Multilayer Assembly of Prussian Blue Nanoclusters and Enzyme-Immobilized Poly(toluidine blue) Films and Its Application in Glucose Biosensor Construction

Dai, Zhicong; Zhang, Ke; Yao, Yan; Xia, Xueqi; Chen, Hong‐Yuan

doi:10.1021/la049667f

Cited by 119 publications

(61 citation statements)

References 36 publications

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“…[4][5][6] Therefore, different chemically modified electrodes have been developed for biomolecular analysis, environmental monitoring, and analysis of food quality. [7][8][9][10][11] Functional modification of electrode substrates for the construction of electrochemical biosensors having improved analytical performance with respect to other biosensor designs is highly desirable for developing potential device applications.…”

Section: Introductionmentioning

confidence: 99%

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Veerapandian¹,

Seo²,

Shin³

et al. 2012

IJN

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Abstract:A novel clinical glucose biosensor fabricated using functionalized metalloid-polymer (silver-silica coated with polyethylene glycol) hybrid nanoparticles on the surface of a graphene oxide nanosheet is reported. The cyclic voltammetric response of glucose oxidase modification on the surface of a functionalized graphene oxide electrode showed a surface-confined reaction and an effective redox potential near zero volts, with a wide linearity of 0.1-20 mM and a sensitivity of 7.66 µA mM. The functionalized graphene oxide electrode showed a better electrocatalytic response toward oxidation of H 2 O 2 and reduction of oxygen. The practical applicability of the functionalized graphene oxide electrode was demonstrated by measuring the peak current against multiple urine and serum samples from diabetic patients. This new hybrid nanoarchitecture combining a three-dimensional metalloid-polymer hybrid and two-dimensional graphene oxide provided a thin solid laminate on the electrode surface. The easy fabrication process and retention of bioactive immobilized enzymes on the functionalized graphene oxide electrode could potentially be extended to detection of other biomolecules, and have broad applications in electrochemical biosensing.

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Section: Introductionmentioning

confidence: 99%

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Veerapandian¹,

Seo²,

Shin³

et al. 2012

IJN

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“…At the reduction potential of H 2 O 2 , oxidation of interference species such as AA does not occur. [17][18][19] Here, we report an alternative approach for depleting interfering electroactive species based on the theory of the electrochemical diffusion layer. When the electrochemical Abstract: A dual-electrode configuration for the highly selective detection of glucose in the diffusion layer of the substrate electrode is presented.…”

Section: Introductionmentioning

confidence: 99%

A Dual‐Electrode Approach for Highly Selective Detection of Glucose Based on Diffusion Layer Theory: Experiments and Simulation

Wang

Dai

Zhou

et al. 2005

Chemistry A European J

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A dual-electrode configuration for the highly selective detection of glucose in the diffusion layer of the substrate electrode is presented. In this approach, a glassy carbon electrode (GCE, substrate) modified with a conductive layer of glucose oxidase/Nafion/graphite (GNG) was used to create an interference-free region in its diffusion layer by electrochemical depletion of interfering electroactive species. A Pt microelectrode (tip, 5 microm in radius) was located in the diffusion layer of the GNG-modified GCE (GNG-G) with the help of scanning electrochemical microscopy. Consequently, the tip of the electrode could sense glucose selectively by detecting the amount of hydrogen peroxide (H2O2) formed from the oxidization of glucose on the glucose oxidase layer. The influences of parameters, including tip-substrate distance, substrate potential, and electrolyzing time, on the interference-removing efficiency of this dual-electrode approach have been investigated systematically. When the electrolyzing time was 30 s, the tip-substrate distance was 1.8 a (9.0 microm) (where a is the radius of the tip electrode), the potentials of the tip and substrate electrodes were 0.7 V and 0.4 V, respectively, and a mixture of ascorbic acid (0.3 mM), uric acid (0.3 mM), and 4-acetaminophen (0.3 mM) had no influence on the glucose detection. In addition, the current-time responses of the tip electrode at different tip-substrate distances in a solution containing interfering species were numerically simulated. The results from the simulation are in good agreement with the experimental data. This research provides a concept of detection in the diffusion layer of a substrate electrode, as an interference-free region, for developing novel microelectrochemical devices.

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“…12,29 In subsidiary experiments we observed that bare ITO and ITO modified with PB used in amperometric measurements failed to detect lactose.…”

Section: ■ Results and Discussionmentioning

confidence: 92%

Amperometric Detection of Lactose Using β-Galactosidase Immobilized in Layer-by-Layer Films

Campos

Moraes

Volpati

et al. 2014

ACS Appl. Mater. Interfaces

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A direct, low-cost method to determine the concentration of lactose is an important goal with possible impact in various types of industry. In this study, a biosensor is reported that exploits the specific interaction between lactose and the enzyme β-galactosidase (β-Gal) normally employed to process lactose into glucose and galactose for lactose-intolerant people. The biosensor was made with β-Gal immobilized in layer-by-layer (LbL) films with the polyelectrolyte poly(ethylene imine) (PEI) and poly(vinyl sufonate) (PVS) on an indium tin oxide (ITO) electrode modified with a layer of Prussian Blue (PB). With an ITO/PB/(PEI/PVS) 1 (PEI/β-Gal) 30 architecture, lactose could be determined with an amperometric method with sensitivity of 0.31 μA mmol −1 cm −2 and detection limit of 1.13 mmol L −1, which is sufficient for detecting lactose in milk and for clinical exams. Detection occurred via a cascade reaction involving glucose oxidase titrated as electrolytic solution in the electrochemical cell, while PB allowed for operation at 0.0 V versus saturated calomel electrode, thus avoiding effects from interfering species. Sum-frequency generation spectroscopy data for the interface between the LbL film and a buffer containing lactose indicated that β-Gal lost order, which is the first demonstration of structural effects induced by the molecular recognition interaction with lactose.

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Multilayer Assembly of Prussian Blue Nanoclusters and Enzyme-Immobilized Poly(toluidine blue) Films and Its Application in Glucose Biosensor Construction

Cited by 119 publications

References 36 publications

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

A Dual‐Electrode Approach for Highly Selective Detection of Glucose Based on Diffusion Layer Theory: Experiments and Simulation

Amperometric Detection of Lactose Using β-Galactosidase Immobilized in Layer-by-Layer Films

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