Multigenerational effects of carbendazim in Daphnia magna: From a subcellular to a population level

Abstract

Anthropogenic activities such as the use of pesticides may affect aquatic biota populations, due to potential agricultural runoffs or disposals. Carbendazim is one example of a widely used fungicide with a high potential to end up in aquatic ecosystems through runoff. Deleterious effects observed at the individual level are possibly explained by changes in homeostasis at the cellular level, and both factors can then be used to predict effects at the population level. In the present study, an isoclonal population of Daphnia magna (clone K6) was exposed to a concentration that mimics relevant levels of carbendazim in the environment over 12 generations. The effects of carbendazim were assessed in some generations using the following endpoints: biochemical biomarkers (cholinesterase, catalase, and glutathione-S-transferase), lipid peroxidation and energy-related parameters (carbohydrates, lipids, and proteins along with available energy and energy consumption), parental longevity, and population growth (r). Long-term exposure to carbendazim had no effect on the intrinsic rate of natural increase (r) of adult D. magna, but longevity was decreased at the F12 generation compared to that of control. Differences between the exposed and nonexposed populations were found for cholinesterase, glutathione-S-transferase, and lipid peroxidation. However, for catalase and energy-related parameters, no differences were observed between these 2 populations. Natural variability was seen throughout the test period, under control conditions, within the 12 generations. Overall, carbendazim induced some effects at the subcellular level that translated into changes in longevity but these later vanished in terms of population effects. Environ Toxicol Chem 2019;38:412-422. C 2018 SETAC

A maternal effect influences sensitivity to chlorpyrifos pesticide in the pest moth Spodoptera littoralis

“…A small portion of ecotoxicological literature is dedicated to the impact of pesticides on several generations (Brausch and Salice, 2011;Sarkar et al, 2018;Silva et al, 2019), but studies on the transgenerational effects of other factors on sensitivity of pesticides are particularly limited. A study on the mosquito C. pipiens revealed an effect of the temperature experienced by the parental generation (interacting with parental pesticide exposure) on offspring sensitivity to chlorpyrifos pesticide (Tran et al, 2018).…”

“…The impact of transgenerational effects on sensitivity to pesticides is poorly studied. Most related studies are on the effects of pesticides on several generations (Brausch and Salice, 2011;Sarkar et al, 2018;Silva et al, 2019). However, studies of other transgenerational effects on pesticide sensitivity are especially scarce (Pieters and Liess, 2006;Tran et al, 2018Tran et al, , 2019.…”

Acute toxicity characterization of organic UV-filters and chronic exposure revealing multigenerational effects in DAPHNIA MAGNA

“…The reproductive effects observed for the F1 generation exposed to environmental concentrations of UV lters should be considered of ecological relevance, since a reproductive delay and a decreased reproductive rate may potentially impact at the populational level. Thus, different levels of the biological organization can be affected and cause chain effects in future populations, as continuous exposure to contaminants directly affects the health of new individuals (Campos et al 2016;Silva et al 2019).…”

Daphnia magna Multigeneration Exposure to Carbendazim: Gene Transcription Responses

“…Considering the above, the present study aimed to assess the effects of long-term exposure to carbendazim (5 µg L −1 ) on D. magna at the gene transcription level using a multigenerational approach with twelve generations (F0-F12). The selection of the carbendazim concentration considered the concentrations found in surface waters, with a particular focus on the upper limit of the range [6] and previous toxicity tests [22][23][24]. Multigenerational effects of carbendazim in D. magna-specific subcellular (DNA damage and biochemical biomarkers) and individual/populational level endpoints (such as longevity/reproduction) were previously evaluated by us [24].…”