Multigene phylogeny of Malagasy day geckos of the genus Phelsuma

Rocha, Sara; Vences, Miguel; Glaw, Frank; Posada, David; Harris, D. James

doi:10.1016/j.ympev.2009.03.032

Cited by 46 publications

(44 citation statements)

References 21 publications

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“…All but one individual were genotyped for a fragment of the mitochondrial gene cytochrome-b (cyt-b), and a subset of these (see Appendix S1) for five nuclear fragments (C-mos, Rag2, MC1R, PDC and RELNint61). For cyt-b, a fragment of c. 800 bp was initially amplified with primers CBL14753 (Austin, Arnold & Jones, 2004) and CB3H (Palumbi et al, 1991), and conditions as in Rocha et al (2009b). Specific primers for this species were later designed (UroF, 5′-CAAAAACCTAATGACCCCACTACG-3′; UroR, 5′-TAAAGTTTTCTGGGTCTCCTAG-3′; forward and reverse, respectively) and used to amplify a fragment of 730 bp.…”

Section: Sampling and Molecular Methodsmentioning

confidence: 99%

Cryptic diversity within the endemic prehensile-tailed geckoUrocotyledon inexpectataacross the Seychelles Islands: patterns of phylogeographical structure and isolation at the multilocus level

Rocha

Harris²,

Posada

2011

Biological Journal of the Linnean Society

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The deciphering of the process of genetic differentiation of species with insular distributions is relevant for biogeographical and conservation reasons. Despite their importance as old gondwanic islands and part of the western Indian Ocean biodiversity hotspot, little is known about the genetic structure of taxa from the Seychelles Islands. We have examined the patterns of structure and isolation within Urocotyledon inexpectata (Reptilia: Geckkonidae), an endemic species from this archipelago. Genetic diversity was screened from populations across the archipelago for both mitochondrial and nuclear genes. Gene genealogies and model-based inference were used to explore patterns and timings of isolation between the main lineages. High levels of genetic diversity were found for the mitochondrial and some of the nuclear markers. This species harbours at least two highly differentiated lineages, exclusively distributed across the northern and southern groups of the islands. The main split between these was dated back to the Miocene-late Pliocene, but isolation events throughout the Pliocene and Pleistocene were also inferred. Migration between groups of islands was apparently nonexistent, except for one case. The low dispersal capabilities of this species, together with the intrinsic fragmented nature of its geographical distribution, seem to have resulted in highly structured populations, despite the cyclic periods of contact between the different island groups. These populations may currently represent more than one species, making U. inexpectata another example of a morphologically cryptic lineage with deep genetic divergence within gekkonids. The observed patterns suggest a hypothetical biogeographic scenario (of a main north-south phylogeographic break) for the Seychelles that can be further tested with the exploration of the phylogeographic structure of other Seychellois taxa.

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Section: Sampling and Molecular Methodsmentioning

confidence: 99%

Cryptic diversity within the endemic prehensile-tailed geckoUrocotyledon inexpectataacross the Seychelles Islands: patterns of phylogeographical structure and isolation at the multilocus level

Rocha

Harris²,

Posada

2011

Biological Journal of the Linnean Society

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“…Amplifications were conducted in 15-25 lL reactions using standard conditions with annealing temperatures of 50-52°C and a magnesium concentration of 1-1.5 mM. For PDC we used primers and conditions as in Gamble et al (2008), for C-mos we used primers Mos-F and Mos-R and conditions as in Godinho et al (2005) and RAG2 fragment amplification was as in Rocha et al (2009b). Primers and conditions for MC1R and RELNint61 are given in Pinho et al (2010).…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

Evolutionary history ofTrachylepisskinks in the Seychelles islands: introgressive hybridization, morphological evolution and geographic structure

Rocha

Perera²,

Silva³

et al. 2016

Biol. J. Linn. Soc.

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The Seychelles is a remarkably interesting archipelago for evolutionary studies, but only recently have molecular markers been used to explore its biogeographic patterns. Here we used morphological and molecular data to examine diversity and phylogenetic relationships of two endemic skink sister-species from this archipelago: Trachylepis sechellensis and Trachylepis wrightii. Mitochondrial DNA genealogy rendered a monophyletic T. wrightii nested within a paraphyletic T. sechellensis, whereas nuclear DNA sequences from five unlinked markers reflected the accepted taxonomy. Hybridization and massive mtDNA introgression leading to the complete replacement of the native mtDNA lineage of T. sechellensis in some of the islands were invoked to explain this result, and morphological variation also seemed to reflect this pattern of reticulation. A Mio-Pliocene divergence between both species is suggested. Multilocus molecular data were used to uncover biogeographic patterns within the archipelago, which reflected shared patterns with other co-distributed lizard taxa; specifically a north-south marked structure, a close relationship between populations from Fregate and the southern islands, and a detectable isolation within the southern group, between Mah e, and Silhouette and North Islands. Gene flow from these latter islands towards the northern group was also suggested. These results add to the growing body of evidence of the influence of geographic distance and sea-level oscillations in shaping the genetic structure of Seychellois taxa and of the existence of common biogeographic patterns across the archipelago.

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“…Coetivy island is a small sand cay (9.3 km²), apparently lacking endemics (Stuart and RJ 1990). Agalega consists of two islands with a total land area of 24 km² and is home to an endemic subspecies of the Réunion day gecko, (Phelsuma borbonica agalegae) (Cheke 2010;Rocha et al 2009), Cargados Carajos is a group of tiny islets with a total land area of 1.3 km² and no terrestrial endemism. Tromelin is a tiny rock (0.8 km²), but is a host to some apparently endemic insects, such as the homopteran Pulvinaria tromelini (Stoddart 1970), and is the stronghold breeding place of a western Indian Ocean endemic subspecies of terns (Sula dactylatra melanops).…”

Section: The 'Minor' Islands Of the Western Indian Oceanmentioning

confidence: 99%

The Generation of a Biodiversity Hotspot: Biogeography and Phylogeography of the Western Indian Ocean Islands

Agnarsson¹,

Kuntner²

2012

Current Topics in Phylogenetics and Phylogeography of Terrestrial and Aquatic Systems

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Multigene phylogeny of Malagasy day geckos of the genus Phelsuma

Cited by 46 publications

References 21 publications

Cryptic diversity within the endemic prehensile-tailed geckoUrocotyledon inexpectataacross the Seychelles Islands: patterns of phylogeographical structure and isolation at the multilocus level

Cryptic diversity within the endemic prehensile-tailed geckoUrocotyledon inexpectataacross the Seychelles Islands: patterns of phylogeographical structure and isolation at the multilocus level

Evolutionary history ofTrachylepisskinks in the Seychelles islands: introgressive hybridization, morphological evolution and geographic structure

The Generation of a Biodiversity Hotspot: Biogeography and Phylogeography of the Western Indian Ocean Islands

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