Multigene phylogenies of Ophiostomataceae associated with Monterey pine bark beetles in Spain reveal three new fungal species

Romón, Pedro; Beer, Z. Wilhelm de; Zhou, Xudong; Duong, Tuan A.; Wingfield, Brenda D.; Wingfield, Michael J.

doi:10.3852/13-073

Cited by 19 publications

(22 citation statements)

References 52 publications

(43 reference statements)

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“…The other four species from pine bark beetles come from single reports: S. rossi from the USA (Davidson 1971), S. aurorae from South Africa (Zhou et al. 2006), and two species from Spain (Romón et al., 2014a, Romón et al., 2014b). Interestingly, the S. gossypina complex is the only species complex in Sporothrix, apart from Lineage G, which includes species associated with conifer-infesting bark beetles.…”

Section: Discussionmentioning

confidence: 99%

The divorce of Sporothrix and Ophiostoma: solution to a problematic relationship

Beer

Duong

Wingfield

2016

Studies in Mycology

165

134

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One of the causal agents of human sporotrichosis, Sporothrix schenckii, is the type species of the genus Sporothrix. During the course of the last century the asexual morphs of many Ophiostoma spp. have also been treated in Sporothrix. More recently several DNA-based studies have suggested that species of Sporothrix and Ophiostoma converge in what has become known as Ophiostoma s. lat. Were the one fungus one name principles adopted in the Melbourne Code to be applied to Ophiostoma s. lat., Sporothrix would have priority over Ophiostoma, resulting in more than 100 new combinations. The consequence would be name changes for several economically important tree pathogens including O. novo-ulmi. Alternatively, Ophiostoma could be conserved against Sporothrix, but this would necessitate changing the names of the important human pathogens in the group. In this study, we sought to resolve the phylogenetic relationship between Ophiostoma and Sporothrix. DNA sequences were determined for the ribosomal large subunit and internal transcribed spacer regions, as well as the beta-tubulin and calmodulin genes in 65 isolates. The results revealed Sporothrix as a well-supported monophyletic lineage including 51 taxa, distinct from Ophiostoma s. str. To facilitate future studies exploring species level resolution within Sporothrix, we defined six species complexes in the genus. These include the Pathogenic Clade containing the four human pathogens, together with the S. pallida-, S. candida-, S. inflata-, S. gossypina- and S. stenoceras complexes, which include environmental species mostly from soil, hardwoods and Protea infructescences. The description of Sporothrix is emended to include sexual morphs, and 26 new combinations. Two new names are also provided for species previously treated as Ophiostoma.

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Section: Discussionmentioning

confidence: 99%

The divorce of Sporothrix and Ophiostoma: solution to a problematic relationship

Beer

Duong

Wingfield

2016

Studies in Mycology

165

134

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“…The common practice of culturing specimens from insects and placement into phylogenetic trees obtained for the ITS region (e.g., Romon et al. ) already constitutes a useful framework for assessing the taxonomic diversity. The ITS marker exploits highly conserved primer recognition sequences for amplification, which reduces taxonomic bias, while the intervening central region is sufficiently variable for species‐level diagnostics.…”

Section: Discussionmentioning

confidence: 99%

Metabarcoding of fungal communities associated with bark beetles

Miller

Hopkins

Inward

et al. 2016

Ecology and Evolution

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Many species of fungi are closely allied with bark beetles, including many tree pathogens, but their species richness and patterns of distribution remain largely unknown. We established a protocol for metabarcoding of fungal communities directly from total genomic DNA extracted from individual beetles, showing that the ITS3/4 primer pair selectively amplifies the fungal ITS. Using three specimens of bark beetle from different species, we assess the fungal diversity associated with these specimens and the repeatability of these estimates in PCRs conducted with different primer tags. The combined replicates produced 727 fungal Operational Taxonomic Units (OTUs) for the specimen of Hylastes ater, 435 OTUs for Tomicus piniperda, and 294 OTUs for Trypodendron lineatum, while individual PCR reactions produced on average only 229, 54, and 31 OTUs for the three specimens, respectively. Yet, communities from PCR replicates were very similar in pairwise comparisons, in particular when considering species abundance, but differed greatly among the three beetle specimens. Different primer tags or the inclusion of amplicons in separate libraries did not impact the species composition. The ITS2 sequences were identified with the Lowest Common Ancestor approach and correspond to diverse lineages of fungi, including Ophiostomaceae and Leotiomycetes widely found to be tree pathogens. We conclude that Illumina MiSeq metabarcoding reliably captures fungal diversity associated with bark beetles, although numerous PCR replicates are recommended for an exhaustive sample. Direct PCR from beetle DNA extractions provides a rapid method for future surveys of fungal species diversity and their associations with bark beetles and environmental variables.

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“…Duong et al (2012) suggested that this species belongs to the Grosmannia serpens complex, but validation of the name will be possible only if the original material can be located . Other fungi in this group, Ophiostoma sejunctum (Villarreal et al 2005), Ophiostoma nebulare, Ophiostoma euskadiense and Graphilbum crescericum (Romón et al 2014) were described only very recently, suggesting that these fungi have been incompletely studied in the area.…”

Section: Introductionmentioning

confidence: 95%

“…Knowledge of the ophiostomatoid fungi in the Iberian Peninsula is very limited (De Ana Magán 1982;1983;Fernández et al 2004;Villarreal et al 2005;Romón et al 2007;Bueno et al 2010;Pestaña and Santolamazza-Carbone 2010;Romón et al 2014), and only three studies have dealt with their taxonomy. For example, a new species, Leptographium gallaeiciae, was described invalidly from stressed Pinus pinaster trees (De Ana Magán 1983).…”

Section: Introductionmentioning

confidence: 98%

Ophiostomatoid fungi including two new fungal species associated with pine root-feeding beetles in northern Spain

Romón

Beer

Fernández

et al. 2014

Antonie van Leeuwenhoek

Self Cite

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Many bark beetles live in a symbiosis with ophiostomatoid fungi but very little is known regarding these fungi in Spain. In this study, we considered the fungi associated with nine bark beetle species and one weevil infesting two native tree species (Pinus sylvestris and Pinus nigra) and one non-native (Pinus radiata) in Cantabria (Northern Spain). This included examination of 239 bark beetles or their galleries. Isolations yielded a total of 110 cultures that included 11 fungal species (five species of Leptographium sensu lato including Leptographium absconditum sp. nov., five species of Ophiostoma sensu lato including Ophiostoma cantabriense sp. nov, and one species of Graphilbum). The most commonly encountered fungal associates of the bark beetles were Grosmannia olivacea, Leptographium procerum, and Ophiostoma canum. The aggressiveness of the collected fungal species was evaluated using inoculations on two-year-old P. radiata seedlings. Leptographium wingfieldii, Leptographium guttulatum, and Ophiostoma ips were the only species capable of causing significant lesions.

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Multigene phylogenies of Ophiostomataceae associated with Monterey pine bark beetles in Spain reveal three new fungal species

Cited by 19 publications

References 52 publications

The divorce of Sporothrix and Ophiostoma: solution to a problematic relationship

The divorce of Sporothrix and Ophiostoma: solution to a problematic relationship

Metabarcoding of fungal communities associated with bark beetles

Ophiostomatoid fungi including two new fungal species associated with pine root-feeding beetles in northern Spain

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