Multigenic control of Listeria monocytogenes susceptibility in mice

Abstract

We have used a novel quantitative trait locus model to study the genetics of survival of F2 progeny of susceptible BALB/cByJ and resistant C57BL/6ByJ mice that have been infected with Listeria monocytogenes. This allowed us to map modifiers of L. monocytogenes susceptibility to chromosomes 5 and 13.

Genetic regulation of host responses to Salmonella infection in mice

“…Now the future of mouse genetics in the study of host resistance to Salmonella and other infections is moving toward the identification of inbred mouse strains that show a complex mode of inheritance to specific pathogens for QTL gene identification. 19,20,[153][154][155][156][157][158][159][160] Dissection of the complex host response to Salmonella infection combined with the complete mouse genome sequence will contribute further to our understanding of the genetic control of host immunity.…”

Tyrosine 759 of the cytokine receptor gp130 is involved in Listeria monocytogenes susceptibility

“…Supporting this idea, microsatellite typing (Table 1) revealed that both B6.gp130 WT/WT and B6.gp130 F759/F759 mice, which exhibited the significant difference in Listeria resistance (Figure 7), possessed the D13Mit99-D13Mit147 region as C57BL/6-type alleles, known to have the advantageous effect on Listeria infection in C57BL/6ByJ mice. 21 In addition, the carriage of the 129/Sv-derived region in B6.gp130 F759/F759 mice was not extended as far as the D13Mit104 marker (Table 1). This result reduces the possibility that the enhanced susceptibility in gp130 F759/F759 mice is due to the genetic variations between 129/Sv and C57BL/6 strains although the effect of non-segregated 129/Sv-derived region cannot be completely excluded.…”

“…21 The murine gp130 locus has been tentatively mapped to chromosome 13 (D13Ertd699e/3950 marker, the UniSTS database, http://www.ncbi.nlm.nih.gov/genome/sts/), suggesting that gp130 associates with the Listeria susceptibility loci. To identify the murine gp130 locus experimentally, we carried out fluorescence in situ hybridization (FISH) analysis.…”

“…It therefore should be considered whether or not a different chimerism of 129/Sv and C57BL/6 genetic backgrounds influenced the different susceptibility observed between gp130 WT/WT and gp130 F759/F759 mice. To examine this possibility, gp130 F759/F759 mice were backcrossed to C57BL/6 for Another recent report by Boyartchuk et al 21 revealed that the region between D13Mit99 and D13Mit147 on chromosome 13 in C57BL/6ByJ (resistant) strain appears to have the dominant and advantageous effect on the survival of mice infected with Listeria. To examine the possibility that the enhanced susceptibility in B6.gp130 F759/F759 mice is due to a lack of the C57BL/6ByJ-type resistant allele(s) between D13Mit99 and D13Mit147, the carriage of 129-derived regions after seven-times backcross were estimated by typing of microsatellite markers in chromosome 13.…”

“…The murine gp130 locus was estimated to be 18 to 27 cM apart from the Listeria susceptibility region on chromosome 13 (D13Mit99-D13Mit147), 21 implying that gp130 would be another locus affecting Listeria resistance. Supporting this idea, microsatellite typing (Table 1) revealed that both B6.gp130 WT/WT and B6.gp130 F759/F759 mice, which exhibited the significant difference in Listeria resistance (Figure 7), possessed the D13Mit99-D13Mit147 region as C57BL/6-type alleles, known to have the advantageous effect on Listeria infection in C57BL/6ByJ mice.…”