encoded particles bearing over a million unique codes 19 . Optical encodings commonly rely on specific color or spectroscopic information of light emitted from several optical materials such as fluorescence dyes 7 23 28 29 , quantum dots (QDs) 12 20 , photonic structures 14 30 , and Raman tags 9 10 11 31 . Nie and co-workers have reported the optical encoding technology based on QDs entrapped into polystyrene microbeads by solvent swelling methods 12 .…”

mentioning

confidence: 99%

Direct Identification of On-Bead Peptides Using Surface-Enhanced Raman Spectroscopic Barcoding System for High-Throughput Bioanalysis

Kang

Jeong

Koh

et al. 2015

Sci Rep

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Recently, preparation and screening of compound libraries remain one of the most challenging tasks in drug discovery, biomarker detection, and biomolecular profiling processes. So far, several distinct encoding/decoding methods such as chemical encoding, graphical encoding, and optical encoding have been reported to identify those libraries. In this paper, a simple and efficient surface-enhanced Raman spectroscopic (SERS) barcoding method using highly sensitive SERS nanoparticles (SERS ID) is presented. The 44 kinds of SERS IDs were able to generate simple codes and could possibly generate more than one million kinds of codes by incorporating combinations of different SERS IDs. The barcoding method exhibited high stability and reliability under bioassay conditions. The SERS ID encoding based screening platform can identify the peptide ligand on the bead and also quantify its binding affinity for specific protein. We believe that our SERS barcoding technology is a promising method in the screening of one-bead-one-compound (OBOC) libraries for drug discovery.

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mentioning

confidence: 99%

Direct Identification of On-Bead Peptides Using Surface-Enhanced Raman Spectroscopic Barcoding System for High-Throughput Bioanalysis

Kang

Jeong

Koh

et al. 2015

Sci Rep

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“…As shown in Fig. 6, 10 forms of multi-layered fluorescent particles can be created using only two individual dyes (Jun et al 2010, 2012).…”

Section: Synthesis and Optical Encoding Of Microbeadsmentioning

confidence: 99%

Multiplexed microarrays based on optically encoded microbeads

Vafajoo

Rostami

Parsa

et al. 2018

Biomed Microdevices

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In recent years, there has been growing interest in optically-encoded or tagged functionalized microbeads as a solid support platform to capture proteins or nucleotides which may serve as biomarkers of various diseases. Multiplexing technologies (suspension array or planar array) based on optically encoded microspheres have made possible the observation of relatively minor changes in biomarkers related to specific diseases. The ability to identify these changes at an early stage may allow the diagnosis of serious diseases (e.g. cancer) at a time-point when curative treatment may still be possible. As the overall accuracy of current diagnostic methods for some diseases is often disappointing, multiplexed assays based on optically encoded microbeads could play an important role to detect biomarkers of diseases in a non-invasive and accurate manner. However, detection systems based on functionalized encoded microbeads are still an emerging technology, and more research needs to be done in the future. This review paper is a preliminary attempt to summarize the state-of-the-art concerning diagnostic microbeads; including microsphere composition, synthesis, encoding technology, detection systems, and applications.

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“…Especially, there was strong fluorescence in stratum corneum because isothiocyanate of FITC was interacted on major components of NMF (natural moisturizing factor) existed in keratinocyte [27]. To visualize the skin delivery of the liposome/hydrogel complex using Franz diffusion cell, we used confocal laser scanning microscope.…”

Section: In Vitro Skin Permeation Studymentioning

confidence: 99%

“…And, rhodamine B captured in the hydrophilic portion dispersed in the skin cell after fusion with cell membranes. Especially, there was strong fluorescence in stratum corneum because isothiocyanate of FITC was interacted on major components of NMF (natural moisturizing factor) existed in keratinocyte [27]. On the contrary, the stratum corneum was totally hydrated in comparison with liposomes when treated with hydrogels.…”

Section: In Vitro Skin Permeation Studymentioning

confidence: 99%