Multigene CRISPR/Cas9 genome editing of hybrid proline rich proteins (HyPRPs) for sustainable multi-stress tolerance in crops: the review of a promising approach

Saikia, Banashree; Singh, Sanjay; Debbarma, Johni; Velmurugan, Natarajan; Dekaboruah, Hariprasanna; Arunkumar, Kallare P.; Chikkaputtaiah, Channakeshavaiah

doi:10.1007/s12298-020-00782-6

Cited by 16 publications

(11 citation statements)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proline-rich proteins (PRPs) was found to be upregulated in the tolerant pool of populations, and some evidence suggests that PRPs is responsible for cell wall structure, such as GhHyPRP4, which may be involved in the plant response to cold stress in cotton [30]; Brassica BnPRP genes could be induced by cold [31]; and the Arabidopsis HyPRP gene protects the cells during freezing stress [32]. In this study, the higher expression of PRP genes, leading to proline accumulation, may be because the increase in proline added mechanical strength to the cell wall and stabilized the structure of organs under low temperature stress [33,34].…”

Section: Discussionmentioning

confidence: 71%

BSR-Seq Analysis Provides Insights into Cold Stress in Actinidia Arguta F1 Populations

Lin¹,

Sun²,

Fang³

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundThe freezing injury, which is one of the important abiotic stresses in horticultural crops, can influences the growth and development and the production area of kiwifruit (Actinidia Lind1). Actinidia arguta has excellent cold resistance in Actinidia species, but knowledge relevant to molecular mechanisms is still limited so far. Understanding the mechanism of cold resistance in kiwifruit is important for cold-resistant breeding. ResultsIn our study, a cross of ‘Ruby-3’×‘Kuilv’ male was built for the A. arguta hardiness study, and 20 cold-tolerant and 20 cold-sensitive populations were selected from 492 populations according to LT50. Then, we performed Bulked segregant RNA-seq combined with single-molecule real-time sequencing to identify differentially expressed genes with cold hardiness. We found that the content of soluble sucrose and the activity of β-amylase were higher in the cold tolerant population pool than in the cold sensitive population pool. Upon -30°C low temperature treatment, 126 differentially expressed genes were found, and 59 genes were upregulated and 67 genes were downregulated when comparing the tolerant and sensitive pools, respectively. KEGG pathway analysis showed that the DEGs mainly belonged to starch and sucrose metabolism and amino sugar and nucleotide sugar metabolism. There were main 10 key enzyme encoding genes and two regulatory genes were up regulated in tolerant pool, regulated genes of CBF pathway were found to be different expressed, especially, 14-3-3 gene was down regulated and EBF gene was up regulated. To validate the BSR-Seq results, 24 DEGs were determined by qRT-PCR, and the results were consistent with BSR-Seq.ConclusionOur research provides valuable insights into the mechanism related to cold resistance in Actinidia and identified potential genes that are important for cold resistance in kiwifruit.

show abstract

Section: Discussionmentioning

confidence: 71%

BSR-Seq Analysis Provides Insights into Cold Stress in Actinidia Arguta F1 Populations

Lin¹,

Sun²,

Fang³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Proline-rich proteins ( PRPs ) were found to be up-regulated in the tolerant pool of populations, and some evidence suggests that PRPs are responsible for cell wall structure, such as GhHyPRP4 , which may be involved in the plant response to cold stress in cotton [ 31 ]; Brassica BnPRP genes could be induced by cold [ 32 ]; and the Arabidopsis HyPRP gene protects the cells during freezing stress [ 33 ]. In this study, the higher expression of PRP genes, leading to proline accumulation, may be because the increase in proline added mechanical strength to the cell wall and stabilized the structure of organs under low temperature stress [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

BSR-Seq analysis provides insights into the cold stress response of Actinidia arguta F1 populations

et al. 2021

View full text Add to dashboard Cite

Background Freezing injury, which is an important abiotic stress in horticultural crops, influences the growth and development and the production area of kiwifruit (Actinidia Lind1). Among Actinidia species, Actinidia arguta has excellent cold resistance, but knowledge relevant to molecular mechanisms is still limited. Understanding the mechanism underlying cold resistance in kiwifruit is important for breeding cold resistance. Results In our study, a population resulting from the cross of A. arguta ‘Ruby-3’ × ‘Kuilv’ male was generated for kiwifruit hardiness study, and 20 cold-tolerant and 20 cold-sensitive populations were selected from 492 populations according to their LT50. Then, we performed bulked segregant RNA-seq combined with single-molecule real-time sequencing to identify differentially expressed genes that provide cold hardiness. We found that the content of soluble sucrose and the activity of β-amylase were higher in the cold-tolerant population than in the cold-sensitive population. Upon − 30 °C low-temperature treatment, 126 differentially expressed genes were identify; the expression of 59 genes was up-regulated and that of 67 genes was down-regulated between the tolerant and sensitive pools, respectively. KEGG pathway analysis showed that the DEGs were primarily related to starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism. Ten major key enzyme-encoding genes and two regulatory genes were up-regulated in the tolerant pool, and regulatory genes of the CBF pathway were found to be differentially expressed. In particular, a 14–3-3 gene was down-regulated and an EBF gene was up-regulated. To validate the BSR-Seq results, 24 DEGs were assessed via qRT-PCR, and the results were consistent with those obtained by BSR-Seq. Conclusion Our research provides valuable insights into the mechanism related to cold resistance in Actinidia and identified potential genes that are important for cold resistance in kiwifruit.

show abstract

“…Taken together, our results indicate that SlHyPRP1 and DEA1 are predominantly localized to plasma membrane and cytoplasm. In vivo protein-protein interaction of SlHyPRP1 and DEA1 with stress associated proteins Multiple stress responses in plants are highly complex which might be regulated by several host genes (Saikia et al 2020). There could be a cross talk of plant-abiotic stress factors, plant-microbial interaction and plant defense pathway genes (Jones et al 2019).…”

Section: Analysis Of Sub-cellular Localization Of Slhyprp1 and Dea1mentioning

confidence: 99%

“…HyPRPs present in seed plants belongs to the family of 8CM containing PRPs (Li et al 2016). Apart from plant developmental processes, HyPRPs play important roles in response to different abiotic and biotic stresses (Yang et al 2018;Kapoor et al 2019;Saikia et al 2020). The N-terminal domain of HyPRPs resembles the structural cell wall PRPs but lacks the characteristic amino acid motifs of PRPs (José-Estanyol et al 2004;Dvořáková Lenka 2007).…”

Section: Introductionmentioning

confidence: 99%

“…The N-terminal domain of HyPRPs resembles the structural cell wall PRPs but lacks the characteristic amino acid motifs of PRPs (José-Estanyol et al 2004;Dvořáková Lenka 2007). Several studies in plants have shown that HyPRPs such as CaHyPRP1, GbHyPRP1, NbHyPRP1, GhHyPRP4, CcHyPRP1, AZI1, OsPRP, and BnPRP play multiple functional roles during specific developmental stage and respond to biotic and abiotic stresses as positive and negative regulators (Goodwin et al 1996;Gothandam and Flower 2010;Gengqing et al 2011;Jung et al 2012;Yeom et al 2012;Li et al 2016;Mellacheruvu et al 2016;Saikia et al 2020). Recently, the SlHyPRP1 (S. lycopersicum) and SpHyPRP1 (S. pennellii) genes of tomato were found to be negative regulators of different abiotic stresses such as drought, salinity, cold, oxidative stress, and phytohormone ABA (Li et al 2016;Yang et al 2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

SlHyPRP1 and DEA1, the multiple stress responsive eight-cysteine motif family genes of tomato (Solanum lycopersicum L.) are expressed tissue specifically, localize and interact at cytoplasm and plasma membrane in vivo

Saikia

Debbarma

Maharana

et al. 2020

Physiol Mol Biol Plants

Self Cite

View full text Add to dashboard Cite

Owing to rapid global climate change, the occurrence of multiple abiotic stresses is known to influence the outburst of biotic stress factors which affects crop productivity. Therefore, it is essential to understand the molecular and cell biology of key genes associated with multiple stress responses in crop plants. SlHyPRP1 and DEA1, the members of eight-cysteine motif (8CM) family genes have been recently identified as putative regulators of multiple stress responses in tomato (Solanum lycopersicum L.). In order to gain deeper insight into cell and molecular biology of SlHyPRP1 and DEA1, we performed their expression analysis in three tomato cultivars and in vivo cell biological analysis. The semi-quantitative PCR and qRT-PCR results showed the higher expression of SlHyPRP1 and DEA1 in leaf, stem, flower and root tissues as compared to fruit and seed tissues in all three cultivars. The expression levels of SlHyPRP1 and DEA1 were found to be relatively higher in a wilt susceptible tomato cultivar (Arka Vikas) than a multiple disease resistant cultivar (Arka Abhed). In vivo cell biological analysis through Gateway cloning and Bi-FC assay revealed the Electronic supplementary material The online version of this article (

show abstract

Multigene CRISPR/Cas9 genome editing of hybrid proline rich proteins (HyPRPs) for sustainable multi-stress tolerance in crops: the review of a promising approach

Cited by 16 publications

References 110 publications

BSR-Seq Analysis Provides Insights into Cold Stress in Actinidia Arguta F1 Populations

BSR-Seq Analysis Provides Insights into Cold Stress in Actinidia Arguta F1 Populations

BSR-Seq analysis provides insights into the cold stress response of Actinidia arguta F1 populations

SlHyPRP1 and DEA1, the multiple stress responsive eight-cysteine motif family genes of tomato (Solanum lycopersicum L.) are expressed tissue specifically, localize and interact at cytoplasm and plasma membrane in vivo

Contact Info

Product

Resources

About