Multigenerational exposure of the microalga Tetraselmis suecica to diuron leads to spontaneous long-term strain adaptation

Stachowski-Haberkorn, Sabine; Jérôme, Marc; Rouxel, Julien; Khelifi, Célia; Rincé, Maéva; Burgeot, Thierry

doi:10.1016/j.aquatox.2013.06.016

Cited by 40 publications

(21 citation statements)

References 57 publications

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“…Another consequence of photosynthesis inhibition is the potential accumulation of intracellular ROS, as previously described in many studies (Knauert and Knauer, 2008;Prado et al, 2012;Stachowski-Haberkorn et al, 2013;Esperanza et al, 2015;Dupraz et al, 2016). In the present study, significant effects, measured using either H2DCFDA or dihydroethidium, were observed after exposure to all individual substances ( Figures 3, 4 and 5A).…”

Section: Effects Of the Single Substances On Physiological Parameterssupporting

confidence: 85%

“…Some chemicals are known to modify the fluorescence of microalgal pigments (Prado et al, 2011;Stachowski-Haberkorn et al, 2013), as also shown in the present study by the measurement of FL1 and FL2 fluorescence…”

Section: Toxicity Of Single Substances In Microplates and Culture Flaskssupporting

confidence: 80%

“…In addition to cell density measurements, flow cytometry can be used to analyse several morphological parameters, such as relative cell size and complexity and relative fluorescence, which is related to pigment content (Marie et al, 2005;Stachowski-Haberkorn et al, 2013;Mansano et al, 2017). Using fluorescent dyes specific to particular physiological functions, several studies have shown the usefulness of flow cytometry to indicate the toxicity of certain chemicals to microalgae (Prado et al, 2009(Prado et al, , 2012Rioboo et al, 2011;Stachowski-Haberkorn et al, 2013;Seoane et al, 2014Seoane et al, , 2017Esperanza et al, 2015;Dupraz et al, 2016;González-Pleiter et al, 2017). The most commonly investigated physiological functions in these studies are viability, general metabolic activity (often measured by non-specific esterase activity) and reactive oxygen species (ROS), whose accumulation following exposure to certain contaminants can cause oxidative stress and thus cell damage.…”

Section: A C C E P T E Dmentioning

confidence: 99%

“…Today their use is only permitted as films or construction material preservatives (European Chemicals Agency, 2018). Their high toxicity towards microalgae has been reported in numerous studies (Arzul et al, 2006;Bao et al, 2011;Stachowski-Haberkorn et al, 2013;Sjollema et al, 2014;Dupraz et al, 2016Dupraz et al, , 2018. Metazachlor is a herbicide belonging to the chloroacetamide family that inhibits the synthesis of very long chain fatty acids (VLCFAs) thus disturbing cell division (Götz and Böger, 2004;Vallotton et al, 2008).…”

Section: A C C E P T E Dmentioning

confidence: 99%

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Toxicity of binary mixtures of pesticides to the marine microalgae Tisochrysis lutea and Skeletonema marinoi: Substance interactions and physiological impacts

et al. 2019

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This study screened binary mixtures of pesticides for potential synergistic interaction effects on growth of the marine microalgae Tisochrysis lutea and Skeletonema marinoi. It also examined the single and combined effects of three of the most toxic substances on microalgal physiology.Single substances were first tested on each microalgal species to determine their respective EC 50 and concentration-response relationships. The toxicity of six and seven binary mixtures was then evaluated in microplate experiments on the growth of T. lutea and S. marinoi, respectively, using two mixture modelling approaches: isobolograms and the MIXTOX tool, based on Concentration Addition (CA) or Independent Action (IA) models. Significant cases of antagonism (for both species) and synergism (for S. marinoi) were observed for the mixtures of isoproturon and spiroxamine, and isoproturon and metazachlor, respectively.These two mixtures, together with that of isoproturon and diuron, for which additivity was observed, were further studied for their impacts on the physiology of each species. Exposures were thus made in culture flasks at three concentrations, or concentration combinations for mixtures, selected to cause 25%, 50% and 75% growth rate inhibition. The effects of the selected pesticides singly and in combination were evaluated at three perceived effect concentrations on esterase metabolic activity, relative lipid content, cytoplasmic membrane potential and reactive oxygen species (ROS) content by flow cytometry, and on photosynthetic quantum yield (ϕ' M ) by PAM-fluorescence.Isoproturon and diuron singly and in mixtures induced 20-40% decreases in ϕ' M which was in turn responsible for a significant decrease in relative lipid content for both species. Spiroxamine and metazachlor were individually responsible for an increase in relative lipid content (up to nearly 300% for Highlights ► The toxicity of binary pesticide mixtures was evaluated on two microalgal species. ► Effects of three selected mixtures were then examined on five physiological functions. ► Effects on microalgal physiology were linked to the pesticide's toxic mode of action. ► Mixed isoproturon and metazachlor had significant synergistic effects on S. marinoi. ► Observed synergy could be due to a combined effect on the photosynthetic apparatus.

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Section: Effects Of the Single Substances On Physiological Parameterssupporting

confidence: 85%

Section: Toxicity Of Single Substances In Microplates and Culture Flaskssupporting

confidence: 80%

Section: A C C E P T E Dmentioning

confidence: 99%

Section: A C C E P T E Dmentioning

confidence: 99%

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Toxicity of binary mixtures of pesticides to the marine microalgae Tisochrysis lutea and Skeletonema marinoi: Substance interactions and physiological impacts

et al. 2019

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“…A T. suecica strain resistant to diuron (diuron-resistant mutant: T_mutant) was obtained from wild strain CCMP 904 after experiments performed by Stachowski-Haberkorn et al (2013).…”

Section: Microalgal Culturesmentioning

confidence: 99%

Microalgal sensitivity varies between a diuron-resistant strain and two wild strains when exposed to diuron and irgarol, alone and in mixtures

Dupraz¹,

Coquillé²,

Ménard³

et al. 2016

Chemosphere

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A wild strain of Chaetoceros calcitrans and wild and diuron-resistant strains of Tetraselmis suecica, were exposed to the PSII inhibitor herbicides diuron and irgarol, individually and in mixtures. The effects of three concentrations of diuron and irgarol and four binary mixtures were evaluated on doubling time, relative reactive oxygen species and lipid content by flow cytometry, and on photosynthetic efficiency by pulse amplitude modulated fluorescence. In both wild strains, significant effects were observed for each molecule at the highest concentration tested: at irgarol 0.5 μg L(-1), C. calcitrans was shown to be more sensitive than T. suecica (+52% and +19% in doubling time, respectively), whereas at diuron 5 μg L(-1), T. suecica was more affected (+125% in doubling time) than C. calcitrans (+21%). Overall, irgarol had a higher toxicity at a lower concentration than diuron (no effect at diuron 0.5 μg L(-1)) for both wild strains. The strongest mixture (irgarol 0.5 μg L(-1) + diuron 5 μg L(-1)) increased doubling time by 356% for T. suecica, thus showing amplified effects when the two compounds were mixed. Sequencing of the diuron-resistant strain demonstrated a single mutation in the psbA gene coding sequence. Although resistance of this strain to diuron was confirmed with no effect at the highest diuron concentration, no resistance to irgarol was shown. In addition, the mutant strain exposed to the strongest mixture showed a 3.5-fold increase in doubling time compared with irgarol alone, thereby supporting the hypothesis of a biochemical interaction between these two compounds.

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Monoculture and co-culture tests of the toxicity of four typical herbicides on growth, photosynthesis and oxidative stress responses of the marine diatoms Pseudo-nitzschia mannii and Chaetoceros decipiens

et al. 2022

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The toxicity of four herbicides in mixture (alachlor, diuron, des-isopropyl-atrazine and 31 simazine) on the growth and the photosynthesis parameters of two marine diatoms Pseudo-32 niszchia mannii and Chaetoceros decipiens have been investigated for 9 days in monoculture 33 and co-culture tests. The catalase (CAT) and guaiacol peroxidase (GPX) were also monitored 34 to assess the oxidative stress response. In single-species assays, while both species displayed 35 no affected instantaneous growth rate by herbicides, their physiological responses were 36 different. Chl a content of P. mannii significantly decreased upon herbicide exposure, due 37 probably to pigment destruction or inhibition of their synthesis. This decrease was associated 38 with a reduction in the chlorophyll fluorescence parameters (ABS0/RC, TR0/RC, ET0/RC and 39 DI0/RC). In contrast, C. decipiens maintained an effective photosynthetic performance under 40 herbicide exposure, as Chl a per cell content and the specific energy fluxes per reaction center 41 remained unchanged relative to control values. GPX activity was significantly higher in 42 contaminated P. mannii and C. decipiens monocultures than in controls at early herbicide 43 exposure (1 day), whereas a significant induction of CAT activity occurred later (from day 3 44 for C. decipiens and at day 9 for P. mannii) in response to herbicides. In control co-culture, P.45 mannii was eliminated by C. decipiens. As observed in the monoculture, the herbicides did not 46 affect the photosynthetic performance of C. decipiens in co-culture, but significantly reduced 47 its instantaneous growth rate. The oxidative stress response in co-culture has similar trends to 48 that of C. decipiens in monoculture, but the interspecies competition likely resulted in higher 49 CAT activity under herbicide exposure. Results of this study suggest that herbicide toxicity for 50 marine diatoms might be amplified by interspecies interactions in natural communities, which 51 might lead to different physiological and growth responses.

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Multigenerational exposure of the microalga Tetraselmis suecica to diuron leads to spontaneous long-term strain adaptation

Cited by 40 publications

References 57 publications

Toxicity of binary mixtures of pesticides to the marine microalgae Tisochrysis lutea and Skeletonema marinoi: Substance interactions and physiological impacts

Toxicity of binary mixtures of pesticides to the marine microalgae Tisochrysis lutea and Skeletonema marinoi: Substance interactions and physiological impacts

Microalgal sensitivity varies between a diuron-resistant strain and two wild strains when exposed to diuron and irgarol, alone and in mixtures

Monoculture and co-culture tests of the toxicity of four typical herbicides on growth, photosynthesis and oxidative stress responses of the marine diatoms Pseudo-nitzschia mannii and Chaetoceros decipiens

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