Multigenotype Q Fever Outbreak, the Netherlands

Klaassen, Corné H. W.; Nabuurs-Franssen, Marrigje H.; Tilburg, Jeroen J. H. C.; Hamans, Maurice A.W.M.; Horrevorts, A. M.

doi:10.3201/eid1504.081612

Cited by 67 publications

(62 citation statements)

References 3 publications

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“…Strong positive samples (with Ct values below 30) were further genotyped with multispacer sequence typing (MST) based on 10 loci and multilocus variablenumber tandem repeat analysis (MLVA) based on six loci [13][14][15].…”

Section: Methodsmentioning

confidence: 99%

Q fever epidemic in Hungary, April to July 2013

et al. 2014

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We investigated a Q fever outbreak with human patients showing high fever, respiratory tract symptoms, headache and retrosternal pain in southern Hungary in the spring and summer of 2013. Seventy human cases were confirmed by analysing their serum and blood samples with micro-immunofluorescence test and real-time PCR. The source of infection was a merino sheep flock of 450 ewes, in which 44.6% (25/56) seropositivity was detected by enzyme-linked immunosorbent assay. Coxiella burnetii DNA was detected by real-time PCR in the milk of four of 20 individuals and in two thirds (41/65) of the manure samples. The multispacer sequence typing examination of C. burnetii DNA revealed sequence type 18 in one human sample and two manure samples from the sheep flock. The multilocus variable-number tandem repeat analysis pattern of the sheep and human strains were also almost identical,

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Section: Methodsmentioning

confidence: 99%

Q fever epidemic in Hungary, April to July 2013

et al. 2014

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“…Clinical samples from other patients from different locations in the same high-risk area were 5 9 13 17 21 22 29 33 37 41 45 49 1 5 9 13 17 21 25 29 33 37 41 45 49 1 5 9 13 17 21 25 29 33 37 41 45 49 53 4 8 12 included in the study. All genotypes showed a high degree of similarity with most genotypes differing from each other by only a single marker, suggesting a clonal origin (J. J. H. C. Tilburg & C. H. W. Klaassen, unpublished observations) [33,34]. Eventually, 1000 human Q fever cases were notified in 2008 (Fig.…”

Section: Continuation Of the Human Outbreakmentioning

confidence: 99%

“…In the years 2007 and 2008, it became clear that Q fever also posed a problem to public health. The connection between Q fever problems in the dairy goat and dairy sheep industry and in the human population was made by several authors based on epidemiological and C. burnetii typing-based findings [28,33,35]. The increase in goat density took place in the highly populated province of NoordBrabant (average population density in NoordBrabant is 497 inhabitants/km 2 , compared to an average of 398 inhabitants/km 2 for The Netherlands).…”

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confidence: 99%

The Q fever epidemic in The Netherlands: history, onset, response and reflection

et al. 2010

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SUMMARYThe 2007-2009 human Q fever epidemic in The Netherlands attracted attention due to its magnitude and duration. The current epidemic and the historical background of Q fever in The Netherlands are reviewed according to national and international publications. Seroprevalence studies suggest that Q fever was endemic in The Netherlands several decades before the disease was diagnosed in dairy goats and dairy sheep.

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“…Certain PCR amplification primers or detection probes do not match the target sequences of certain genomes equally well (Table 5). Depending on the location of the present mismatch(es), this may have had a detrimental effect on the performance of the (6). Therefore, DNA sequence polymorphisms in the IS1111a elements in this Dutch clone may also affect the different PCR assays studied here and may also explain the poor performance of the PCR used by laboratory G. Whether or not this is a likely explanation remains to be established.…”

Section: Discussionmentioning

confidence: 85%

Interlaboratory Evaluation of Different Extraction and Real-Time PCR Methods for Detection ofCoxiella burnetiiDNA in Serum

et al. 2010

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In the Netherlands, there is an ongoing and unparalleled outbreak of Q fever. Rapid and reliable methods to identify patients infected with Coxiella burnetii, the causative agent of Q fever, are urgently needed. We evaluated the performance of different DNA extraction methods and real-time PCR assays that are in use in seven diagnostic or reference laboratories in the Netherlands. A low degree of variation in the sensitivities of most of the developed real-time PCR assays was observed. However, PCR assays amplifying short DNA fragments yielded better results than those producing large DNA fragments. With regard to DNA extraction, the automated MagNA Pure Compact system and the manual QIAamp DNA mini kit consistently yielded better results than either the MagNA Pure LC system and NucliSens EasyMag (both automated) or the High Pure viral nucleic acid kit (manual). The present study shows that multiple combinations of DNA extraction kits and real-time PCR assays offer equivalent solutions to detect C. burnetii DNA in serum samples from patients suspected to have Q fever.

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Multigenotype Q Fever Outbreak, the Netherlands

Cited by 67 publications

References 3 publications

Q fever epidemic in Hungary, April to July 2013

Q fever epidemic in Hungary, April to July 2013

The Q fever epidemic in The Netherlands: history, onset, response and reflection

Interlaboratory Evaluation of Different Extraction and Real-Time PCR Methods for Detection ofCoxiella burnetiiDNA in Serum

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