2003
DOI: 10.1128/aac.47.10.3138-3144.2003
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Multilaboratory Comparison of Proficiencies in Susceptibility Testing of Helicobacter pylori and Correlation between Agar Dilution and E Test Methods

Abstract: Susceptibility testing was performed at seven Canadian microbiology laboratories and the Helicobacter Reference Laboratory, Halifax, Nova Scotia, Canada, to assess susceptibility testing proficiency and the reproducibility of the results for clarithromycin and metronidazole and to compare the Epsilometer test (E test) method to the agar dilution reference method. Control strain Helicobacter pylori ATCC 43504 (American Type Culture Collection) and 13 clinical isolates (plus duplicates of four of these strains i… Show more

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Cited by 48 publications
(41 citation statements)
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“…Both the Etest and PCR-RFLP detected the same four clarithromycin-resistant isolates, as has previously been reported (3,10). Results obtained by PCR-RFLP help to specify the mechanism of H. pylori clarithromycin resistance, and this technique could also be applied directly to stool or biopsy samples (18,22).…”
supporting
confidence: 84%
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“…Both the Etest and PCR-RFLP detected the same four clarithromycin-resistant isolates, as has previously been reported (3,10). Results obtained by PCR-RFLP help to specify the mechanism of H. pylori clarithromycin resistance, and this technique could also be applied directly to stool or biopsy samples (18,22).…”
supporting
confidence: 84%
“…The usual phenotypic methods for susceptibility testing can be applied to H. pylori (3,10). Numerous genotypic methods have been developed to detect clarithromycin resistance (16); the common methods used are PCR-restriction fragment length polymorphism (RFLP) and real-time PCR (17).…”
mentioning
confidence: 99%
“…These breakpoints were used based on the recommendations of the National Committee for Clinical Laboratory Standards (NCCLS) (11,(18)(19)(20)(21)(22).…”
Section: Resultsmentioning
confidence: 99%
“…The agar dilution method is the reference method proposed by the Clinical and Laboratory Standards Institute (CLSI), formerly the National Committee for Clinical Laboratory Standards, to be used for H. pylori antibiotic susceptibility testing (19,20). Alternatively, clinical laboratories use the Etest method as it is considered less technically demanding (21,22).…”
mentioning
confidence: 99%